Apolipoprotein A V (APOA5) (NM_052968) Human Tagged ORF Clone
SKU
RC211023
APOA5 (Myc-DDK-tagged)-Human apolipoprotein A-V (APOA5), transcript variant 1
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TrueORF Gold
Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Target Symbol | Apolipoprotein A V |
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Synonyms | APOAV; RAP3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC211023 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAGCATGGCTGCCGTGCTCACCTGGGCTCTGGCTCTTCTTTCAGCGTTTTCGGCCACCCAGGCAC GGAAAGGCTTCTGGGACTACTTCAGCCAGACCAGCGGGGACAAAGGCAGGGTGGAGCAGATCCATCAGCA GAAGATGGCTCGCGAGCCCGCGACCCTGAAAGACAGCCTTGAGCAAGACCTCAACAATATGAACAAGTTC CTGGAAAAGCTGAGGCCTCTGAGTGGGAGCGAGGCTCCTCGGCTCCCACAGGACCCGGTGGGCATGCGGC GGCAGCTGCAGGAGGAGTTGGAGGAGGTGAAGGCTCGCCTCCAGCCCTACATGGCAGAGGCGCACGAGCT GGTGGGCTGGAATTTGGAGGGCTTGCGGCAGCAACTGAAGCCCTACACGATGGATCTGATGGAGCAGGTG GCCCTGCGCGTGCAGGAGCTGCAGGAGCAGTTGCGCGTGGTGGGGGAAGACACCAAGGCCCAGTTGCTGG GGGGCGTGGACGAGGCTTGGGCTTTGCTGCAGGGACTGCAGAGCCGCGTGGTGCACCACACCGGCCGCTT CAAAGAGCTCTTCCACCCATACGCCGAGAGCCTGGTGAGCGGCATCGGGCGCCACGTGCAGGAGCTGCAC CGCAGTGTGGCTCCGCACGCCCCCGCCAGCCCCGCGCGCCTCAGTCGCTGCGTGCAGGTGCTCTCCCGGA AGCTCACGCTCAAGGCCAAGGCCCTGCACGCACGCATCCAGCAGAACCTGGACCAGCTGCGCGAAGAGCT CAGCAGAGCCTTTGCAGGCACTGGGACTGAGGAAGGGGCCGGCCCGGACCCCCAGATGCTCTCCGAGGAG GTGCGCCAGCGACTTCAGGCTTTCCGCCAGGACACCTACCTGCAGATAGCTGCCTTCACTCGCGCCATCG ACCAGGAGACTGAGGAGGTCCAGCAGCAGCTGGCGCCACCTCCACCAGGCCACAGTGCCTTCGCCCCAGA GTTTCAACAAACAGACAGTGGCAAGGTTCTGAGCAAGCTGCAGGCCCGTCTGGATGACCTGTGGGAAGAC ATCACTCACAGCCTTCATGACCAGGGCCACAGCCATCTGGGGGACCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC211023 protein sequence
Red=Cloning site Green=Tags(s) MASMAAVLTWALALLSAFSATQARKGFWDYFSQTSGDKGRVEQIHQQKMAREPATLKDSLEQDLNNMNKF LEKLRPLSGSEAPRLPQDPVGMRRQLQEELEEVKARLQPYMAEAHELVGWNLEGLRQQLKPYTMDLMEQV ALRVQELQEQLRVVGEDTKAQLLGGVDEAWALLQGLQSRVVHHTGRFKELFHPYAESLVSGIGRHVQELH RSVAPHAPASPARLSRCVQVLSRKLTLKAKALHARIQQNLDQLREELSRAFAGTGTEEGAGPDPQMLSEE VRQRLQAFRQDTYLQIAAFTRAIDQETEEVQQQLAPPPPGHSAFAPEFQQTDSGKVLSKLQARLDDLWED ITHSLHDQGHSHLGDP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_052968 |
ORF Size | 1098 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_052968.5 |
RefSeq Size | 1954 bp |
RefSeq ORF | 1101 bp |
Locus ID | 116519 |
UniProt ID | Q6Q788 |
Cytogenetics | 11q23.3 |
Domains | Apolipoprotein |
Protein Families | Druggable Genome, Secreted Protein |
Protein Pathways | PPAR signaling pathway |
MW | 41.2 kDa |
Summary | The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC211023L1 | Lenti ORF clone of Human apolipoprotein A-V (APOA5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
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RC211023L2 | Lenti ORF clone of Human apolipoprotein A-V (APOA5), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
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RC211023L3 | Lenti ORF clone of Human apolipoprotein A-V (APOA5), transcript variant 1, Myc-DDK-tagged | 10 ug |
$757.00
|
|
RC211023L4 | Lenti ORF clone of Human apolipoprotein A-V (APOA5), transcript variant 1, mGFP tagged | 10 ug |
$757.00
|
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RG211023 | APOA5 (tGFP-tagged) - Human apolipoprotein A-V (APOA5), transcript variant 1 | 10 ug |
$489.00
MSRP
$657.00
MSRP
$657.00
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SC120180 | APOA5 (untagged)-Human apolipoprotein A-V (APOA5), transcript variant 1 | 10 ug |
$457.00
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.