Apolipoprotein A V (APOA5) (NM_052968) Human Untagged Clone

SKU SC120180

APOA5 (untagged)-Human apolipoprotein A-V (APOA5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: Apolipoprotein A V
• Synonyms: APOAV; RAP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120180 sequence for NM_052968 edited (data generated by NextGen Sequencing); ATGGCAAGCATGGCTGCCGTGCTCACCTGGGCTCTGGCTCTTCTTTCAGCGTTTTCGGCC; ACCCAGGCACGGAAAGGCTTCTGGGACTACTTCAGCCAGACCAGCGGGGACAAAGGCAGG; GTGGAGCAGATCCATCAGCAGAAGATGGCTCGCGAGCCCGCGACCCTGAAAGACAGCCTT; GAGCAAGACCTCAACAATATGAACAAGTTCCTGGAAAAGCTGAGGCCTCTGAGTGGGAGC; GAGGCTCCTCGGCTCCCACAGGACCCGGTGGGCATGCGGCGGCAGCTGCAGGAGGAGTTG; GAGGAGGTGAAGGCTCGCCTCCAGCCCTACATGGCAGAGGCGCACGAGCTGGTGGGCTGG; AATTTGGAGGGCTTGCGGCAGCAACTGAAGCCCTACACGATGGATCTGATGGAGCAGGTG; GCCCTGCGCGTGCAGGAGCTGCAGGAGCAGTTGCGCGTGGTGGGGGAAGACACCAAGGCC; CAGTTGCTGGGGGGCGTGGACGAGGCTTGGGCTTTGCTGCAGGGACTGCAGAGCCGCGTG; GTGCACCACACCGGCCGCTTCAAAGAGCTCTTCCACCCATACGCCGAGAGCCTGGTGAGC; GGCATCGGGCGCCACGTGCAGGAGCTGCACCGCAGTGTGGCTCCGCACGCCCCCGCCAGC; CCCGCGCGCCTCAGTCGCTGCGTGCAGGTGCTCTCCCGGAAGCTCACGCTCAAGGCCAAG; GCCCTGCACGCACGCATCCAGCAGAACCTGGACCAGCTGCGCGAAGAGCTCAGCAGAGCC; TTTGCAGGCACTGGGACTGAGGAAGGGGCCGGCCCGGACCCCCAGATGCTCTCCGAGGAG; GTGCGCCAGCGACTTCAGGCTTTCCGCCAGGACACCTACCTGCAGATAGCTGCCTTCACT; CGCGCCATCGACCAGGAGACTGAGGAGGTCCAGCAGCAGCTGGCGCCACCTCCACCAGGC; CACAGTGCCTTCGCCCCAGAGTTTCAACAAACAGACAGTGGCAAGGTTCTGAGCAAGCTG; CAGGCCCGTCTGGATGACCTGTGGGAAGACATCACTCACAGCCTTCATGACCAGGGCCAC; AGCCATCTGGGGGACCCCTGA; ; Clone variation with respect to NM_052968.4;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_052968 unedited; GTTCAAATATTTGTAATACGAACTCACTATAGGGCGGCCGCGAATCGGCACCAGCCTCCC; TCCACCTGTCTTCTCAGAGCAGATAATGGCAAGCATGGCTGCCGTGCTCACCTGGGCTCT; GGCTCTTCTTTCAGCGTTTTCGGCCACCCAGGCACGGAAAGGCTTCTGGGACTACTTCAG; CCAGACCAGCGGGGACAAAGGCAGGGTGGAGCAGATCCATCAGCAGAAGATGGCTCGCGA; GCCCGCGACCCTGAAAGACAGCCTTGAGCAAGACCTCAACAATATGAACAAGTTCCTGGA; AAAGCTGAGGCCTCTGAGTGGGAGCGAGGCTCCTCGGCTCCCACAGGACCCGGTGGGCAT; GCGGCGGCAGCTGCAGGAGGAGTTGGAGGAGGTGAAGGCTCGCCTCCAGCCCTACATGGC; AGAGGCGCACGAGCTGGTGGGCTGGAATTTGGAGGGCTTGCGGCAGCAACTGAAGCCCTA; CACGATGGATCTGATGGAGCAGGTGGCCCTGCGCGTGCAGGAGCTGCAGGAGCAGTTGCG; CGTGGTGGGGGAAGACACCAAGGCCCAGTTGCTGGGGGGCGTGGACGAGGCTTGGGCTTT; GCTGCAGGGACTGCAGAGCCGCGTGGTGCACCACACCGGCCGCTTCAAAGAGCTCTTCCA; CCCATACGCCGAGAGCCTGGTGAGCGGCATCGGGCGCCACGTGCANGAGCTGCACCGCAG; TGTGGCTCCGCACGCCCCCGCCAGCCCCGCGCGCCTCAGTCGCTGCGTGCAGGTGCTCTC; CCGGNAGCTCACGCTCAAGGCCAAGGGCCTGCACGCACGCATCCAGCAGAACCTGGACCA; GCTGCGCGAAGAGCTCAGCAGAACCCTTGCAGGCACTGGGACTGAGGAAAGGGCCGGCCC; GGACCCCAAATGCTCTCGAGGAGTGCGCCACGACTCCGGCTTTTCGCCCGGAACCTACCG; CGGAA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_052968 unedited; TTCNNCNAATTACTTGNACCGCGCCGCATTCNANGATCGAGTTTTTTTTTTTTTTTTTGC; AAAGCCTGGTGAATGTAATGCATCCAGATTGGGGAGTCGCAGGAGGCTGGATATGCAGGA; GACAGCAGCCCCTTTGGTGGCCTCCCTGTCCTGCACAGGACCTTCCACCCTCCACCCAAC; AGGCCACTTTCAAGGACTGAACCATGCTAGAGGCTCAGAGCCAAGGCTCCCCAGACAAGG; AGCTGGGAATGGGCCTGGGCAGGTAGATCCTCAGGGGTCCCCCAGATGGCTGTGGCCCTG; GTCATGAAGGCTGTGAGTGATGTCTTCCCACAGGTCATCCAGACGGGCCTGCAGCTTGCT; CAGAACCTTGCCACTGTCTGTTTGTTGAAACTCTGGGGCGAAGGCACTGTGGCCTGGTGG; AGGTGGCGCCAGCTGCTGCTGGACCTCCTCAGTCTCCTGGTCGATGGCGCGAGTGAAGGC; AGCTATCTGCAGGTAGGTGTCCTGGCGGAAAGCCTGAAGTCGCTGGCGCACCTCCTCGGA; GAGCATCTGGGGGTCCGGGCCGGCCCCTTCCTCAGTCCCAGTGCCTGCAAAGGCTCTGCT; GAGCTCTTCGCGCAGCTGGTCCAGGTTCTGCTGGATGCGTGCGTGCAGGGCCTTGGCCTT; GAGCGTGAGCTTCCGGGAGAGCACCTGCACGCAGCGACTNGAGCGCGCGGNGCTGGCGGG; GGCGTGCGGAGCCACACTGCGGTGCAGCTCCTGCACGTGGCGCCCCATGCCGCTCACCAG; GCTCTCGGCGTATGGGTGGGAGAGCTCTTTGAAGCCGGCCGGGTGTGGTGCACCACGCGG; CTCTTGCAC;
• Restriction Sites: NotI-NotI
• ACCN: NM_052968
• Insert Size: 1550 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_052968.3, NP_443200.2
• RefSeq Size: 1889 bp
• RefSeq ORF: 1101 bp
• Locus ID: 116519
• UniProt ID: Q6Q788
• Cytogenetics: 11q23.3
• Domains: Apolipoprotein
• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: PPAR signaling pathway
• Summary: The protein encoded by this gene is an apolipoprotein that plays an important role in regulating the plasma triglyceride levels, a major risk factor for coronary artery disease. It is a component of high density lipoprotein and is highly similar to a rat protein that is upregulated in response to liver injury. Mutations in this gene have been associated with hypertriglyceridemia and hyperlipoproteinemia type 5. This gene is located proximal to the apolipoprotein gene cluster on chromosome 11q23. Alternatively spliced transcript variants encoding the same protein have been identified. [provided by RefSeq, Oct 2009]; Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein.