TFIIE alpha (GTF2E1) (NM_005513) Human Untagged Clone

SKU SC116700

GTF2E1 (untagged)-Human general transcription factor IIE, polypeptide 1, alpha 56kDa (GTF2E1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TFIIE alpha
• Synonyms: FE; TF2E1; TFIIE-A
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116700 sequence for NM_005513 edited (data generated by NextGen Sequencing); ATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAAGTAT; GTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAGGAAC; TCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACTTCGA; TCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGAGACT; GCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCTTGTT; AATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAGAGAT; TCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTTAGAA; GCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATACAGAG; GTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAGGTTT; AATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTTGGCC; TATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGACCAT; GCAGCAACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGAAGCATGGGCC; ACCAAAGGTCCTTCCTATGAAGACTTATACACTCAGAATGTTGTCATTAACATGGATGAC; CAAGAAGATCTTCATCGAGCCTCACTGGAAGGGAAATCTGCCAAAGAGAGGCCTATTTGG; TTGAGAGAAAGCACTGTCCAAGGGGCATATGGTTCTGAAGATATGAAAGAAGGGGGCATA; GATATGGACGCATTTCAGGAGCGTGAGGAAGGCCATGCTGGGCCTGATGACAACGAAGAG; GTCATGCGAGCACTGCTCATTCACGAGAAAAAGACTTCCTCTGCCATGGCTGGTTCAGTG; GGGGCAGCTGCTCCAGTGACCGCTGCCAATGGCAGTGACTCAGAAAGCGAGACCAGTGAG; TCAGATGATGATTCTCCACCCCGTCCGGCAGCTGTGGCTGTGCATAAACGAGAAGAGGAT; GAAGAGGAAGATGACGAGTTTGAAGAAGTAGCAGATGACCCCATTGTCATGGTGGCTGGC; CGTCCGTTCTCCTACAGTGAAGTGAGCCAACGGCCAGAGCTAGTGGCCCAGATGACACCA; GAAGAAAAGGAAGCATATATAGCAATGGGACAACGCATGTTTGAGGACCTCTTTGAGTGA; ; Clone variation with respect to NM_005513.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005513 unedited; GGGTCACATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGCGCTGGGGG; CAGCTGTAGTGGGAGTGTTCCAGGATTCGCCTTGTATATTTAAAGTTGGAGTTCGTTGCT; AAAGATGGCAGACCCAGATGTCCTCACTGAAGTTCCAGCAGCATTGAAGCGGTTAGCCAA; GTATGTGATCCGGGGATTTTATGGCATTGAGCATGCCTTGGCCTTGGACATCTTGATCAG; GAACTCCTGTGTGAAAGAGGAGGATATGCTGGAGCTGCTCAAGTTTGATCGGAAGCAACT; TCGATCAGTTTTGAATAATTTAAAGGGAGACAAGTTTATCAAATGCAGAATGAGGGTAGA; GACTGCTGCAGACGGGAAAACCACTCGCCATAACTACTACTTCATCAATTATCGTACTCT; TGTTAATGTGGTAAAATATAAACTGGACCACATGAGAAGAAGAATTGAGACCGATGAGAG; AGATTCGACCAACCGGGCTTCCTTCAAATGTCCTGTCTGTAGTAGTACTTTCACAGACTT; AGAAGCTAATCAGCTCTTTGATCCTATGACAGGAACTTTCCGCTGTACTTTTTGCCATAC; AGAGGTAGAAGAGGATGAATCAGCAATGCCCAAAAAAGATGCACGCACACTTTTGGCAAG; GTTTAATGAACAAATTGAGCCCATTTATGCATTGCTTCGGGAGACAGAGGATGTGAACTT; GGCCTATGAAATACTTGAGCCAGAACCCACAGAAATCCCAGCCCTGAAACAGAGCAAGGA; CCATGCAGNCACTACTGCTGGAGCTGCTAGCCTAGCAGGTGGGCACCACCGGGGAGCATG; GGCCACCAAAGGTCCTTTCTATGAAGACTTATACACTCAGAATGTTGTCATTTACATGGA; TGACCAAGAAGATCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005513 unedited; GTATTTGTCACATTCAGTCTTAACACAACCCGGTAAATGAAACATCACTCCATACAAGGC; TCATATATTCTTTACATGTGTACTATAAAATGATAAATACTTAAAAATTACAATGCAAGG; AAAAATATTGTAAGATATACAATTGTTACAGACCCTTGTGTGAAAGTTCTTTATTTTTAA; AGTTCTGCCTTAGGGAAAATACCATGCTACATATCAGAACCCAAAATAAACTGTTTCCTG; AGGTTACAATGCTCAATGGTATACTGGATGCACCCTAAAATACTGTGTCTGACAAAAATT; AAAAGAGAGACAGCCTTCAATGATGTTTAGAAAGAGGGAGAGTGACAGAGAGAGAGAAGA; GTAATCAATTATTCTGTTGATTTCTGCTGGAATGGCCTCATTAAACAATTTATAGCACTG; ACAAGAGGTCTGTACCCAAAGGGAACAAAACCAGGACCAGTCTCAGCTTTACATAAAGGA; TTTCCAAATCAGACTAACTGACACAGTTCTAAGGCACTGTTACCAATGCTTGTCACTCTT; CAACCCTTCACAGAGACTTCTTGTCTTACGTCCATGTTTTTCTAATTTCTTCCTAAGGGT; TTACCAATTATACTTCTGTTTTCTATCATCCTAAATCCCAACCAAAAGACATTAANGCAT; ACAATAAAATCTACCAGTCCTGGTCAGTTCTAAAGACACAAAATCTGGTTTAGACATTAT; TCATAGACTCAGTTTATNTTTCCCCTTTCAACACGCTCCCATTNCCCTTCATATTGATCC; ATCTAATTTTGAGGAATGCTCAATTCTAAGCAAAATTCAGGAATTTTTCCCCATAAGGAT; TAGACTGTGGAAACTGTTTTGGTGGGCCCCATAAAAATTGNTGGAAGCCTGCTTGAAAAA; TAAAGAGGGGACAGGATTTTGGACCTCTGGTTAACCAAGGTGGAAGGTTACCTTGAACTC; CCTCCTTCTTTAAGGGAAAATTTTTTTTTAAGAGAACAAATAAAA;
• Restriction Sites: NotI-NotI
• ACCN: NM_005513
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005513.1, NP_005504.1
• RefSeq Size: 2969 bp
• RefSeq ORF: 1320 bp
• Locus ID: 2960
• UniProt ID: P29083
• Cytogenetics: 3q13.33
• Domains: TFIIE
• Protein Families: Transcription Factors
• Protein Pathways: Basal transcription factors
• Summary: Recruits TFIIH to the initiation complex and stimulates the RNA polymerase II C-terminal domain kinase and DNA-dependent ATPase activities of TFIIH. Both TFIIH and TFIIE are required for promoter clearance by RNA polymerase.[UniProtKB/Swiss-Prot Function]