SEC14 like protein 2 (SEC14L2) (NM_012429) Human Untagged Clone

SKU SC115384

SEC14L2 (untagged)-Human SEC14-like 2 (S. cerevisiae) (SEC14L2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SEC14 like protein 2
• Synonyms: C22orf6; SPF; TAP; TAP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC115384 sequence for NM_012429 edited (data generated by NextGen Sequencing); ATGAGCGGCAGAGTCGGCGATCTGAGCCCCAAGCAGAAGGAGGCATTGGCCAAGTTTCGG; GAGAATGTCCAGGATGTGCTGCCGGCCCTGCCGAATCCAGATGACTATTTTCTCCTGCGT; TGGCTCCGAGCCAGAAGCTTCGACCTGCAGAAGTCGGAGGCCATGCTCCGGAAGCATGTG; GAGTTCCGAAAGCAAAAGGACATTGACAACATCATTAGCTGGCAGCCTCCAGAGGTGATC; CAACAGTATCTGTCAGGGGGTATGTGTGGCTATGACCTGGATGGCTGCCCAGTCTGGTAC; GACATAATTGGACCTCTGGATGCCAAGGGTCTGCTGTTCTCAGCCTCCAAACAGGACCTG; CTGAGGACCAAGATGCGGGAGTGTGAGCTGCTTCTGCAAGAGTGTGCCCACCAGACCACA; AAGTTGGGGAGGAAGGTGGAGACCATCACCATAATTTATGACTGCGAGGGGCTTGGCCTC; AAGCATCTCTGGAAGCCTGCTGTGGAGGCCTATGGAGAGTTTCTCTGCATGTTTGAGGAA; AATTATCCCGAAACACTGAAGCGTCTTTTTGTTGTTAAAGCCCCCAAACTGTTTCCTGTG; GCCTATAACCTCATCAAACCCTTCCTGAGTGAGGACACTCGTAAGAAGATCATGGTCCTG; GGAGCAAATTGGAAGGAGGTTTTACTGAAACATATCAGCCCTGACCAGGTGCCTGTGGAG; TATGGGGGCACCATGACTGACCCTGATGGAAACCCCAAGTGCAAATCCAAGATCAACTAC; GGGGGTGACATCCCCAGGAAGTATTATGTGCGAGACCAGGTGAAACAGCAGTATGAACAC; AGCGTGCAGATTTCCCGTGGCTCCTCCCACCAAGTGGAGTATGAGATCCTCTTCCCTGGC; TGTGTCCTCAGGTGGCAGTTTATGTCAGATGGAGCGGATGTTGGTTTTGGGATTTTCCTG; AAGACCAAGATGGGAGAGAGGCAGCGGGCAGGGGAGATGACAGAGGTGCTGCCCAACCAG; AGGTACAACTCCCACCTGGTCCCTGAAGATGGGACCCTCACCTGCAGTGATCCTGGCATC; TATGTCCTGCGGTTTGACAACACCTACAGCTTCATTCATGCCAAGAAGGTCAATTTCACT; GTGGAGGTCCTGCTTCCAGACAAAGCCTCAGAAGAGAAGATGAAACAGCTGGGGGCAGGC; ACCCCGAAATAA; ; Clone variation with respect to NM_012429.3; 32 g=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012429 unedited; NAAGAGGTCTANATTTGTATACGACTCCTATAGGGCGGCCGCGAATTCGCACGAGGGCGG; CGAGGACGAGTCTGTGCTCCATCAGCTGCCGCACCCGCCGCCTCCCGCCCCCAAACCCCA; TCCCCGCGGTTGAGCCACGATGAGCGGCAGAGTCGGCGATCTGAGCCCCAAGCAGAAGGA; GGCATTGGCCAAGTTTCGGGAGAATGTCCAGGATGTGCTGCCGGCCCTGCCGAATCCAGA; TGACTATTTTCTCCTGCGTTGGCTCCGAGCCAGAAGCTTCGACCTGCAGAAGTCGGAGGC; CATGCTCCGGAAGCATGTGGAGTTCCGAAAGCAAAAGGACATTGACAACATCATTAGCTG; GCAGCCTCCAGAGGTGATCCCTCAGTATCTGTCAGGGGGTATGTGTGGCTATGACCTGGA; TGGCTGCCCAGTCTGGTACGACATAATTGGACCTCTGGATGCCAAGGGTCTGCTGTTCTC; AGCCTCCAAACAGGACCTGCTGAGGACCAAGATGCGGGAGTGTGAGCTGCTTCTGCAAGA; GTGTGCCCACCAGACCCAAAGTTGGGGAGGAAGGTGGAGACCATCACCATAATTTATGAC; TGCCAGGGGCTTGGCCTCAAGCATCTCTGGAAGCCTGCTGTGGAGGCCTATGGAGAGTTT; CTCTGCATGTTTGAGGAAAATTATCCCGAAACACTGAAGCGTCTTTTTGTTGTTAAAGCC; CCCAAACTGTTTCCTGTGGNCCTATACCTCATCAAACCCTTCCTGAGTGAGGACACTCGT; AAGAAGACATGGTCCTGGGGAGCAATGGAAGGAGTTTTACTGAACATATCACCCTGACCA; GTGCCTGTGAGTTGGGGGCACATGACTGACCTGATGGAACCCN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012429 unedited; GCGGCACGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGCGCACTGAACGTTGCTTT; ATTCATTGGTTAATTTTCCTAACAGCGTTGTAAACCCAGGCCGGGATGTCCTGAGCGTTC; TGGCAGAGGCCCGTGCAGCCTCGGCCCCTTCCGGTCCGCGCTAGCCTGGCCTTTGCCCTG; ACTCCCTCAGTTTGCAAAGAGAGCTTCCCAAAAAGGGGCCGGGGCCCTGCCTCTGGAAGG; AGAAGGCTTTCCCCCCCAGTCCTGGGGGCCCCCTTCCTTGTTCCTTAAACCCCCCCCCCC; GGGGTCTCTTGGCGGGGGCCCTGGGGACTGGGGTAGGGGCTCGGTTCTCAGGGCCCTTTG; CCCCCCCCCCACCCCCTTTTCGCGAAAACCCCCCCCCCCCCCTGCGCGGACTCCCCCGCG; GGGGACCCATACCCGCCCACACCACCGCACCCTCCCCCCCCCCCAACACAAACTCCCCCT; CTCCCTCAGTGGCGCCCTCTCCCCACCCCCTCCCCCTTCCCCTCCCCCTCCCCCCCACCT; TTCCTATCTCTCTCCCCCCCCCCCCCCCCCCCCCCCCCCCCTCCTCCTCTTCCCCCCTCC; TCTCTTCCCCCCCCCTCTCCTCCCCTCAGCTTCTCCTCCCCCCCACCTCCTCCCCTCCCC; TTCTCACTCCCCCTTCCCCCTCCCCCTCCCTTTCCCCCCCCCTCATCTCCCACCCCCATC; CCATTTTCACGACCTTTGTCCGCATCGCCATCTTCCCGCTTCCCTTCGCCCCCCACCCCC; CCCCCCCCCCCCATCCTCGTTTTCCATTTTTTGTTCCCTCCCCCACCCT;
• Restriction Sites: NotI-NotI
• ACCN: NM_012429
• Insert Size: 2820 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_012429.1, NP_036561.1
• RefSeq Size: 2818 bp
• RefSeq ORF: 1212 bp
• Locus ID: 23541
• UniProt ID: O76054
• Cytogenetics: 22q12.2
• Domains: SEC14
• Protein Families: Transcription Factors
• Summary: This gene encodes a cytosolic protein which belongs to a family of lipid-binding proteins including Sec14p, alpha-tocopherol transfer protein, and cellular retinol-binding protein. The encoded protein stimulates squalene monooxygenase which is a downstream enzyme in the cholesterol biosynthetic pathway. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Oct 2008]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.