XPO6 (NM_015171) Human Tagged ORF Clone

SKU
RC214839
XPO6 (Myc-DDK-tagged)-Human exportin 6 (XPO6)
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    Protein expression verified by Western blot, fully sequenced and in stock

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$979.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol XPO6
Synonyms EXP6; RANBP20
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC214839 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCATCTGAAGAAGCCTCTCTCAGGGCATTGGAAAGTCTGATGACAGAATTTTTTCACGATTGTACAA
CCAATGAAAGAAAACGTGAGATAGAGGAGCTTCTTAATAACTTTGCCCAGCAAATAGGAGCCTGGAGATT
CTGCCTGTACTTTCTCTCCAGCACTAGGAATGACTATGTAATGATGTACAGTTTAACAGTTTTTGAGAAT
CTGATCAATAAAATGTGGCTTGGGGTCCCATCTCAGGATAAGATGGAAATCCGTAGCTGTCTGCCCAAAC
TCCTTTTGGCTCACCATAAAACCTTACCTTACTTTATCCGGAACAAGCTCTGCAAAGTTATTGTTGATAT
TGGACGTCAGGATTGGCCCATGTTCTACCACGACTTTTTTACTAACATTTTACAGTTGATCCAGTCCCCT
GTGACAACCCCCCTTGGGCTGATCATGTTGAAGACAACTTCAGAAGAGCTGGCTTGTCCCCGTGAGGACC
TCAGTGTGGCTCGGAAGGAGGAGTTGCGGAAGCTGCTACTGGACCAGGTGCAGACAGTGCTTGGGCTACT
GACAGGTATCTTGGAGACTGTCTGGGACAAACACAGTGTTACTGCTGCCACTCCACCACCATCCCCGACC
TCAGGAGAAAGTGGTGACTTACTGAGTAACCTGTTGCAGAGTCCCAGTTCAGCCAAACTGTTGAATCAGC
CAATTCCCATCCTTGATGTGGAGAGTGAGTATATCTGTTCCCTGGCTTTGGAGTGCCTGGCCCATCTCTT
CAGTTGGATTCCTCTGTCTGCCAGCATCACCCCATCCCTCCTTACCACCATCTTCCACTTTGCACGATTT
GGCTGTGACATCCGGGCCAGAAAGATGGCGTCAGTTAACGGCAGCAGCCAGAACTGTGTCTCGGGTCAGG
AGCGCGGCCGGCTGGGGGTCCTGGCCATGTCCTGCATCAATGAACTCATGTCCAAGAACTGTGTGCCTAT
GGAATTTGAGGAGTATTTACTGCGTATGTTCCAGCAGACTTTCTACCTCCTGCAGAAAATCACCAAGGAT
AACAATGCCCACACAGTGAAGAGCAGGCTAGAAGAGCTCGATGAGAGCTATATCGAGAAGTTTACTGACT
TTCTTCGGCTCTTTGTGAGTGTTCACCTAAGAAGAATCGAGTCTTATTCCCAGTTCCCTGTGGTGGAGTT
TTTGACACTTTTGTTCAAGTACACATTTCATCAGCCTACTCATGAAGGTTACTTCTCTTGTTTGGATATC
TGGACGCTGTTTTTGGACTATCTGACAAGTAAAATTAAAAGTCGTCTTGGAGACAAGGAAGCAGTTCTCA
ACAGGTACGAAGATGCCCTGGTGCTCCTGCTCACAGAGGTGTTGAATCGAATCCAGTTCAGATACAACCA
AGCCCAGCTGGAGGAGTTGGATGATGAGACTCTGGATGACGATCAGCAGACGGAGTGGCAGCGGTACTTA
CGGCAGAGCTTGGAGGTGGTGGCCAAAGTGATGGAGCTCCTGCCCACGCACGCCTTCTCCACACTGTTCC
CTGTTCTTCAGGACAATTTAGAAGTTTATTTGGGATTACAACAGTTTATAGTCACTTCAGGGTCAGGACA
CAGGTTGAACATCACGGCGGAGAACGACTGCCGGCGGCTGCACTGCTCCCTGAGAGACTTGAGCTCCCTG
CTGCAGGCCGTGGGCCGCCTGGCCGAGTACTTTATCGGGGATGTGTTTGCTGCACGGTTCAATGATGCCC
TCACAGTCGTGGAAAGGTTGGTCAAAGTCACTCTGTACGGATCTCAGATAAAATTGTACAACATTGAAAC
TGCTGTGCCATCAGTATTGAAACCTGACCTCATTGATGTGCATGCTCAGTCCCTGGCTGCGCTGCAGGCT
TACTCTCACTGGTTAGCACAGTATTGCAGTGAAGTTCACCGGCAGAACACGCAGCAGTTCGTGACACTCA
TCTCTACTACCATGGATGCAATCACACCTCTAATCAGCACCAAGGTCCAAGACAAGCTGCTGCTATCTGC
GTGCCACTTACTGGTCTCACTGGCCACCACCGTGCGGCCCGTCTTTCTGATCAGCATCCCTGCAGTGCAG
AAAGTATTCAACAGAATCACTGATGCCTCTGCCCTGCGACTTGTCGATAAGGCCCAGGTGTTGGTGTGCC
GAGCCCTCTCTAACATCTTGCTGCTTCCGTGGCCAAACCTTCCAGAGAATGAGCAGCAGTGGCCCGTGCG
CTCCATCAACCATGCCAGCCTCATCTCTGCACTCTCCCGGGACTATCGCAACCTGAAGCCCAGTGCTGTT
GCCCCACAGAGAAAGATGCCACTGGATGACACCAAACTGATTATCCACCAGACACTCAGCGTCTTAGAAG
ATATTGTGGAGAATATCTCGGGGGAGTCCACCAAGTCTCGACAGATTTGCTACCAGTCGCTGCAGGAATC
TGTTCAGGTCTCCCTGGCCCTCTTTCCAGCTTTTATCCATCAGTCAGATGTGACTGATGAGATGCTGAGC
TTCTTCCTCACTCTGTTTCGAGGCCTTAGAGTACAGATGGGTGTGCCTTTCACTGAGCAAATCATACAGA
CTTTCCTCAACATGTTTACCAGAGAGCAGTTAGCCGAGAGCATCCTCCACGAGGGCAGCACAGGCTGCCG
GGTGGTGGAGAAGTTTCTGAAGATCCTGCAGGTGGTGGTCCAGGAGCCAGGCCAGGTGTTCAAGCCCTTC
CTCCCCAGCATCATCGCCCTGTGCATGGAGCAAGTGTATCCCATCATTGCCGAGCGTCCCTCCCCTGATG
TGAAGGCCGAGCTGTTTGAGCTCCTTTTCCGGACGCTCCATCACAACTGGAGGTACTTCTTCAAGTCCAC
CGTGCTGGCCAGTGTCCAGAGGGGGATCGCTGAGGAGCAGATGGAGAATGAGCCCCAGTTCAGTGCCATC
ATGCAGGCTTTCGGACAGTCCTTTCTCCAGCCCGACATCCACCTTTTTAAACAAAATCTCTTCTACTTGG
AGACTCTCAACACCAAGCAGAAGCTGTACCACAAGAAGATCTTCCGGACTGCCATGCTGTTCCAGTTTGT
GAACGTGCTGCTCCAGGTCCTGGTCCACAAGTCCCATGATCTTCTGCAGGAGGAGATTGGCATCGCCATC
TACAACATGGCCTCAGTCGACTTTGATGGCTTCTTTGCCGCCTTCCTCCCAGAGTTCCTGACCAGCTGTG
ATGGTGTGGATGCCAACCAGAAAAGTGTGCTGGGGCGGAATTTCAAGATGGATCGGGACCTGCCCTCATT
CACCCAGAATGTGCACAGGCTGGTCAACGACCTGCGCTACTACAGACTCTGCAACGACAGCCTGCCCCCT
GGCACTGTGAAGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC214839 protein sequence
Red=Cloning site Green=Tags(s)

