Caspr2 (CNTNAP2) (NM_014141) Human Tagged ORF Clone

CAT#: RC210836

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CNTNAP2 (Myc-DDK-tagged)-Human contactin associated protein-like 2 (CNTNAP2)



  "NM_014141" in other vectors (6)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 1,737.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol CNTNAP2
Synonyms AUTS15; CASPR2; CDFE; NRXN4; PTHSL1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC210836 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGGCGGCTCCGCGCGCCGGCTGCGGGGCAGCGCTCCTGCTGTGGATTGTCAGCAGCTGCCTCTGCA
GAGCCTGGACGGCTCCCTCCACGTCCCAAAAATGTGATGAGCCACTTGTCTCTGGACTCCCCCATGTGGC
TTTCAGCAGCTCCTCCTCCATCTCTGGTAGCTATTCTCCCGGCTATGCCAAGATAAACAAGAGAGGAGGT
GCTGGGGGATGGTCTCCATCAGACAGCGACCATTATCAATGGCTTCAGGTTGACTTTGGCAATCGGAAGC
AGATCAGTGCCATTGCAACCCAAGGAAGGTATAGCAGCTCAGATTGGGTGACCCAATACCGGATGCTCTA
CAGCGACACAGGGAGAAACTGGAAACCCTATCATCAAGATGGGAATATCTGGGCATTTCCCGGAAACATT
AACTCTGACGGTGTGGTCCGGCACGAATTACAGCATCCGATTATTGCCCGCTATGTGCGCATAGTGCCTC
TGGATTGGAATGGAGAAGGTCGCATTGGACTCAGAATTGAAGTTTATGGCTGTTCTTACTGGGCTGATGT
TATCAACTTTGATGGCCATGTTGTATTACCATATAGATTCAGAAACAAGAAGATGAAAACACTGAAAGAT
GTCATTGCCTTGAACTTTAAGACGTCTGAAAGTGAAGGAGTAATCCTGCACGGAGAAGGACAGCAAGGAG
ATTACATTACCTTGGAACTGAAAAAAGCCAAGCTGGTCCTCAGTTTAAACTTAGGAAGCAACCAGCTTGG
CCCCATATATGGCCACACATCAGTGATGACAGGAAGTTTGCTGGATGACCACCACTGGCACTCTGTGGTC
ATTGAGCGCCAGGGGCGGAGCATTAACCTCACTCTGGACAGGAGCATGCAGCACTTCCGTACCAATGGAG
AGTTTGACTACCTGGACTTGGACTATGAGATAACCTTTGGAGGCATCCCTTTCTCTGGCAAGCCCAGCTC
CAGCAGTAGAAAGAATTTCAAAGGCTGCATGGAAAGCATCAACTACAATGGCGTCAACATTACTGATCTT
GCCAGAAGGAAGAAATTAGAGCCCTCAAATGTGGGAAATTTGAGCTTTTCTTGTGTGGAACCCTATACGG
TGCCTGTCTTTTTCAACGCTACAAGTTACCTGGAGGTGCCCGGACGGCTTAACCAGGACCTGTTCTCAGT
CAGTTTCCAGTTTAGGACATGGAACCCCAATGGTCTCCTGGTCTTCAGTCACTTTGCGGATAATTTGGGC
AATGTGGAGATTGACCTCACTGAAAGCAAAGTGGGTGTTCACATCAACATCACACAGACCAAGATGAGCC
AAATCGATATTTCCTCAGGTTCTGGGTTGAATGATGGACAGTGGCACGAGGTTCGCTTCCTAGCCAAGGA
AAATTTTGCTATTCTCACCATCGATGGAGATGAAGCATCAGCAGTTCGAACTAATAGTCCCCTTCAAGTT
AAAACTGGCGAGAAGTACTTTTTTGGAGGTTTTCTGAACCAGATGAATAACTCAAGTCACTCTGTCCTTC
AGCCTTCATTCCAAGGATGCATGCAGCTCATTCAAGTGGACGATCAACTTGTAAATTTATACGAAGTGGC
ACAAAGGAAGCCGGGAAGTTTCGCGAATGTCAGCATTGACATGTGTGCAATCATAGACAGATGTGTGCCC
AATCACTGTGAGCATGGTGGAAAGTGCTCGCAAACATGGGACAGCTTCAAATGCACTTGTGATGAGACAG
GATACAGTGGGGCCACCTGCCACAACTCTATCTACGAGCCTTCCTGTGAAGCCTACAAACACCTAGGACA
