SEC14 like protein 2 (SEC14L2) (NM_012429) Human Tagged ORF Clone

SKU
RC215994
SEC14L2 (Myc-DDK-tagged)-Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1
  • TrueORF Gold

    Protein expression verified by Western blot, fully sequenced and in stock

    Click here to learn more.

$686.00
In Stock*
Specifications
Product Data
Target Symbol SEC14 like protein 2
Synonyms C22orf6; SPF; TAP; TAP1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215994 representing NM_012429
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAGCGGCAGAGTCGGCGATCTGAGCCCCAGGCAGAAGGAGGCATTGGCCAAGTTTCGGGAGAATGTCC
AGGATGTGCTGCCGGCCCTGCCGAATCCAGATGACTATTTTCTCCTGCGTTGGCTCCGAGCCAGAAGCTT
CGACCTGCAGAAGTCGGAGGCCATGCTCCGGAAGCATGTGGAGTTCCGAAAGCAAAAGGACATTGACAAC
ATCATTAGCTGGCAGCCTCCAGAGGTGATCCAACAGTATCTGTCAGGGGGTATGTGTGGCTATGACCTGG
ATGGCTGCCCAGTCTGGTACGACATAATTGGACCTCTGGATGCCAAGGGTCTGCTGTTCTCAGCCTCCAA
ACAGGACCTGCTGAGGACCAAGATGCGGGAGTGTGAGCTGCTTCTGCAAGAGTGTGCCCACCAGACCACA
AAGTTGGGGAGGAAGGTGGAGACCATCACCATAATTTATGACTGCGAGGGGCTTGGCCTCAAGCATCTCT
GGAAGCCTGCTGTGGAGGCCTATGGAGAGTTTCTCTGCATGTTTGAGGAAAATTATCCCGAAACACTGAA
GCGTCTTTTTGTTGTTAAAGCCCCCAAACTGTTTCCTGTGGCCTATAACCTCATCAAACCCTTCCTGAGT
GAGGACACTCGTAAGAAGATCATGGTCCTGGGAGCAAATTGGAAGGAGGTTTTACTGAAACATATCAGCC
CTGACCAGGTGCCTGTGGAGTATGGGGGCACCATGACTGACCCTGATGGAAACCCCAAGTGCAAATCCAA
GATCAACTACGGGGGTGACATCCCCAGGAAGTATTATGTGCGAGACCAGGTGAAACAGCAGTATGAACAC
AGCGTGCAGATTTCCCGTGGCTCCTCCCACCAAGTGGAGTATGAGATCCTCTTCCCTGGCTGTGTCCTCA
GGTGGCAGTTTATGTCAGATGGAGCGGATGTTGGTTTTGGGATTTTCCTGAAGACCAAGATGGGAGAGAG
GCAGCGGGCAGGGGAGATGACAGAGGTGCTGCCCAACCAGAGGTACAACTCCCACCTGGTCCCTGAAGAT
GGGACCCTCACCTGCAGTGATCCTGGCATCTATGTCCTGCGGTTTGACAACACCTACAGCTTCATTCATG
CCAAGAAGGTCAATTTCACTGTGGAGGTCCTGCTTCCAGACAAAGCCTCAGAAGAGAAGATGAAACAGCT
GGGGGCAGGCACCCCGAAA


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215994 representing NM_012429
Red=Cloning site Green=Tags(s)

MSGRVGDLSPRQKEALAKFRENVQDVLPALPNPDDYFLLRWLRARSFDLQKSEAMLRKHVEFRKQKDIDN
IISWQPPEVIQQYLSGGMCGYDLDGCPVWYDIIGPLDAKGLLFSASKQDLLRTKMRECELLLQECAHQTT
KLGRKVETITIIYDCEGLGLKHLWKPAVEAYGEFLCMFEENYPETLKRLFVVKAPKLFPVAYNLIKPFLS
EDTRKKIMVLGANWKEVLLKHISPDQVPVEYGGTMTDPDGNPKCKSKINYGGDIPRKYYVRDQVKQQYEH
SVQISRGSSHQVEYEILFPGCVLRWQFMSDGADVGFGIFLKTKMGERQRAGEMTEVLPNQRYNSHLVPED
GTLTCSDPGIYVLRFDNTYSFIHAKKVNFTVEVLLPDKASEEKMKQLGAGTPK

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_012429
ORF Size 1209 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_012429.5
RefSeq Size 2818 bp
RefSeq ORF 1212 bp
Locus ID 23541
UniProt ID O76054
Cytogenetics 22q12.2
Domains SEC14
Protein Families Transcription Factors
MW 46 kDa
Summary This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]
Write Your Own Review
You're reviewing:SEC14 like protein 2 (SEC14L2) (NM_012429) Human Tagged ORF Clone
Your Rating
SKU Description Size Price
RC215994L1 Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC215994L2 Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, mGFP tagged 10 ug
$986.00
RC215994L3 Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, Myc-DDK-tagged 10 ug
$986.00
RC215994L4 Lenti ORF clone of Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1, mGFP tagged 10 ug
$986.00
RG215994 SEC14L2 (tGFP-tagged) - Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1 10 ug
$886.00
SC115384 SEC14L2 (untagged)-Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1 10 ug
$457.00

Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.