MASEEASLRALESLMTEFFHDCTTNERKREIEELLNNFAQQIGAWRFCLYFLSSTRNDYVMMYSLTVFEN
LINKMWLGVPSQDKMEIRSCLPKLLLAHHKTLPYFIRNKLCKVIVDIGRQDWPMFYHDFFTNILQLIQSP
VTTPLGLIMLKTTSEELACPREDLSVARKEELRKLLLDQVQTVLGLLTGILETVWDKHSVTAATPPPSPT
SGESGDLLSNLLQSPSSAKLLNQPIPILDVESEYICSLALECLAHLFSWIPLSASITPSLLTTIFHFARF
GCDIRARKMASVNGSSQNCVSGQERGRLGVLAMSCINELMSKNCVPMEFEEYLLRMFQQTFYLLQKITKD
NNAHTVKSRLEELDESYIEKFTDFLRLFVSVHLRRIESYSQFPVVEFLTLLFKYTFHQPTHEGYFSCLDI
WTLFLDYLTSKIKSRLGDKEAVLNRYEDALVLLLTEVLNRIQFRYNQAQLEELDDETLDDDQQTEWQRYL
RQSLEVVAKVMELLPTHAFSTLFPVLQDNLEVYLGLQQFIVTSGSGHRLNITAENDCRRLHCSLRDLSSL
LQAVGRLAEYFIGDVFAARFNDALTVVERLVKVTLYGSQIKLYNIETAVPSVLKPDLIDVHAQSLAALQA
YSHWLAQYCSEVHRQNTQQFVTLISTTMDAITPLISTKVQDKLLLSACHLLVSLATTVRPVFLISIPAVQ
KVFNRITDASALRLVDKAQVLVCRALSNILLLPWPNLPENEQQWPVRSINHASLISALSRDYRNLKPSAV
APQRKMPLDDTKLIIHQTLSVLEDIVENISGESTKSRQICYQSLQESVQVSLALFPAFIHQSDVTDEMLS
FFLTLFRGLRVQMGVPFTEQIIQTFLNMFTREQLAESILHEGSTGCRVVEKFLKILQVVVQEPGQVFKPF
LPSIIALCMEQVYPIIAERPSPDVKAELFELLFRTLHHNWRYFFKSTVLASVQRGIAEEQMENEPQFSAI
MQAFGQSFLQPDIHLFKQNLFYLETLNTKQKLYHKKIFRTAMLFQFVNVLLQVLVHKSHDLLQEEIGIAI
YNMASVDFDGFFAAFLPEFLTSCDGVDANQKSVLGRNFKMDRDLPSFTQNVHRLVNDLRYYRLCNDSLPP
GTVKL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_015171
ORF Size 3375 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_015171.4
RefSeq Size 4504 bp
RefSeq ORF 3378 bp
Locus ID 23214
UniProt ID Q96QU8
Cytogenetics 16p12.1
MW 128.9 kDa
Summary The protein encoded by this gene is a member of the importin-beta family. Members of this family are regulated by the GTPase Ran to mediate transport of cargo across the nuclear envelope. This protein has been shown to mediate nuclear export of profilin-actin complexes. A pseudogene of this gene is located on the long arm of chromosome 14. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Aug 2012]
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.