QRICH1 (NM_017730) Human Tagged ORF Clone

SKU RC213114

QRICH1 (Myc-DDK-tagged)-Human glutamine-rich 1 (QRICH1), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: QRICH1
• Synonyms: AB-DIP; VERBRAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC213114 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAATAATTCCCTAGAGAACACCATCTCCTTTGAAGAGTACATCCGAGTAAAGGCACGGTCTGTCCCGC; AACACAGGATGAAGGAATTTCTGGACTCACTGGCCTCTAAGGGGCCAGAAGCCCTTCAGGAGTTCCAGCA; GACAGCCACCACTACCATGGTGTACCAACAGGGTGGGAACTGCATATACACAGACAGCACTGAAGTGGCT; GGGTCTTTGCTTGAACTTGCCTGTCCAGTCACCACCAGTGTTCAGCCACAAACCCAGCAAGAACAGCAGA; TCCAGGTTCAGCAGCCGCAGCAGGTTCAGGTCCAGGTGCAGGTACAGCAGTCTCCGCAACAGGTCTCGGC; TCAGCTCTCCCCACAACTCACCGTTCACCAGCCTACTGAGCAACCCATCCAGGTCCAGGTGCAGATCCAA; GGCCAGGCACCACAGTCAGCAGCCCCCTCCATTCAGACCCCGTCTCTGCAGAGTCCCAGTCCCTCGCAGC; TGCAAGCAGCTCAGATCCAGGTGCAGCACGTGCAAGCAGCCCAGCAGATCCAGGCTGCAGAAATCCCGGA; GGAGCACATCCCACATCAGCAAATCCAGGCTCAGCTGGTGGCTGGCCAGTCTCTTGCTGGTGGTCAGCAG; ATCCAAATCCAGACCGTGGGTGCCCTTTCCCCACCACCATCCCAGCAGGGCTCACCCCGGGAAGGGGAGC; GGCGGGTTGGCACGGCCAGTGTCCTCCAACCAGTGAAGAAGCGCAAAGTGGACATGCCCATCACTGTGTC; CTACGCCATCTCAGGGCAGCCGGTGGCCACCGTGCTGGCCATTCCACAGGGCCAGCAGCAGAGTTATGTG; TCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGGACCATTACTAGCC; CTACAGGAGAAACCTGGACCATCCCTGTTTATTCTGCCCAGCCCCGGGGGGACCCTCAGCAGCAGAGCAT; TACCCACATTGCCATTCCCCAGGAAGCCTACAACGCAGTTCACGTCAGTGGCTCACCCACAGCCCTGGCA; GCTGTTAAGCTGGAGGATGACAAGGAGAAGATGGTGGGCACCACATCTGTAGTGAAAAACTCCCATGAAG; AGGTAGTGCAGACCCTTGCAAACTCTCTCTTTCCAGCACAGTTCATGAATGGCAACATCCACATTCCAGT; GGCTGTGCAGGCTGTGGCAGGCACGTACCAGAATACGGCTCAAACTGTCCATATATGGGACCCCCAACAG; CAGCCGCAGCAGCAAACTCCCCAGGAACAGACACCACCACCACAGCAGCAGCAGCAGCAACTCCAAGTTA; CTTGTTCAGCTCAAACTGTCCAGGTTGCTGAAGTTGAACCACAGTCACAGCCACAGCCTTCCCCAGAACT; TCTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAAGTATGGAAAAACTGGGCCCAGACCAAGAAT; GCTGAACTAGAGAAGGATGCTCAGAACAGATTGGCACCCATTGGGAGGCGCCAACTGCTGCGATTCCAGG; AAGATCTCATCTCCTCTGCTGTGGCAGAGTTGAATTATGGGCTCTGTCTAATGACACGGGAAGCTCGAAA; TGGAGAAGGTGAACCCTATGACCCAGATGTGCTCTACTATATTTTCCTGTGTATTCAAAAGTATCTTTTT; GAAAATGGAAGGGTAGATGACATTTTCTCCGATCTTTATTATGTTCGGTTCACGGAGTGGCTACATGAAG; TTCTGAAGGATGTTCAGCCCCGGGTCACTCCACTTGGCTATGTCTTGCCCAGCCACGTGACTGAGGAGAT; GCTATGGGAGTGCAAGCAGCTTGGGGCTCACTCCCCCTCCACCTTGCTGACCACCCTCATGTTCTTTAAT; ACCAAGTACTTCCTATTGAAGACAGTGGACCAGCACATGAAGCTGGCCTTCTCCAAGGTCTTGCGACAGA; CAAAGAAGAACCCCTCTAATCCCAAGGATAAAAGCACGAGTATCCGGTACTTGAAGGCCCTTGGAATACA; CCAGACTGGCCAGAAAGTTACAGATGACATGTATGCAGAACAGACGGAAAATCCAGAGAATCCATTGAGA; TGTCCCATCAAGCTCTATGATTTCTACCTCTTCAAATGCCCCCAGAGTGTGAAAGGCCGGAATGACACCT; TTTACCTGACACCTGAGCCAGTGGTGGCCCCCAACAGCCCAATCTGGTACTCAGTCCAGCCTATCAGCAG; AGAGCAGATGGGACAAATGCTGACGCGGATCCTGGTGATAAGAGAAATTCAGGAGGCCATCGCAGTGGCC; AATGCAAGCACTATGCAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC213114 protein sequence; Red=Cloning site Green=Tags(s); ; MNNSLENTISFEEYIRVKARSVPQHRMKEFLDSLASKGPEALQEFQQTATTTMVYQQGGNCIYTDSTEVA; GSLLELACPVTTSVQPQTQQEQQIQVQQPQQVQVQVQVQQSPQQVSAQLSPQLTVHQPTEQPIQVQVQIQ; GQAPQSAAPSIQTPSLQSPSPSQLQAAQIQVQHVQAAQQIQAAEIPEEHIPHQQIQAQLVAGQSLAGGQQ; IQIQTVGALSPPPSQQGSPREGERRVGTASVLQPVKKRKVDMPITVSYAISGQPVATVLAIPQGQQQSYV; SLRPDLLTVDSAHLYSATGTITSPTGETWTIPVYSAQPRGDPQQQSITHIAIPQEAYNAVHVSGSPTALA; AVKLEDDKEKMVGTTSVVKNSHEEVVQTLANSLFPAQFMNGNIHIPVAVQAVAGTYQNTAQTVHIWDPQQ; QPQQQTPQEQTPPPQQQQQQLQVTCSAQTVQVAEVEPQSQPQPSPELLLPNSLKPEEGLEVWKNWAQTKN; AELEKDAQNRLAPIGRRQLLRFQEDLISSAVAELNYGLCLMTREARNGEGEPYDPDVLYYIFLCIQKYLF; ENGRVDDIFSDLYYVRFTEWLHEVLKDVQPRVTPLGYVLPSHVTEEMLWECKQLGAHSPSTLLTTLMFFN; TKYFLLKTVDQHMKLAFSKVLRQTKKNPSNPKDKSTSIRYLKALGIHQTGQKVTDDMYAEQTENPENPLR; CPIKLYDFYLFKCPQSVKGRNDTFYLTPEPVVAPNSPIWYSVQPISREQMGQMLTRILVIREIQEAIAVA; NASTMH; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017730
• ORF Size: 2328 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017730.3, NP_060200.2
• RefSeq Size: 3331 bp
• RefSeq ORF: 2331 bp
• Locus ID: 54870
• UniProt ID: Q2TAL8
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• MW: 86.4 kDa
• Summary: Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR) which is an ER-stress response pathway (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (PubMed:33384352). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (PubMed:33384352). Upregulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152).[UniProtKB/Swiss-Prot Function]