QRICH1 (NM_017730) Human Untagged Clone

SKU SC318260

QRICH1 (untagged)-Human glutamine-rich 1 (QRICH1), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: QRICH1
• Synonyms: AB-DIP; VERBRAS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC318260 representing NM_017730.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGAATAATTCCCTAGAGAACACCATCTCCTTTGAAGAGTACATCCGAGTAAAGGCACGGTCTGTCCCG; CAACACAGGATGAAGGAATTTCTGGACTCACTGGCCTCTAAGGGGCCAGAAGCCCTTCAGGAGTTCCAG; CAGACAGCCACCACTACCATGGTGTACCAACAGGGTGGGAACTGCATATACACAGACAGCACTGAAGTG; GCTGGGTCTTTGCTTGAACTTGCCTGTCCAGTCACCACCAGTGTTCAGCCACAAACCCAGCAAGAACAG; CAGATCCAGGTTCAGCAGCCGCAGCAGGTTCAGGTCCAGGTGCAGGTACAGCAGTCTCCGCAACAGGTC; TCGGCTCAGCTCTCCCCACAACTCACCGTTCACCAGCCTACTGAGCAACCCATCCAGGTCCAGGTGCAG; ATCCAAGGCCAGGCACCACAGTCAGCAGCCCCCTCCATTCAGACCCCGTCTCTGCAGAGTCCCAGTCCC; TCGCAGCTGCAAGCAGCTCAGATCCAGGTGCAGCACGTGCAAGCAGCCCAGCAGATCCAGGCTGCAGAA; ATCCCGGAGGAGCACATCCCACATCAGCAAATCCAGGCTCAGCTGGTGGCTGGCCAGTCTCTTGCTGGT; GGTCAGCAGATCCAAATCCAGACCGTGGGTGCCCTTTCCCCACCACCATCCCAGCAGGGCTCACCCCGG; GAAGGGGAGCGGCGGGTTGGCACGGCCAGTGTCCTCCAACCAGTGAAGAAGCGCAAAGTGGACATGCCC; ATCACTGTGTCCTACGCCATCTCAGGGCAGCCGGTGGCCACCGTGCTGGCCATTCCACAGGGCCAGCAG; CAGAGTTATGTGTCTTTGAGGCCAGACTTACTGACAGTAGACAGTGCCCACCTGTACAGTGCCACTGGG; ACCATTACTAGCCCTACAGGAGAAACCTGGACCATCCCTGTTTATTCTGCCCAGCCCCGGGGGGACCCT; CAGCAGCAGAGCATTACCCACATTGCCATTCCCCAGGAAGCCTACAACGCAGTTCACGTCAGTGGCTCA; CCCACAGCCCTGGCAGCTGTTAAGCTGGAGGATGACAAGGAGAAGATGGTGGGCACCACATCTGTAGTG; AAAAACTCCCATGAAGAGGTAGTGCAGACCCTTGCAAACTCTCTCTTTCCAGCACAGTTCATGAATGGC; AACATCCACATTCCAGTGGCTGTGCAGGCTGTGGCAGGCACGTACCAGAATACGGCTCAAACTGTCCAT; ATATGGGACCCCCAACAGCAGCCGCAGCAGCAAACTCCCCAGGAACAGACACCACCACCACAGCAGCAG; CAGCAGCAACTCCAAGTTACTTGTTCAGCTCAAACTGTCCAGGTTGCTGAAGTTGAACCACAGTCACAG; CCACAGCCTTCCCCAGAACTTCTGCTTCCAAATTCTTTGAAGCCAGAAGAAGGGCTTGAAGTATGGAAA; AACTGGGCCCAGACCAAGAATGCTGAACTAGAGAAGGATGCTCAGAACAGATTGGCACCCATTGGGAGG; CGCCAACTGCTGCGATTCCAGGAAGATCTCATCTCCTCTGCTGTGGCAGAGTTGAATTATGGGCTCTGT; CTAATGACACGGGAAGCTCGAAATGGAGAAGGTGAACCCTATGACCCAGATGTGCTCTACTATATTTTC; CTGTGTATTCAAAAGTATCTTTTTGAAAATGGAAGGGTAGATGACATTTTCTCCGATCTTTATTATGTT; CGGTTCACGGAGTGGCTACATGAAGTTCTGAAGGATGTTCAGCCCCGGGTCACTCCACTTGGCTATGTC; TTGCCCAGCCACGTGACTGAGGAGATGCTATGGGAGTGCAAGCAGCTTGGGGCTCACTCCCCCTCCACC; TTGCTGACCACCCTCATGTTCTTTAATACCAAGTACTTCCTATTGAAGACAGTGGACCAGCACATGAAG; CTGGCCTTCTCCAAGGTCTTGCGACAGACAAAGAAGAACCCCTCTAATCCCAAGGATAAAAGCACGAGT; ATCCGGTACTTGAAGGCCCTTGGAATACACCAGACTGGCCAGAAAGTTACAGATGACATGTATGCAGAA; CAGACGGAAAATCCAGAGAATCCATTGAGATGTCCCATCAAGCTCTATGATTTCTACCTCTTCAAATGC; CCCCAGAGTGTGAAAGGCCGGAATGACACCTTTTACCTGACACCTGAGCCAGTGGTGGCCCCCAACAGC; CCAATCTGGTACTCAGTCCAGCCTATCAGCAGAGAGCAGATGGGACAAATGCTGACGCGGATCCTGGTG; ATAAGAGAAATTCAGGAGGCCATCGCAGTGGCCAATGCAAGCACTATGCACTGA; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_017730
• Insert Size: 2331 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_017730.3
• RefSeq Size: 3633 bp
• RefSeq ORF: 2331 bp
• Locus ID: 54870
• UniProt ID: Q2TAL8
• Cytogenetics: 3p21.31
• Protein Families: Druggable Genome
• MW: 86.4 kDa
• Summary: Transcriptional regulator that acts as a mediator of the integrated stress response (ISR) through transcriptional control of protein homeostasis under conditions of ER stress (PubMed:33384352). Controls the outcome of the unfolded protein response (UPR) which is an ER-stress response pathway (PubMed:33384352). ER stress induces QRICH1 translation by a ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced QRICH1 regulates a transcriptional program associated with protein translation, protein secretion-mediated proteotoxicity and cell death during the terminal UPR (PubMed:33384352). May cooperate with ATF4 transcription factor signaling to regulate ER homeostasis which is critical for cell viability (PubMed:33384352). Upregulates CASP3/caspase-3 activity in epithelial cells under ER stress. Central regulator of proteotoxicity associated with ER stress-mediated inflammatory diseases in the intestines and liver (PubMed:33384352). Involved in chondrocyte hypertrophy, a process required for normal longitudinal bone growth (PubMed:30281152).[UniProtKB/Swiss-Prot Function]; Transcript Variant: This variant (1) represents the longest transcript. Variants 1-8 encode the same protein.