COL21A1 (NM_030820) Human Tagged ORF Clone

SKU
RC215724
COL21A1 (Myc-DDK-tagged)-Human collagen, type XXI, alpha 1 (COL21A1)
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol COL21A1
Synonyms COLA1L; FP633
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC215724 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCTCACTATATTACATTTCTCTGCATGGTTTTGGTGCTGCTTCTTCAGAATTCTGTGTTAGCTGAAG
ATGGGGAAGTAAGATCAAGTTGTCGTACTGCTCCGACAGATTTAGTTTTCATCTTAGATGGCTCTTATAG
TGTTGGCCCAGAAAACTTTGAAATAGTGAAAAAGTGGCTTGTCAATATCACAAAAAACTTTGACATAGGG
CCGAAGTTTATTCAAGTTGGAGTGGTTCAATATAGTGACTACCCTGTGCTGGAGATTCCTCTCGGAAGCT
ATGATTCAGGAGAACATTTGACGGCAGCAGTGGAATCCATACTCTACTTAGGAGGAAACACAAAGACAGG
GAAGGCCATCCAGTTTGCGCTCGATTACCTTTTTGCCAAGTCCTCACGATTTCTGACTAAGATAGCAGTG
GTACTTACGGATGGCAAGTCCCAAGATGACGTCAAGGATGCAGCTCAAGCAGCAAGAGATAGTAAGATAA
CATTATTTGCTATTGGTGTTGGTTCAGAAACAGAAGATGCCGAACTTAGAGCTATTGCCAACAAGCCTTC
GTCTACTTATGTGTTTTATGTGGAAGACTATATTGCAATATCCAAAATAAGGGAAGTGATGAAGCAGAAA
CTTTGTGAAGAATCTGTCTGTCCAACACGAATTCCAGTGGCAGCTCGTGATGAAAGGGGATTTGATATTC
TTTTAGGTTTAGATGTAAATAAAAAGGTTAAGAAAAGAATACAGCTTTCACCAAAAAAGATAAAAGGATA
TGAAGTAACATCAAAAGTTGATTTATCAGAACTCACAAGCAATGTTTTCCCAGAAGGTCTTCCTCCATCA
TATGTATTTGTGTCTACTCAAAGATTTAAAGTCAAGAAAATTTGGGATTTATGGAGAATATTAACTATTG
ATGGAAGGCCACAAATAGCAGTTACCTTAAATGGTGTGGACAAAATCTTATTATTTACAACAACCAGCGT
AATTAATGGCTCACAAGTGGTTACCTTTGCTAACCCTCAAGTTAAGACGTTGTTTGATGAAGGCTGGCAC
CAAATTCGTCTCTTAGTAACAGAACAAGATGTGACTTTGTATATTGATGACCAACAAATTGAAAACAAGC
CCTTACATCCAGTTTTAGGGATCTTGATCAATGGGCAAACCCAAATTGGAAAATATTCTGGAAAAGAAGA
AACTGTTCAGTTTGATGTCCAAAAGTTGCGAATCTACTGTGACCCAGAACAGAACAACCGGGAGACAGCA
TGTGAGATTCCTGGATTTAATGGAGAGTGCCTTAATGGTCCCAGTGATGTAGGTTCAACTCCAGCTCCCT
GTATTTGTCCTCCGGGAAAACCAGGACTTCAAGGCCCCAAAGGTGACCCTGGACTGCCTGGGAACCCTGG
CTACCCTGGACAACCTGGTCAAGATGGTAAGCCTGGATATCAGGGAATTGCAGGGACACCAGGTGTTCCA
GGATCTCCAGGAATACAAGGAGCTCGAGGACTACCAGGTTACAAAGGAGAACCAGGGCGAGATGGTGACA
AGGGTGATCGTGGACTTCCTGGTTTTCCTGGGCTTCATGGCATGCCAGGATCAAAGGGTGAAATGGGTGC
CAAAGGAGACAAAGGATCACCTGGATTTTATGGCAAAAAGGGTGCAAAAGGTGAAAAGGGGAATGCTGGC
TTCCCTGGCCTCCCTGGACCTGCTGGAGAACCAGGAAGACATGGAAAGGATGGATTAATGGGTAGTCCCG
GTTTCAAGGGAGAAGCAGGATCCCCTGGTGCTCCGGGGCAGGATGGAACACGGGGAGAGCCTGGAATCCC
AGGATTTCCTGGAAACCGAGGATTAATGGGCCAAAAGGGAGAAATTGGGCCTCCAGGACAGCAAGGAAAA
AAAGGAGCCCCAGGGATGCCTGGTTTAATGGGAAGCAATGGCTCACCAGGCCAGCCTGGAACACCGGGAT
CTAAGGGAAGCAAAGGTGAACCTGGAATTCAAGGGATGCCTGGGGCTTCTGGGCTCAAGGGAGAACCAGG
AGCAACGGGTTCCCCAGGAGAACCAGGATACATGGGTTTACCCGGGATTCAAGGAAAAAAGGGGGACAAA
GGAAATCAAGGTGAAAAAGGTATTCAGGGTCAAAAGGGAGAAAATGGAAGACAGGGAATTCCAGGGCAAC
AGGGAATTCAAGGCCATCATGGTGCAAAAGGAGAGAGAGGTGAAAAGGGAGAACCTGGTGTCCGAGGTGC
CATTGGATCAAAAGGAGAATCTGGGGTGGATGGCTTGATGGGGCCCGCAGGTCCTAAGGGGCAACCTGGG
GATCCAGGTCCTCAGGGACCCCCAGGTTTGGATGGGAAGCCCGGAAGAGAGTTTTCAGAACAATTTATTC
GACAAGTTTGCACAGATGTAATAAGAGCCCAGCTACCAGTCTTACTTCAGAGTGGAAGAATTAGAAATTG
TGATCATTGCCTGTCCCAACATGGCTCCCCGGGTATTCCTGGGCCACCTGGTCCGATAGGCCCAGAGGGT
CCCAGAGGATTACCTGGTTTGCCAGGAAGAGATGGTGTTCCTGGATTAGTGGGTGTCCCTGGACGTCCAG
GTGTCAGAGGATTAAAAGGCCTACCAGGAAGAAATGGGGAAAAAGGGAGCCAAGGGTTTGGGTATCCTGG
AGAACAAGGTCCTCCTGGTCCCCCAGGTCCAGAGGGCCCTCCTGGAATAAGCAAAGAAGGTCCTCCAGGA
GACCCAGGTCTCCCTGGCAAAGATGGAGACCATGGAAAACCTGGAATCCAAGGGCAACCAGGCCCCCCAG
GCATCTGCGACCCATCACTATGTTTTAGTGTAATTGCCAGAAGAGATCCGTTCAGAAAAGGACCAAACTA
T


