COL21A1 (NM_030820) Human Untagged Clone

SKU SC109391

COL21A1 (untagged)-Human collagen, type XXI, alpha 1 (COL21A1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: COL21A1
• Synonyms: COLA1L; FP633
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC109391 sequence for NM_030820 edited (data generated by NextGen Sequencing); ATGGCTCACTATATTACATTTCTCTGCATGGTTTTGGTGCTGCTTCTTCAGAATTCTGTG; TTAGCTGAAGATGGGGAAGTAAGATCAAGTTGTCGTACTGCTCCGACAGATTTAGTTTTC; ATCTTAGATGGCTCTTATAGTGTTGGCCCAGAAAACTTTGAAATAGTGAAAAAGTGGCTT; GTCAATATCACAAAAAACTTTGACATAGGGCCGAAGTTTATTCAAGTTGGAGTGGTTCAA; TATAGTGACTACCCTGTGCTGGAGATTCCTCTCGGAAGCTATGATTCAGGAGAACATTTG; ACGGCAGCAGTGGAATCCATACTCTACTTAGGAGGAAACACAAAGACAGGGAAGGCCATC; CAGTTTGCGCTCGATTACCTTTTTGCCAAGTCCTCACGATTTCTGACTAAGATAGCAGTG; GTACTTACGGATGGCAAGTCCCAAGATGACGTCAAGGATGCAGCTCAAGCAGCAAGAGAT; AGTAAGATAACATTATTTGCTATTGGTGTTGGTTCAGAAACAGAAGATGCCGAACTTAGA; GCTATTGCCAACAAGCCTTCGTCTACTTATGTGTTTTATGTGGAAGACTATATTGCAATA; TCCAAAATAAGGGAAGTGATGAAGCAGAAACTTTGTGAAGAATCTGTCTGTCCAACACGA; ATTCCAGTGGCAGCTCGTGATGAAAGGGGATTTGATATTCTTTTAGGTTTAGATGTAAAT; AAAAAGGTTAAGAAAAGAATACAGCTTTCACCAAAAAAGATAAAAGGATATGAAGTAACA; TCAAAAGTTGATTTATCAGAACTCACAAGCAATGTTTTCCCAGAAGGTCTTCCTCCATCA; TATGTATTTGTGTCTACTCAAAGATTTAAAGTCAAGAAAATTTGGGATTTATGGAGAATA; TTAACTATTGATGGAAGGCCACAAATAGCAGTTACCTTAAATGGTGTGGACAAAATCTTA; TTATTTACAACAACCAGCGTAATTAATGGCTCACAAGTGGTTACCTTTGCTAACCCTCAA; GTTAAGACGTTGTTTGATGAAGGCTGGCACCAAATTCGTCTCTTAGTAACAGAACAAGAT; GTGACTTTGTATATTGATGACCAACAAATTGAAAACAAGCCCTTACATCCAGTTTTAGGG; ATCTTGATCAATGGGCAAACCCAAATTGGAAAATATTCTGGAAAAGAAGAAACTGTTCAG; TTTGATGTCCAAAAGTTGCGAATCTACTGTGACCCAGAACAGAACAACCGGGAGACAGCA; TGTGAGATTCCTGGATTTAATGGAGAGTGCCTTAATGGTCCCAGTGATGTAGGTTCAACT; CCAGCTCCCTGTATTTGTCCTCCGGGAAAACCAGGACTTCAAGGCCCCAAAGGTGACCCT; GGACTGCCTGGGAACCCTGGCTACCCTGGACAACCTGGTCAAGATGGNNNNNNNNNATAT; CAGGGAATTGCAGGGACACCAGGTGTTCCAGGATCTCCAGGAATACAAGGAGCTCGAGGA; CTACCAGGTTACAAAGGAGAACCAGGGCGAGATGGTGACAAGGGTGATCGTGGACTTCCT; GGTTTTCCTGGGCTTCATGGCATGCCAGGATCAAAGGGTGAAATGGGTGCCAAAGGAGAC; AAAGGATCACCTGGATTTTATGGCAAAAAGGGTGCAAAAGGTGAAAAGGGGAATGCTGGC; TTCCCTGGCCTCCCTGGACCTGCTGGAGAACCAGGAAGACATGGAAAGGATGGATTAATG; GGTAGTCCCGGTTNNAAGGGAGAAGCAGGATCCCCTGGTGCTCCGGGGCAGGATGGAACA; CGGGGAGAGCCTGGAATCCCAGGATTTCCTGGAAACCGAGGATTAATGGGCCAAAAGGGA; GAAATTGGGCCTCCAGGACAGCAAGGAAAAAAAGGAGCCCCAGGGATGCCTGGTTTAATG; GGAAGCAATGGCTCACCAGGCCAGCCTGGAACACCGGGATCTAAGGGAAGCAAAGGTGAA; CCTGGAATTCAAGGGATGCCTGGGGCTTCTGGGCTCAAGGGAGAACCAGGAGCAACGGGT; TCCCCAGGAGAACCAGGATACATGGGTTTACCCGGGATTCAAGGAAAAAAGGGGGACAAA; GGAAATCAAGGTGAAAAAGGTATTCAGGGTCAAAAGGGAGAAAATGGAAGACAGGGAATT; CCAGGGCAACAGGGAATTCAAGGCCATCATGGTGCAAAAGGAGAGAGAGGTGAAAAGGGA; GAACCTGGTGTCCGAGGTGCCATTGGATCAAAAGGAGAATCTGGGGTGGATGGCTTGATG; GGGCCCGCAGGTCCTAAGGGGCAACCTGGGGATCCAGGTCCTCAGGGACCCCCAGGTTTG; GATGGGAAGCCCGGAAGAGAGTTTTCAGAACAATTTATTCGACAAGTTTGCACAGATGTA; ATAAGAGCCCAGCTACCAGTCTTACTTCAGAGTGGAAGAATTAGAAATTGTGATCATTGC; CTGTCCCAACATGGCTCCCCGGGTATTCCTGGGCCACCTGGTCCGATAGGCCCAGAGGGT; CCCAGAGGATTACCTGGTTTGCCAGGAAGAGATGGTGTTCCTGGATTAGTGGGTGTCCCT; GGACGTCCAGGTGTCAGAGGATTAAAAGGCCTACCAGGAAGAAATGGGGAAAAAGGGAGC; CAAGGGTTTGGGTATCCTGGAGAACAAGGTCCTCCTGGTCCCCCAGGTCCAGAGGGCCCT; CCTGGAATAAGCAAAGAAGGTCCTCCAGGAGACCCAGGTCTCCCTGGCAAAGATGGAGAC; CATGGAAAACCTGGAATCCAAGGGCAACCAGGCCCCCCAGGCATCTGCGACCCATCACTA; TGTTTTAGTGTAATTGCCAGAAGAGATCCGTTCAGAAAAGGACCAAACTATTAG; ; Clone variation with respect to NM_030820.3; 438 a=>g;1428 t=>n;1429 a=>n;1430 a=>n;1431 g=>n;1432 c=>n;1433 c=>n;1434 t=>n;1435 g=>n;1436 g=>n;1754 t=>n;1755 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_030820 unedited; CACATTTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCAACACTCAGC; TGGCTGCGACGGCAACCCCGAGCCTGGACACTGCGCCAGGAAACCTCTTCTGGCATCCTT; AGTAAATGGCAGTAGAGTCATCTGGGAGGTAAAAGAAGATTGTACCATGTGAGGGATCTC; CTACCCAGGAAACAAAGCCCACAGGAAGACCATCACATAGGAATGGAATCCTAAAACCAA; AATATTAGAACGAAAACAGAAACATGGCTCACTATATTACATTTCTCTGCATGGTTTTGG; TGCTGCTTCTTCAGAATTCTGTGTTAGCTGAAGATGGGGAAGTAAGATCAAGTTGTCGTA; CTGCTCCGACAGATTTAGTTTTCATCTTAGATGGCTCTTATAGTGTTGGCCCAGAAAACT; TTGAAATAGTGAAAAAGTGGCTTGTCAATATCACAAAAAACTTTGACATAGGGCCGAAGT; TTATTCAAGTTGGAGTGGTTCAATATAGTGACTACCCTGTGCTGGAGATTCCTCTCGGAA; GCTATGATTCAGGAGAACATTTGACGGCAGCAGTGGAATCCATACTCTACTTAGGAGGAA; ACACAAAGACAGGGAAGGCCATCCAGTTTGCGCTCGATTACCTTTTTGCCAAGTCCTCAC; GATTTCTGACTAAGATAGCAGTGGTACTTACGGATGGCAAGTCCCAAGATGACGTCAAGG; ATGCAGCTCAAGCAGCAAGAGATAGTAAGATAACATTATTTGCTATTGGNTGNTGGTTCA; GAAACAGAAGATGCCGAACTTAGAGCTATTGCCAACAAGCCTTCGTCTACTTATGTGTTT; TATGGTGGAGACTATATTGCATATCCAAATAAGGNAAGTGATGAGCAGAAACTTGTGAAG; ATCTGTCTGTCCACACGAATTCAGTGCAGCTCGGATGAAGGGGATTGATATCTTTAGN;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_030820 unedited; NAGCTCTGGACCCGCGGCCGCAATCTANGATCGAGNTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTAAAGCCCGGACCAAAATTTTATTATCCTTTGAAAAACAG; GCTGGAAACAAATTTTTGAGCATGACAGGGAGAAAATTTTTAAAATTTGGTTTTTTACAA; AGGCTTTAACCCCAATCCCCAAAGCCGGTTCATAATTTTTAACAGCCAGTTAAAAAAAAC; AAAACTTTCTAGGATTTAAGGAGTTTTCTTTTTTTTTAAACAAAAGCATAACCTTTATTC; CCATTACCTTGGCTCCAGGGAAAATTACCTTTTGGGGCCTTCCCCTTTTAGGGGAAGGGG; AAAAGGAGGGCTTCCAACAAACTTTCCGATAAATAAACAAGTAAAAAAAAGCCTTTAAGA; CCTCCCAAACTAACATTTTTTTTGAAGGACCAAGGAAACTTTTGGCCAAAAAGGTTTTAA; AGGTAAATTTGGACCATCCCCAAAGAAATAAATACTTTTTTTTACCCCCCCCTTTAATCT; ATTTTTAGAGTTGGAAAGGAATAAAAAAACACTTTATTTTTATTCAGAGGGGCTATTCAC; AACACCGACCAACGGGCAATCCACGGCCCTTTATCAGGGTTGAAACACGGAAGAAAAGGA; GTAGTTCACCAATACGGTTTATCAAGGGGAATATACCCCCGGATTTACTTCCAAGGAGGA; GATCCAGGTTTGGGCTTAGGTAGCCCCCGGCCCTTTGAAATGGAAACTTGAAGGCCCCTG; GGCCATGAAATTAACCCCAAACTTTTACTGCCTGGGAGGCTGATGTGAAAAATTTTCCAG; TGCACTAAACGGGCCTGCTTAACGGGCAGGGGGGGATTTTTATTTTCAACTTGGATTAAA; AAAAGGGCCTTTACAGGAACCCTTGGGAATAAATAAGGCGGATAACCGAGGCCTCN;
• Restriction Sites: NotI-NotI
• ACCN: NM_030820
• Insert Size: 4310 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_030820.2, NP_110447.2
• RefSeq Size: 4154 bp
• RefSeq ORF: 2874 bp
• Locus ID: 81578
• UniProt ID: Q96P44
• Cytogenetics: 6p12.3-p11.2
• Domains: Collagen, TSPN, VWA
• Summary: This gene encodes the alpha chain of type XXI collagen, a member of the FACIT (fibril-associated collagens with interrupted helices) collagen family. Type XXI collagen is localized to tissues containing type I collagen and maintains the integrity of the extracellular matrix. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]; Transcript Variant: This variant (1) encodes the longest isoform (a). Variants 1 and 2 encode the same isoform (a).