Collagen XV (COL15A1) (NM_001855) Human Untagged Clone

SKU SC303085

COL15A1 (untagged)-Human collagen, type XV, alpha 1 (COL15A1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Collagen XV
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303085 representing NM_001855.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCACCAAGGAGGAACAACGGGCAGTGCTGGTGTCTGCTGATGCTGCTCTCGGTCTCCACGCCCCTC; CCTGCTGTCACCCAGACCCGCGGTGCGACAGAGACTGCTTCCCAGGGTCACCTGGACCTCACGCAGCTC; ATCGGTGTCCCGCTGCCCTCGTCCGTATCCTTTGTCACAGGCTATGGTGGCTTCCCGGCCTACAGTTTC; GGGCCTGGTGCCAATGTTGGCCGCCCAGCCAGGACTCTCATCCCATCCACCTTCTTCAGGGACTTCGCC; ATCAGCGTCGTGGTGAAGCCCAGCAGCACCCGTGGTGGCGTGCTCTTCGCCATCACTGACGCCTTCCAG; AAGGTCATCTACCTGGGCCTGCGGCTCTCAGGTGTGGAGGACGGCCACCAGCGGATCATCCTCTACTAC; ACGGAGCCAGGCTCCCATGTGTCCCAAGAGGCTGCTGCCTTCTCGGTGCCTGTGATGACCCACAGGTGG; AACCGCTTCGCCATGATTGTCCAGGGTGAGGAAGTGACCCTCCTCGTGAACTGTGAGGAGCACAGCCGC; ATCCCCTTCCAGCGGTCCTCCCAGGCTTTGGCTTTTGAGTCCAGCGCTGGAATCTTCATGGGCAATGCA; GGAGCTACAGGGCTCGAGAGATTCACTGGCTCCCTCCAGCAGCTCACCGTGCACCCCGACCCCAGGACT; CCCGAGGAGCTGTGTGACCCTGAAGAGTCCTCGGCATCTGGAGAGACCAGTGGGCTGCAGGAGGCAGAC; GGAGTAGCTGAGATCTTAGAAGCCGTCACCTACACTCAAGCCTCGCCCAAAGAAGCAAAAGTTGAACCC; ATAAACACACCTCCAACTCCATCCTCCCCCTTTGAAGACATGGAACTTTCTGGTGAACCTGTACCCGAG; GGGACCCTGGAAACCACCAACATGAGCATCATCCAGCACAGCAGCCCCAAACAAGGGTCTGGTGAGATC; CTGAATGACACACTGGAGGGGGTTCATTCTGTGGATGGTGACCCCATTACTGACAGCGGCTCAGGGGCT; GGGGCCTTCCTTGACATTGCTGAAGAAAAGAATTTAGCAGCAACAGCAGCGGGGCTGGCCGAGGTGCCC; ATCAGCACTGCTGGAGAAGCAGAGGCCAGCAGTGTGCCCACCGGGGGACCAACCCTCTCTATGTCCACG; GAGAACCCAGAGGAAGGGGTCACTCCAGGTCCAGATAATGAAGAGCGTTTAGCAGCAACAGCAGCAGGG; GAGGCCGAGGCACTCGCCAGCATGCCTGGGGAAGTGGAGGCCAGTGGTGTGGCCCCCGGGGAGCTGGAC; CTCTCCATGTCCGCCCAGAGCCTCGGGGAAGAGGCCACTGTGGGTCCAAGCAGTGAAGACAGTTTAACA; ACAGCTGCAGCTGCAACCGAAGTGTCCCTCAGTACTTTTGAGGATGAGGAAGCCAGTGGGGTCCCCACA; GATGGCCTGGCTCCCCTCACAGCCACCATGGCCCCTGAGCGGGCAGTCACTTCTGGTCCTGGTGATGAA; GAAGACTTGGCAGCAGCCACAACAGAGGAGCCCCTCATCACAGCTGGGGGTGAAGAGTCCGGCAGCCCT; CCCCCTGATGGGCCACCGCTGCCCCTGCCCACAGTGGCTCCTGAAAGATGGATCACTCCAGCTCAAAGA; GAACATGTGGGAATGAAAGGACAGGCTGGGCCCAAAGGAGAAAAGGGTGATGCTGGGGAGGAGCTTCCT; GGCCCTCCTGAACCTTCTGGGCCTGTTGGACCCACGGCAGGAGCAGAAGCAGAGGGCTCTGGCCTAGGC; TGGGGCTCGGACGTCGGCTCTGGCTCTGGTGACCTGGTGGGCAGTGAGCAGCTGCTGAGAGGTCCTCCA; GGACCCCCAGGGCCACCTGGCTTACCTGGGATTCCAGGAAAACCAGGAACTGATGTTTTCATGGGACCC; CCTGGATCTCCTGGAGAGGATGGACCTGCTGGTGAACCTGGGCCCCCGGGCCCTGAGGGACAGCCTGGA; GTTGATGGAGCCACCGGCCTTCCCGGGATGAAAGGGGAGAAGGGAGCAAGAGGGCCTAATGGCTCAGTT; GGTGAAAAGGGTGACCCTGGCAACAGAGGCTTACCTGGACCCCCGGGGAAAAAGGGACAAGCTGGCCCT; CCTGGGGTCATGGGACCCCCAGGGCCTCCTGGACCCCCTGGGCCCCCAGGCCCTGGATGCACAATGGGA; CTTGGATTCGAGGATACCGAAGGCTCTGGAAGCACCCAGCTATTGAATGAACCCAAACTCTCCAGACCA; ACGGCTGCAATTGGTCTCAAAGGAGAGAAAGGAGACCGGGGACCCAAGGGAGAAAGGGGGATGGATGGA; GCCAGTATTGTGGGACCCCCTGGGCCGAGAGGGCCACCTGGGCACATCAAGGTCTTGTCTAATTCCTTG; ATCAATATCACCCATGGATTCATGAATTTCTCGGACATTCCTGAGCTGGTGGGGCCTCCGGGGCCGGAC; GGGTTGCCTGGGCTGCCAGGATTTCCAGGTCCTAGAGGACCAAAAGGTGACACTGGTTTACCTGGCTTT; CCAGGACTAAAAGGAGAACAGGGCGAGAAGGGAGAGCCGGGTGCCATCCTGACAGAGGACATTCCTCTG; GAAAGGCTGATGGGGAAAAAGGGTGAACCTGGAATGCATGGAGCCCCAGGACCAATGGGGCCCAAAGGA; CCACCAGGACATAAAGGAGAATTTGGCCTTCCCGGGCGACCTGGTCGCCCAGGACTGAATGGCCTCAAG; GGTACCAAAGGAGATCCAGGGGTCATTATGCAGGGCCCACCTGGCTTACCTGGCCCTCCAGGCCCCCCT; GGGCCACCTGGAGCTGTGATTAACATCAAAGGAGCCATTTTCCCAATACCCGTCCGACCACACTGCAAA; ATGCCAGTTGATACTGCTCATCCTGGGAGTCCAGAGCTCATCACTTTTCACGGTGTTAAAGGAGAGAAA; GGATCCTGGGGTCTTCCTGGCTCAAAGGGAGAAAAAGGCGACCAGGGAGCCCAGGGACCACCAGGTCCT; CCACTTGATCTAGCTTACCTGAGACACTTTCTGAACAACTTGAAGGGGGAGAATGGAGACAAGGGGTTC; AAAGGTGAAAAAGGAGAAAAAGGAGACATTAATGGCAGCTTCCTTATGTCTGGGCCTCCAGGCCTGCCC; GGAAATCCAGGCCCGGCTGGCCAAAAAGGGGAGACAGTCGTTGGGCCCCAAGGACCCCCAGGTGCTCCT; GGTCTGCCTGGGCCACCTGGCTTTGGAAGACCTGGTGATCCTGGGCCACCGGGGCCCCCGGGGCCACCA; GGACCTCCAGCTATCCTGGGAGCAGCTGTGGCCCTTCCAGGTCCCCCTGGCCCTCCAGGACAGCCAGGG; CTTCCCGGATCCAGAAACCTGGTCACAGCATTCAGCAACATGGATGACATGCTGCAGAAAGCGCATTTG; GTTATAGAAGGAACATTCATCTACCTGAGGGACAGCACTGAGTTTTTCATTCGTGTTAGAGATGGCTGG; AAAAAATTACAGCTGGGAGAACTGATCCCCATTCCTGCCGACAGCCCTCCACCCCCTGCGCTTTCCAGC; AACCCACATCAGCTTCTGCCTCCACCAAACCCTATTTCAAGTGCCAATTATGAGAAGCCTGCTCTGCAT; TTGGCTGCTCTGAACATGCCATTTTCTGGGGACATTCGAGCTGATTTTCAGTGCTTCAAGCAGGCCAGA; GCTGCAGGACTGTTGTCCACCTACCGAGCATTCTTATCTTCCCATTTGCAAGATCTGTCCACCATTGTG; AGGAAAGCAGAGAGATACAGCCTTCCCATAGTGAACCTCAAGGGCCAAGTACTTTTTAATAATTGGGAC; TCAATTTTTTCTGGCCACGGAGGTCAGTTCAATATGCATATTCCAATATACTCCTTTGATGGTCGAGAC; ATAATGACAGATCCTTCTTGGCCCCAGAAAGTCATTTGGCATGGCTCCAGCCCCCATGGCGTCCGCCTT; GTGGATAACTACTGTGAAGCATGGCGAACCGCGGACACAGCGGTCACGGGACTTGCCTCCCCGCTGAGC; ACGGGGAAGATTCTGGACCAGAAAGCATACAGCTGTGCTAATCGGCTAATTGTCCTATGTATCGAAAAC; AGTTTCATGACAGACGCTAGGAAGTAA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001855
• Insert Size: 4167 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001855.4
• RefSeq Size: 5422 bp
• RefSeq ORF: 4167 bp
• Locus ID: 1306
• UniProt ID: P39059
• Cytogenetics: 9q22.33
• MW: 141.7 kDa
• Summary: This gene encodes the alpha chain of type XV collagen, a member of the FACIT collagen family (fibril-associated collagens with interrupted helices). Type XV collagen has a wide tissue distribution but the strongest expression is localized to basement membrane zones so it may function to adhere basement membranes to underlying connective tissue stroma. The proteolytically produced C-terminal fragment of type XV collagen is restin, a potentially antiangiogenic protein that is closely related to endostatin. Mouse studies have shown that collagen XV deficiency is associated with muscle and microvessel deterioration. [provided by RefSeq, May 2013]