Bestrophin 3 (BEST3) (NM_152439) Human Tagged ORF Clone

SKU RC218436

BEST3 (Myc-DDK-tagged)-Human bestrophin 3 (BEST3), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Bestrophin 3
• Synonyms: VMD2L3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218436 representing NM_152439; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGACAACAGATGAAAGGAAATTATTCAACCACCTCAAGTCTCCTCATCTGAAATATTGGGTTCCATTCA; TCTGGTTTGGAAATCTTGCAACTAAAGCCCGGAATGAAGGTAGAATCAGAGACAGTGTTGATCTGCAATC; ATTGATGACTGAAATGAATCGATACCGCTCTTGGTGCAGCCTCTTATTCGGTTATGACTGGGTTGGGATT; CCGCTGGTTTACACCCAGGTAGCAGAGCAGCTTATCAACCCTTTTGGAGAAGATGATGATGATTTTGAAA; CTAACTGGTGCATTGACAGAAATTTGCAGGTCTCTCTTTTAGCTGTGGACGAAATGCACATGAGCTTACC; CAAGATGAAGAAGGACATTTACTGGGACGATTCTGCTGCTCGCCCACCATACACATTGGCAGCTGCTGAC; TACTGCATACCCTCATTTCTGGGGTCAACAGTCCAGATGGGGCTGTCTGGGTCCGACTTTCCTGACGAGG; AGTGGCTGTGGGATTATGAGAAGCATGGCCATCGGCATTCCATGATAAGAAGAGTCAAGCGGTTCCTGAG; TGCCCACGAACACCCCTCCAGCCCCAGAAGAAGAAGCTACAGGAGGCAGACAAGTGACAGCTCCATGTTC; TTACCCCGAGATGACCTCAGCCCAGCCAGGGACCTACTGGATGTGCCCTCAAGAAACCCCCCCAGGGCCT; CACCCACCTGGAAGAAATCCTGCTTCCCAGAAGGAAGCCCCACGCTGCACTTCAGCATGGGAGAGCTGTC; CACCATCAGGGAGACCAGCCAGACAAGCACTTTACAGAGCCTGACCCCACAGTCCAGTGTGAGAACTTCC; CCCATCAAAATGCCACTGGTACCTGAGGTATTGATCACAGCAGCCGAAGCACCAGTGCCCACATCAGGGG; GCTACCACCATGATTCCGCTACCTCCATCTTGAGCTCTGAGTTTACAGGGGTTCAGCCAAGCAAGACTGA; GCAGCAGCAGGGCCCCATGGGATCCATCCTGTCTCCCTCAGAGAAGGAGACACCTCCTGGAGGCCCCAGT; CCCCAGACAGTTTCAGCCAGCGCTGAGGAAAATATATTCAACTGTGAAGAAGACCCTGGTGATACCTTTC; TAAAAAGGTGGAGTCTTCCGGGATTCCTGGGGTCCAGCCACACTTCCCTGGGAAACCTAAGTCCAGACCC; CATGAGCTCTCAGCCAGCTCTTTTAATTGACACAGAAACATCCTCAGAGATCAGTGGGATCAACATTGTG; GCTGGCTCTCGAGTCTCTTCTGATATGCTGTATTTAATGGAAAACCTGGACACCAAGGAAACAGATATCA; TAGAGCTGAACAAGGAAACTGAGGAATCACCCAAA; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218436 representing NM_152439; Red=Cloning site Green=Tags(s); ; MTTDERKLFNHLKSPHLKYWVPFIWFGNLATKARNEGRIRDSVDLQSLMTEMNRYRSWCSLLFGYDWVGI; PLVYTQVAEQLINPFGEDDDDFETNWCIDRNLQVSLLAVDEMHMSLPKMKKDIYWDDSAARPPYTLAAAD; YCIPSFLGSTVQMGLSGSDFPDEEWLWDYEKHGHRHSMIRRVKRFLSAHEHPSSPRRRSYRRQTSDSSMF; LPRDDLSPARDLLDVPSRNPPRASPTWKKSCFPEGSPTLHFSMGELSTIRETSQTSTLQSLTPQSSVRTS; PIKMPLVPEVLITAAEAPVPTSGGYHHDSATSILSSEFTGVQPSKTEQQQGPMGSILSPSEKETPPGGPS; PQTVSASAEENIFNCEEDPGDTFLKRWSLPGFLGSSHTSLGNLSPDPMSSQPALLIDTETSSEISGINIV; AGSRVSSDMLYLMENLDTKETDIIELNKETEESPK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152439
• ORF Size: 1365 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_152439.3, NP_689652.2
• RefSeq Size: 2898 bp
• RefSeq ORF: 1368 bp
• Locus ID: 144453
• UniProt ID: Q8N1M1
• Cytogenetics: 12q15
• Protein Families: Ion Channels: Other, Transmembrane
• MW: 50.8 kDa
• Summary: BEST3 belongs to the bestrophin family of anion channels, which includes BEST1 (MIM 607854), the gene mutant in vitelliform macular dystrophy (VMD; MIM 153700), and 2 other BEST1-like genes, BEST2 (MIM 607335) and BEST4 (MIM 607336). Bestrophins are transmembrane (TM) proteins that share a homology region containing a high content of aromatic residues, including an invariant arg-phe-pro (RFP) motif. The bestrophin genes share a conserved gene structure, with almost identical sizes of the 8 RFP-TM domain-encoding exons and highly conserved exon-intron boundaries. Each of the 4 bestrophin genes has a unique 3-prime end of variable length (Stohr et al., 2002 [PubMed 12032738]; Tsunenari et al., 2003 [PubMed 12907679]).[supplied by OMIM, Mar 2008]