GACATCAAATTATTACTGGATAGATCCTGATGGCAGCGGACCTCTGGGGCCTCTGAAAGTTTACTGCAAC
ATGACAGAGGACAAAGTGTGGACCATAGTGTCTCATGACTTGCAGATGCAGACGCCTGTGGTCGGCTACA
ACCCAGAAAAATACTCAGTGACACAGCTCGTTTACAGCGCCTCCATGGACCAGATAAGTGCCATCACTGA
CAGTGCCGAGTACTGCGAGCAGTATGTCTCCTATTTCTGCAAGATGTCAAGATTGTTGAACACCCCAGAT
GGAAGCCCTTACACTTGGTGGGTTGGCAAAGCCAACGAGAAGCACTACTACTGGGGAGGCTCTGGGCCTG
GAATCCAGAAATGTGCCTGCGGCATCGAACGCAACTGCACAGATCCCAAGTACTACTGTAACTGCGACGC
GGACTACAAGCAATGGAGGAAGGATGCTGGTTTCTTATCATACAAAGATCACCTGCCAGTGAGCCAAGTG
GTGGTTGGAGATACTGACCGTCAAGGCTCAGAAGCCAAATTGAGCGTAGGTCCTCTGCGCTGCCAAGGAG
ACAGGAATTATTGGAATGCCGCCTCTTTCCCAAACCCATCCTCCTACCTGCACTTCTCTACTTTCCAAGG
GGAAACTAGCGCTGACATTTCTTTCTACTTCAAAACATTAACCCCCTGGGGAGTGTTTCTTGAAAATATG
GGAAAGGAAGATTTCATCAAGCTGGAGCTGAAGTCTGCCACAGAAGTGTCCTTTTCATTTGATGTGGGAA
ATGGGCCAGTAGAGATTGTAGTGAGGTCACCAACCCCTCTCAACGATGACCAGTGGCACCGGGTCACTGC
AGAGAGGAATGTCAAGCAGGCCAGCCTACAGGTGGACCGGCTACCGCAGCAGATCCGCAAGGCCCCAACA
GAAGGCCACACCCGCCTGGAGCTCTACAGCCAGTTATTTGTGGGTGGTGCTGGGGGCCAGCAGGGCTTCC
TGGGTTGCATCCGCTCCTTGAGGATGAATGGGGTGACACTTGACCTGGAGGAAAGAGCAAAGGTCACATC
TGGGTTCATATCCGGATGCTCGGGCCATTGCACCAGCTATGGAACAAACTGTGAAAATGGAGGCAAATGC
CTAGAGAGATACCACGGTTACTCCTGCGATTGCTCTAATACTGCATATGATGGAACATTTTGCAACAAAG
ATGTTGGTGCATTTTTTGAAGAAGGGATGTGGCTACGATATAACTTTCAGGCACCAGCAACAAATGCCAG
AGACTCCAGCAGCAGAGTAGACAACGCTCCCGACCAGCAGAACTCCCACCCGGACCTGGCACAGGAGGAG
ATCCGCTTCAGCTTCAGCACCACCAAGGCGCCCTGCATTCTCCTCTACATCAGCTCCTTCACCACAGACT
TCTTGGCAGTCCTCGTCAAACCCACTGGAAGCTTACAGATTCGATACAACCTGGGTGGCACCCGAGAGCC
ATACAATATTGACGTAGACCACAGGAACATGGCCAATGGACAGCCCCACAGTGTCAACATCACCCGCCAC
GAGAAGACCATCTTTCTCAAGCTCGATCATTATCCTTCTGTGAGTTACCATCTGCCAAGTTCATCCGACA
CCCTCTTCAATTCTCCCAAGTCGCTCTTTCTGGGAAAAGTTATAGAAACAGGGAAAATTGACCAAGAGAT
TCACAAATACAACACCCCAGGATTCACTGGTTGCCTCTCCAGAGTCCAGTTCAACCAGATCGCCCCTCTC
AAGGCCGCCTTGAGGCAGACAAACGCCTCGGCTCACGTCCACATCCAGGGCGAGCTGGTGGAGTCCAACT
GCGGGGCCTCGCCGCTGACCCTCTCCCCCATGTCGTCCGCCACCGACCCCTGGCACCTGGATCACCTGGA
TTCAGCCAGTGCGGATTTTCCATATAATCCAGGACAAGGCCAAGCTATAAGAAATGGAGTCAACAGAAAC
TCGGCTATCATTGGAGGCGTCATTGCTGTGGTGATTTTCACCATCCTGTGCACCCTGGTCTTCCTGATCC
GGTACATGTTCCGCCACAAGGGCACCTACCATACCAACGAAGCAAAGGGGGCGGAGTCGGCAGAGAGCGC
GGACGCCGCCATCATGAACAACGACCCCAACTTCACAGAGACCATTGATGAAAGCAAAAAGGAATGGCTC
ATT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC210836 protein sequence
Red=Cloning site Green=Tags(s)