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC215724 protein sequence
Red=Cloning site Green=Tags(s)

MAHYITFLCMVLVLLLQNSVLAEDGEVRSSCRTAPTDLVFILDGSYSVGPENFEIVKKWLVNITKNFDIG
PKFIQVGVVQYSDYPVLEIPLGSYDSGEHLTAAVESILYLGGNTKTGKAIQFALDYLFAKSSRFLTKIAV
VLTDGKSQDDVKDAAQAARDSKITLFAIGVGSETEDAELRAIANKPSSTYVFYVEDYIAISKIREVMKQK
LCEESVCPTRIPVAARDERGFDILLGLDVNKKVKKRIQLSPKKIKGYEVTSKVDLSELTSNVFPEGLPPS
YVFVSTQRFKVKKIWDLWRILTIDGRPQIAVTLNGVDKILLFTTTSVINGSQVVTFANPQVKTLFDEGWH
QIRLLVTEQDVTLYIDDQQIENKPLHPVLGILINGQTQIGKYSGKEETVQFDVQKLRIYCDPEQNNRETA
CEIPGFNGECLNGPSDVGSTPAPCICPPGKPGLQGPKGDPGLPGNPGYPGQPGQDGKPGYQGIAGTPGVP
GSPGIQGARGLPGYKGEPGRDGDKGDRGLPGFPGLHGMPGSKGEMGAKGDKGSPGFYGKKGAKGEKGNAG
FPGLPGPAGEPGRHGKDGLMGSPGFKGEAGSPGAPGQDGTRGEPGIPGFPGNRGLMGQKGEIGPPGQQGK
KGAPGMPGLMGSNGSPGQPGTPGSKGSKGEPGIQGMPGASGLKGEPGATGSPGEPGYMGLPGIQGKKGDK
GNQGEKGIQGQKGENGRQGIPGQQGIQGHHGAKGERGEKGEPGVRGAIGSKGESGVDGLMGPAGPKGQPG
DPGPQGPPGLDGKPGREFSEQFIRQVCTDVIRAQLPVLLQSGRIRNCDHCLSQHGSPGIPGPPGPIGPEG
PRGLPGLPGRDGVPGLVGVPGRPGVRGLKGLPGRNGEKGSQGFGYPGEQGPPGPPGPEGPPGISKEGPPG
DPGLPGKDGDHGKPGIQGQPGPPGICDPSLCFSVIARRDPFRKGPNY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_030820
ORF Size 2871 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_030820.4
RefSeq Size 4193 bp
RefSeq ORF 2874 bp
Locus ID 81578
UniProt ID Q96P44
Cytogenetics 6p12.3-p11.2
Domains Collagen, TSPN, VWA
MW 99.4 kDa
Summary This gene encodes the alpha chain of type XXI collagen, a member of the FACIT (fibril-associated collagens with interrupted helices) collagen family. Type XXI collagen is localized to tissues containing type I collagen and maintains the integrity of the extracellular matrix. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.