Collagen XV (COL15A1) (NM_001855) Human Tagged ORF Clone

SKU RC215981

COL15A1 (Myc-DDK-tagged)-Human collagen, type XV, alpha 1 (COL15A1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Collagen XV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215981 representing NM_001855; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCACCAAGGAGGAACAACGGGCAGTGCTGGTGTCTGCTGATGCTGCTCTCGGTCTCCACGCCCCTCC; CTGCTGTCACCCAGACCCGCGGTGCGACAGAGACTGCTTCCCAGGGTCACCTGGACCTCACGCAGCTCAT; CGGTGTCCCGCTGCCCTCGTCCGTATCCTTTGTCACAGGCTATGGTGGCTTCCCGGCCTACAGTTTCGGG; CCTGGTGCCAATGTTGGCCGCCCAGCCAGGACTCTCATCCCATCCACCTTCTTCAGGGACTTCGCCATCA; GCGTCGTGGTGAAGCCCAGCAGCACCCGTGGTGGCGTGCTCTTCGCCATCACTGACGCCTTCCAGAAGGT; CATCTACCTGGGCCTGCGGCTCTCAGGTGTGGAGGACGGCCACCAGCGGATCATCCTCTACTACACGGAG; CCAGGCTCCCATGTGTCCCAAGAGGCTGCTGCCTTCTCGGTGCCTGTGATGACCCACAGGTGGAACCGCT; TCGCCATGATTGTCCAGGGTGAGGAAGTGACCCTCCTCGTGAACTGTGAGGAGCACAGCCGCATCCCCTT; CCAGCGGTCCTCCCAGGCTTTGGCTTTTGAGTCCAGCGCTGGAATCTTCATGGGCAATGCAGGAGCTACA; GGGCTCGAGAGATTCACTGGCTCCCTCCAGCAGCTCACCGTGCACCCCGACCCCAGGACTCCCGAGGAGC; TGTGTGACCCTGAAGAGTCCTCGGCATCTGGAGAGACCAGTGGGCTGCAGGAGGCAGACGGAGTAGCTGA; GATCTTAGAAGCCGTCACCTACACTCAAGCCTCGCCCAAAGAAGCAAAAGTTGAACCCATAAACACACCT; CCAACTCCATCCTCCCCCTTTGAAGACATGGAACTTTCTGGTGAACCTGTACCCGAGGGGACCCTGGAAA; CCACCAACATGAGCATCATCCAGCACAGCAGCCCCAAACAAGGGTCTGGTGAGATCCTGAATGACACACT; GGAGGGGGTTCATTCTGTGGATGGTGACCCCATTACTGACAGCGGCTCAGGGGCTGGGGCCTTCCTTGAC; ATTGCTGAAGAAAAGAATTTAGCAGCAACAGCAGCGGGGCTGGCCGAGGTGCCCATCAGCACTGCTGGAG; AAGCAGAGGCCAGCAGTGTGCCCACCGGGGGACCAACCCTCTCTATGTCCACGGAGAACCCAGAGGAAGG; GGTCACTCCAGGTCCAGATAATGAAGAGCGTTTAGCAGCAACAGCAGCAGGGGAGGCCGAGGCACTCGCC; AGCATGCCTGGGGAAGTGGAGGCCAGTGGTGTGGCCCCCGGGGAGCTGGACCTCTCCATGTCCGCCCAGA; GCCTCGGGGAAGAGGCCACTGTGGGTCCAAGCAGTGAAGACAGTTTAACAACAGCTGCAGCTGCAACCGA; AGTGTCCCTCAGTACTTTTGAGGATGAGGAAGCCAGTGGGGTCCCCACAGATGGCCTGGCTCCCCTCACA; GCCACCATGGCCCCTGAGCGGGCAGTCACTTCTGGTCCTGGTGATGAAGAAGACTTGGCAGCAGCCACAA; CAGAGGAGCCCCTCATCACAGCTGGGGGTGAAGAGTCCGGCAGCCCTCCCCCTGATGGGCCACCGCTGCC; CCTGCCCACAGTGGCTCCTGAAAGATGGATCACTCCAGCTCAAAGAGAACATGTGGGAATGAAAGGACAG; GCTGGGCCCAAAGGAGAAAAGGGTGATGCTGGGGAGGAGCTTCCTGGCCCTCCTGAACCTTCTGGGCCTG; TTGGACCCACGGCAGGAGCAGAAGCAGAGGGCTCTGGCCTAGGCTGGGGCTCGGACGTCGGCTCTGGCTC; TGGTGACCTGGTGGGCAGTGAGCAGCTGCTGAGAGGTCCTCCAGGACCCCCAGGGCCACCTGGCTTACCT; GGGATTCCAGGAAAACCAGGAACTGATGTTTTCATGGGACCCCCTGGATCTCCTGGAGAGGATGGACCTG; CTGGTGAACCTGGGCCCCCGGGCCCTGAGGGACAGCCTGGAGTTGATGGAGCCACCGGCCTTCCCGGGAT; GAAAGGGGAGAAGGGAGCAAGAGGGCCTAATGGCTCAGTTGGTGAAAAGGGTGACCCTGGCAACAGAGGC; TTACCTGGACCCCCGGGGAAAAAGGGACAAGCTGGCCCTCCTGGGGTCATGGGACCCCCAGGGCCTCCTG; GACCCCCTGGGCCCCCAGGCCCTGGATGCACAATGGGACTTGGATTCGAGGATACCGAAGGCTCTGGAAG; CACCCAGCTATTGAATGAACCCAAACTCTCCAGACCAACGGCTGCAATTGGTCTCAAAGGAGAGAAAGGA; GACCGGGGACCCAAGGGAGAAAGGGGGATGGATGGAGCCAGTATTGTGGGACCCCCTGGGCCGAGAGGGC; CACCTGGGCACATCAAGGTCTTGTCTAATTCCTTGATCAATATCACCCATGGATTCATGAATTTCTCGGA; CATTCCTGAGCTGGTGGGGCCTCCGGGGCCGGACGGGTTGCCTGGGCTGCCAGGATTTCCAGGTCCTAGA; GGACCAAAAGGTGACACTGGTTTACCTGGCTTTCCAGGACTAAAAGGAGAACAGGGCGAGAAGGGAGAGC; CGGGTGCCATCCTGACAGAGGACATTCCTCTGGAAAGGCTGATGGGGAAAAAGGGTGAACCTGGAATGCA; TGGAGCCCCAGGACCAATGGGGCCCAAAGGACCACCAGGACATAAAGGAGAATTTGGCCTTCCCGGGCGA; CCTGGTCGCCCAGGACTGAATGGCCTCAAGGGTACCAAAGGAGATCCAGGGGTCATTATGCAGGGCCCAC; CTGGCTTACCTGGCCCTCCAGGCCCCCCTGGGCCACCTGGAGCTGTGATTAACATCAAAGGAGCCATTTT; CCCAATACCCGTCCGACCACACTGCAAAATGCCAGTTGATACTGCTCATCCTGGGAGTCCAGAGCTCATC; ACTTTTCACGGTGTTAAAGGAGAGAAAGGATCCTGGGGTCTTCCTGGCTCAAAGGGAGAAAAAGGCGACC; AGGGAGCCCAGGGACCACCAGGTCCTCCACTTGATCTAGCTTACCTGAGACACTTTCTGAACAACTTGAA; GGGGGAGAATGGAGACAAGGGGTTCAAAGGTGAAAAAGGAGAAAAAGGAGACATTAATGGCAGCTTCCTT; ATGTCTGGGCCTCCAGGCCTGCCCGGAAATCCAGGCCCGGCTGGCCAAAAAGGGGAGACAGTCGTTGGGC; CCCAAGGACCCCCAGGTGCTCCTGGTCTGCCTGGGCCACCTGGCTTTGGAAGACCTGGTGATCCTGGGCC; ACCGGGGCCCCCGGGGCCACCAGGACCTCCAGCTATCCTGGGAGCAGCTGTGGCCCTTCCAGGTCCCCCT; GGCCCTCCAGGACAGCCAGGGCTTCCCGGATCCAGAAACCTGGTCACAGCATTCAGCAACATGGATGACA; TGCTGCAGAAAGCGCATTTGGTTATAGAAGGAACATTCATCTACCTGAGGGACAGCACTGAGTTTTTCAT; TCGTGTTAGAGATGGCTGGAAAAAATTACAGCTGGGAGAACTGATCCCCATTCCTGCCGACAGCCCTCCA; CCCCCTGCGCTTTCCAGCAACCCACATCAGCTTCTGCCTCCACCAAACCCTATTTCAAGTGCCAATTATG; AGAAGCCTGCTCTGCATTTGGCTGCTCTGAACATGCCATTTTCTGGGGACATTCGAGCTGATTTTCAGTG; CTTCAAGCAGGCCAGAGCTGCAGGACTGTTGTCCACCTACCGAGCATTCTTATCTTCCCATTTGCAAGAT; CTGTCCACCATTGTGAGGAAAGCAGAGAGATACAGCCTTCCCATAGTGAACCTCAAGGGCCAAGTACTTT; TTAATAATTGGGACTCAATTTTTTCTGGCCACGGAGGTCAGTTCAATATGCATATTCCAATATACTCCTT; TGATGGTCGAGACATAATGACAGATCCTTCTTGGCCCCAGAAAGTCATTTGGCATGGCTCCAGCCCCCAT; GGCGTCCGCCTTGTGGATAACTACTGTGAAGCATGGCGAACCGCGGACACAGCGGTCACGGGACTTGCCT; CCCCGCTGAGCACGGGGAAGATTCTGGACCAGAAAGCATACAGCTGTGCTAATCGGCTAATTGTCCTATG; TATCGAAAACAGTTTCATGACAGACGCTAGGAAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215981 representing NM_001855; Red=Cloning site Green=Tags(s); ; MAPRRNNGQCWCLLMLLSVSTPLPAVTQTRGATETASQGHLDLTQLIGVPLPSSVSFVTGYGGFPAYSFG; PGANVGRPARTLIPSTFFRDFAISVVVKPSSTRGGVLFAITDAFQKVIYLGLRLSGVEDGHQRIILYYTE; PGSHVSQEAAAFSVPVMTHRWNRFAMIVQGEEVTLLVNCEEHSRIPFQRSSQALAFESSAGIFMGNAGAT; GLERFTGSLQQLTVHPDPRTPEELCDPEESSASGETSGLQEADGVAEILEAVTYTQASPKEAKVEPINTP; PTPSSPFEDMELSGEPVPEGTLETTNMSIIQHSSPKQGSGEILNDTLEGVHSVDGDPITDSGSGAGAFLD; IAEEKNLAATAAGLAEVPISTAGEAEASSVPTGGPTLSMSTENPEEGVTPGPDNEERLAATAAGEAEALA; SMPGEVEASGVAPGELDLSMSAQSLGEEATVGPSSEDSLTTAAAATEVSLSTFEDEEASGVPTDGLAPLT; ATMAPERAVTSGPGDEEDLAAATTEEPLITAGGEESGSPPPDGPPLPLPTVAPERWITPAQREHVGMKGQ; AGPKGEKGDAGEELPGPPEPSGPVGPTAGAEAEGSGLGWGSDVGSGSGDLVGSEQLLRGPPGPPGPPGLP; GIPGKPGTDVFMGPPGSPGEDGPAGEPGPPGPEGQPGVDGATGLPGMKGEKGARGPNGSVGEKGDPGNRG; LPGPPGKKGQAGPPGVMGPPGPPGPPGPPGPGCTMGLGFEDTEGSGSTQLLNEPKLSRPTAAIGLKGEKG; DRGPKGERGMDGASIVGPPGPRGPPGHIKVLSNSLINITHGFMNFSDIPELVGPPGPDGLPGLPGFPGPR; GPKGDTGLPGFPGLKGEQGEKGEPGAILTEDIPLERLMGKKGEPGMHGAPGPMGPKGPPGHKGEFGLPGR; PGRPGLNGLKGTKGDPGVIMQGPPGLPGPPGPPGPPGAVINIKGAIFPIPVRPHCKMPVDTAHPGSPELI; TFHGVKGEKGSWGLPGSKGEKGDQGAQGPPGPPLDLAYLRHFLNNLKGENGDKGFKGEKGEKGDINGSFL; MSGPPGLPGNPGPAGQKGETVVGPQGPPGAPGLPGPPGFGRPGDPGPPGPPGPPGPPAILGAAVALPGPP; GPPGQPGLPGSRNLVTAFSNMDDMLQKAHLVIEGTFIYLRDSTEFFIRVRDGWKKLQLGELIPIPADSPP; PPALSSNPHQLLPPPNPISSANYEKPALHLAALNMPFSGDIRADFQCFKQARAAGLLSTYRAFLSSHLQD; LSTIVRKAERYSLPIVNLKGQVLFNNWDSIFSGHGGQFNMHIPIYSFDGRDIMTDPSWPQKVIWHGSSPH; GVRLVDNYCEAWRTADTAVTGLASPLSTGKILDQKAYSCANRLIVLCIENSFMTDARK; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001855
• ORF Size: 4164 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001855.5
• RefSeq Size: 5222 bp
• RefSeq ORF: 4167 bp
• Locus ID: 1306
• UniProt ID: P39059
• Cytogenetics: 9q22.33
• MW: 141.72 kDa
• Summary: This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration. [provided by RefSeq, May 2013]