MQAAPRAGCGAALLLWIVSSCLCRAWTAPSTSQKCDEPLVSGLPHVAFSSSSSISGSYSPGYAKINKRGG
AGGWSPSDSDHYQWLQVDFGNRKQISAIATQGRYSSSDWVTQYRMLYSDTGRNWKPYHQDGNIWAFPGNI
NSDGVVRHELQHPIIARYVRIVPLDWNGEGRIGLRIEVYGCSYWADVINFDGHVVLPYRFRNKKMKTLKD
VIALNFKTSESEGVILHGEGQQGDYITLELKKAKLVLSLNLGSNQLGPIYGHTSVMTGSLLDDHHWHSVV
IERQGRSINLTLDRSMQHFRTNGEFDYLDLDYEITFGGIPFSGKPSSSSRKNFKGCMESINYNGVNITDL
ARRKKLEPSNVGNLSFSCVEPYTVPVFFNATSYLEVPGRLNQDLFSVSFQFRTWNPNGLLVFSHFADNLG
NVEIDLTESKVGVHINITQTKMSQIDISSGSGLNDGQWHEVRFLAKENFAILTIDGDEASAVRTNSPLQV
KTGEKYFFGGFLNQMNNSSHSVLQPSFQGCMQLIQVDDQLVNLYEVAQRKPGSFANVSIDMCAIIDRCVP
NHCEHGGKCSQTWDSFKCTCDETGYSGATCHNSIYEPSCEAYKHLGQTSNYYWIDPDGSGPLGPLKVYCN
MTEDKVWTIVSHDLQMQTPVVGYNPEKYSVTQLVYSASMDQISAITDSAEYCEQYVSYFCKMSRLLNTPD
GSPYTWWVGKANEKHYYWGGSGPGIQKCACGIERNCTDPKYYCNCDADYKQWRKDAGFLSYKDHLPVSQV
VVGDTDRQGSEAKLSVGPLRCQGDRNYWNAASFPNPSSYLHFSTFQGETSADISFYFKTLTPWGVFLENM
GKEDFIKLELKSATEVSFSFDVGNGPVEIVVRSPTPLNDDQWHRVTAERNVKQASLQVDRLPQQIRKAPT
EGHTRLELYSQLFVGGAGGQQGFLGCIRSLRMNGVTLDLEERAKVTSGFISGCSGHCTSYGTNCENGGKC
LERYHGYSCDCSNTAYDGTFCNKDVGAFFEEGMWLRYNFQAPATNARDSSSRVDNAPDQQNSHPDLAQEE
IRFSFSTTKAPCILLYISSFTTDFLAVLVKPTGSLQIRYNLGGTREPYNIDVDHRNMANGQPHSVNITRH
EKTIFLKLDHYPSVSYHLPSSSDTLFNSPKSLFLGKVIETGKIDQEIHKYNTPGFTGCLSRVQFNQIAPL
KAALRQTNASAHVHIQGELVESNCGASPLTLSPMSSATDPWHLDHLDSASADFPYNPGQGQAIRNGVNRN
SAIIGGVIAVVIFTILCTLVFLIRYMFRHKGTYHTNEAKGAESAESADAAIMNNDPNFTETIDESKKEWL
I

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014141
ORF Size 3993 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014141.6
RefSeq Size 9894 bp
RefSeq ORF 3996 bp
Locus ID 26047
UniProt ID Q9UHC6
Cytogenetics 7q35-q36.1
Domains F5_F8_type_C, LamG, EGF
Protein Families Druggable Genome, Transmembrane
Protein Pathways Cell adhesion molecules (CAMs)
MW 148.2 kDa
Gene Summary This gene encodes a member of the neurexin family which functions in the vertebrate nervous system as cell adhesion molecules and receptors. This protein, like other neurexin proteins, contains epidermal growth factor repeats and laminin G domains. In addition, it includes an F5/8 type C domain, discoidin/neuropilin- and fibrinogen-like domains, thrombospondin N-terminal-like domains and a putative PDZ binding site. This protein is localized at the juxtaparanodes of myelinated axons, and mediates interactions between neurons and glia during nervous system development and is also involved in localization of potassium channels within differentiating axons. This gene encompasses almost 1.5% of chromosome 7 and is one of the largest genes in the human genome. It is directly bound and regulated by forkhead box protein P2, a transcription factor related to speech and language development. This gene has been implicated in multiple neurodevelopmental disorders, including Gilles de la Tourette syndrome, schizophrenia, epilepsy, autism, ADHD and intellectual disability. [provided by RefSeq, Jul 2017]

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