ADAMTS8 (NM_007037) Human Tagged ORF Clone

SKU RC212446

ADAMTS8 (Myc-DDK-tagged)-Human ADAM metallopeptidase with thrombospondin type 1 motif, 8 (ADAMTS8)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: ADAMTS8
• Synonyms: ADAM-TS8; METH2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC212446 representing NM_007037; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCTCCCCGCCCCCGCCGCCCCCCGGTGGCCTCCGCTCCTGCTGCTGCTGCTGCTGCTGCCGCTGGCCC; GCGGCGCCCCGGCCCGGCCCGCAGCCGGGGGGCAGGCCTCGGAGCTGGTGGTGCCCACGCGGTTGCCCGG; CAGCGCGGGCGAGCTCGCGCTCCACCTGTCCGCCTTCGGCAAGGGCTTCGTGCTGCGCCTGGCGCCCGAC; GACAGCTTCCTGGCGCCCGACTTCAAGATCGAGCGCCTCGGGGGCTCCGGCCGGGCGACCGGGGGCGAGC; GGGGGCTGCGCGGCTGCTTCTTCTCCGGCACCGTGAATGGGGAGCCCGAGTCGCTGGCGGCGGTCAGCCT; GTGCCGCGGGCTGAGCGGCTCCTTCCTGCTGGACGGCGAGGAGTTCACCATCCAGCCGCAGGGCGCGGGG; GGCTCCCTGGCTCAGCCGCACCGCCTGCAGCGCTGGGGTCCCGCCGGAGCCCGCCCCCTCCCGCGAGGAC; CCGAGTGGGAGGTGGAGACGGGAGAGGGTCAGAGGCAGGAGAGAGGAGACCACCAGGAGGACAGCGAGGA; GGAGAGCCAAGAAGAGGAGGCAGAAGGCGCTAGCGAGCCGCCACCGCCCCTGGGGGCCACGAGTAGGACC; AAGCGGTTTGTGTCTGAGGCGCGCTTCGTGGAGACGCTGCTGGTGGCCGATGCGTCCATGGCTGCCTTCT; ACGGGGCCGACCTGCAGAACCACATCCTGACGTTAATGTCTGTGGCAGCCCGAATCTACAAGCACCCCAG; CATCAAGAATTCCATCAACCTGATGGTGGTAAAAGTGCTGATCGTAGAAGATGAAAAATGGGGCCCAGAG; GTGTCCGACAATGGGGGGCTTACACTGCGTAACTTCTGCAACTGGCAGCGGCGTTTCAACCAGCCCAGCG; ACCGCCACCCAGAGCACTACGACACGGCCATCCTGCTCACCAGACAGAACTTCTGTGGGCAGGAGGGGCT; GTGTGACACCCTGGGTGTGGCAGACATCGGGACCATTTGTGACCCCAACAAAAGCTGCTCCGTGATCGAG; GATGAGGGGCTCCAGGCGGCCCACACCCTGGCCCATGAACTAGGGCACGTCCTCAGCATGCCCCACGACG; ACTCCAAGCCCTGCACACGGCTCTTCGGGCCCATGGGCAAGCACCACGTGATGGCACCGCTGTTCGTCCA; CCTGAACCAGACGCTGCCCTGGTCCCCCTGCAGCGCCATGTATCTCACAGAGCTTCTGGACGGCGGGCAC; GGAGACTGTCTCCTGGATGCCCCTGCTGCGGCCCTGCCCCTCCCCACAGGCCTCCCGGGCCGCATGGCCC; TGTACCAGCTGGACCAGCAGTGCAGGCAGATCTTTGGGCCGGATTTCCGCCACTGCCCCAACACCTCTGC; TCAGGACGTCTGCGCCCAGCTTTGGTGCCACACTGATGGGGCTGAGCCCCTGTGCCACACGAAGAATGGC; AGCCTGCCCTGGGCTGACGGCACGCCGTGCGGGCCTGGGCACCTCTGCTCAGAAGGCAGCTGTCTACCTG; AGGAGGAAGTGGAGAGGCCCAAGCCCGTGGTAGATGGAGGCTGGGCACCGTGGGGACCCTGGGGAGAATG; TTCTCGGACCTGTGGAGGAGGAGTACAGTTTTCACACCGTGAGTGCAAGGACCCCGAGCCTCAGAATGGA; GGAAGATACTGCCTGGGTCGGAGAGCCAAGTACCAGTCATGCCACACGGAGGAATGCCCCCCTGACGGGA; AAAGCTTCAGGGAGCAGCAGTGTGAGAAGTATAATGCCTACAATTACACTGACATGGACGGGAATCTCCT; GCAGTGGGTCCCCAAGTATGCTGGGGTGTCCCCCCGGGACCGCTGCAAGTTGTTCTGCCGAGCCCGGGGG; AGGAGCGAGTTCAAAGTGTTCGAGGCCAAGGTGATTGATGGCACCCTGTGTGGGCCAGAAACACTGGCCA; TCTGTGTCCGTGGCCAGTGTGTCAAGGCCGGCTGTGACCATGTGGTGGACTCGCCTCGGAAGCTGGACAA; ATGCGGGGTGTGTGGGGGCAAAGGCAACTCCTGCAGGAAGGTCTCCGGGTCCCTCACCCCCACCAATTAT; GGCTACAATGACATTGTCACCATCCCAGCTGGTGCCACTAATATTGACGTGAAGCAGCGGAGCCACCCGG; GTGTGCAGAACGATGGGAACTACCTGGCGCTGAAGACGGCTGATGGGCAGTACCTGCTCAACGGCAACCT; GGCCATCTCTGCCATAGAGCAGGACATCTTGGTGAAGGGGACCATCCTGAAGTACAGCGGCTCCATCGCC; ACCCTGGAGCGCCTGCAGAGCTTCCGGCCCTTGCCAGAGCCTCTGACAGTGCAGCTCCTGACAGTCCCTG; GCGAGGTCTTCCCCCCAAAAGTCAAATACACCTTCTTTGTTCCTAATGACGTGGACTTTAGCATGCAGAG; CAGCAAAGAGAGAGCAACCACCAACATCATCCAGCCGCTGCTCCACGCACAGTGGGTGCTGGGGGACTGG; TCTGAGTGCTCTAGCACCTGCGGGGCCGGCTGGCAGAGGCGAACTGTAGAGTGCAGGGACCCCTCCGGCC; AGGCCTCTGCCACCTGCAACAAGGCTCTGAAACCCGAGGATGCCAAGCCCTGCGAAAGCCAGCTGTGCCC; CCTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC212446 representing NM_007037; Red=Cloning site Green=Tags(s); ; MLPAPAAPRWPPLLLLLLLLPLARGAPARPAAGGQASELVVPTRLPGSAGELALHLSAFGKGFVLRLAPD; DSFLAPDFKIERLGGSGRATGGERGLRGCFFSGTVNGEPESLAAVSLCRGLSGSFLLDGEEFTIQPQGAG; GSLAQPHRLQRWGPAGARPLPRGPEWEVETGEGQRQERGDHQEDSEEESQEEEAEGASEPPPPLGATSRT; KRFVSEARFVETLLVADASMAAFYGADLQNHILTLMSVAARIYKHPSIKNSINLMVVKVLIVEDEKWGPE; VSDNGGLTLRNFCNWQRRFNQPSDRHPEHYDTAILLTRQNFCGQEGLCDTLGVADIGTICDPNKSCSVIE; DEGLQAAHTLAHELGHVLSMPHDDSKPCTRLFGPMGKHHVMAPLFVHLNQTLPWSPCSAMYLTELLDGGH; GDCLLDAPAAALPLPTGLPGRMALYQLDQQCRQIFGPDFRHCPNTSAQDVCAQLWCHTDGAEPLCHTKNG; SLPWADGTPCGPGHLCSEGSCLPEEEVERPKPVVDGGWAPWGPWGECSRTCGGGVQFSHRECKDPEPQNG; GRYCLGRRAKYQSCHTEECPPDGKSFREQQCEKYNAYNYTDMDGNLLQWVPKYAGVSPRDRCKLFCRARG; RSEFKVFEAKVIDGTLCGPETLAICVRGQCVKAGCDHVVDSPRKLDKCGVCGGKGNSCRKVSGSLTPTNY; GYNDIVTIPAGATNIDVKQRSHPGVQNDGNYLALKTADGQYLLNGNLAISAIEQDILVKGTILKYSGSIA; TLERLQSFRPLPEPLTVQLLTVPGEVFPPKVKYTFFVPNDVDFSMQSSKERATTNIIQPLLHAQWVLGDW; SECSSTCGAGWQRRTVECRDPSGQASATCNKALKPEDAKPCESQLCPL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007037
• ORF Size: 2664 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_007037.6
• RefSeq Size: 4028 bp
• RefSeq ORF: 2670 bp
• Locus ID: 11095
• UniProt ID: Q9UP79
• Cytogenetics: 11q24.3
• Protein Families: Druggable Genome, Protease, Secreted Protein
• MW: 96.3 kDa
• Summary: This gene encodes a member of the ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) protein family. Members of the family share several distinct protein modules, including a propeptide region, a metalloproteinase domain, a disintegrin-like domain, and a thrombospondin type 1 (TS) motif. Individual members of this family differ in the number of C-terminal TS motifs, and some have unique C-terminal domains. The encoded preproprotein is proteolytically processed to generate the mature enzyme. This enzyme contains two C-terminal TS motifs, and disrupts angiogenesis in vivo. A number of disorders have been mapped in the vicinity of this gene, most notably lung neoplasms. Reduced expression of this gene has been observed in multiple human cancers and this gene has been proposed as a potential tumor suppressor. [provided by RefSeq, Feb 2016]