alpha Synuclein (SNCA) (NM_000345) Human Tagged ORF Clone
- TrueORF®
SKU
RC210606
SNCA (Myc-DDK-tagged)-Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1
$150.00
5 Days*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | alpha Synuclein |
| Synonyms | NACP; PARK1; PARK4; PD1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_000345 |
| ORF Size | 420 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_000345.4 |
| RefSeq Size | 1543 bp |
| RefSeq ORF | 423 bp |
| Locus ID | 6622 |
| UniProt ID | P37840 |
| Cytogenetics | 4q22.1 |
| Domains | Synuclein |
| Protein Families | Druggable Genome |
| Protein Pathways | Alzheimer's disease, Parkinson's disease |
| MW | 14.3 kDa |
| Summary | Alpha-synuclein is a member of the synuclein family, which also includes beta- and gamma-synuclein. Synucleins are abundantly expressed in the brain and alpha- and beta-synuclein inhibit phospholipase D2 selectively. SNCA may serve to integrate presynaptic signaling and membrane trafficking. Defects in SNCA have been implicated in the pathogenesis of Parkinson disease. SNCA peptides are a major component of amyloid plaques in the brains of patients with Alzheimer's disease. Alternatively spliced transcripts encoding different isoforms have been identified for this gene. provided by RefSeq, Feb 2016 |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_000345" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC210606L1 | Lenti ORF clone of Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210606L2 | Lenti ORF clone of Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1, mGFP tagged | 10 ug |
$450.00
|
|
| RC210606L3 | Lenti ORF clone of Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1, Myc-DDK-tagged | 10 ug |
$450.00
|
|
| RC210606L4 | Lenti ORF clone of Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1, mGFP tagged | 10 ug |
$450.00
|
|
| RG210606 | SNCA (tGFP-tagged) - Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1 | 10 ug |
$489.00
|
|
| SC119919 | SNCA (untagged)-Human synuclein, alpha (non A4 component of amyloid precursor) (SNCA), transcript variant 1 | 10 ug |
$150.00
|
|
Citations
Amyloid β,α-Synuclein and Amyloid β-α-Synuclein Combination Exert Significant but Different Alterations in Inflammatory Response Profile in Differentiated Human SH-SY5Y Cells
Keskin, Gezen-Ak, Dursun
ACS Omega (2023) 8 (48), 45519-45534 DOI: 10.1021/acsomega.3c05585
Keskin, Gezen-Ak, Dursun
ACS Omega (2023) 8 (48), 45519-45534 DOI: 10.1021/acsomega.3c05585
Cited by 7
AI Summary: OriGene products contributed to this study by providing the mock plasmid (pMVC6-Entry, cat no: PS100001) used as a negative control and the SNCA overexpression plasmid (Human Tagged ORF Clone TrueORFGold, cat no: RC210606) used to model α-synuclein pathology in differentiated SH-SY5Y cells.
Snippet: … (2) Negative control or MOCK plasmid treated group, which was treated with mock plasmid containing CMV promoter but no open reading frame (ORF) for any gene (pMVC6-Entry, mammalian vector, oriGene cat no: PS100001);this group was used to distinguish between mimicry and inhibitory/activatory effects and true effect caused by α-synuclein transfection 3. SNCA overexpression group consist;Negative control or MOCK plasmid treated group, which was treated with mock plasmid containing CMV promoter but no open reading frame (ORF) for any gene (pMVC6-Entry, mammalian vector, oriGene cat no: PS100001);this group was used to distinguish between mimicry and inhibitory/activatory effects and true effect caused by α-synuclein transfection 3. SNCA overexpression group consisted of the cells that were transfected with SNCA gene (human synuclein, α (non A4 component of amyloid precursor), transcript variant 1;SNCA NM_000345 Human Tagged ORF Clone TrueORFGold oriGene cat no: RC210606). …
α-Synuclein antisense transcript SNCA-AS1 regulates synapses- and aging-related genes suggesting its implication in Parkinson's disease
Rey, Pandini, Messa et al
Aging Cell (2021) 20 (12), e13504 DOI: 10.1111/acel.13504
Rey, Pandini, Messa et al
Aging Cell (2021) 20 (12), e13504 DOI: 10.1111/acel.13504
Cited by 31
AI Summary: OriGene products, specifically the pCMV6-AC-RFP construct for SNCA-AS1 and the alpha Synuclein SNCA NM_000345 Human Tagged ORF Clone (RG210606), were used to generate stable cell lines overexpressing SNCA-AS1 and SNCA, enabling the study of their roles in synaptic regulation and aging-related pathways in Parkinson’s disease.
Snippet: and 1% penicillin/streptomycin (Gibco) at 37°C in a 5% CO2 atmosphere. For SNCA‐AS1 stable transfection, a pCMV6‐AC‐RFP construct containing gentamicin‐disulfate G418 resistance was used (OriGene). For SNCA stable transfection, a custom pCMV6‐AC‐GFP construct containing G418 resistance was used (alpha Synuclein SNCA NM_000345 Human Tagged ORF Clone, RG210606, OriGene). The plasmids
Overexpression of α-Synuclein Reorganises Growth Factor Profile of Human Astrocytes
Şengül, Dursun, Verkhratsky et al
Mol. Neurobiol. (2020)
Şengül, Dursun, Verkhratsky et al
Mol. Neurobiol. (2020)
Cited by 23
AI Summary: OriGene products contributed to this study by providing the SNCA NM_000345 Human Tagged ORF Clone (cat no: RC210606) for α-synuclein overexpression and the pMVC6-Entry mock plasmid (cat no: PS100001) as a negative control, enabling the researchers to investigate how increased α-synuclein alters growth factor profiles in human astrocytes.
Snippet: Following experimental groups were established: (i) Control group;(ii) SNCA group of astrocytes transfected with SNCA gene (Human synuclein, α (non A4 component of amyloid precursor), transcript variant 1;SNCA NM_000345 Human Tagged ORF Clone TrueORFGold oriGene cat no: RC210606);(iii) Negative control or MOCK group, which was treated with mock plasmid containing CMV promoter and Myc-DDK tag but no open reading frame (ORF) for any gene (pMVC6-Entry, mammalian vector with C-terminal Myc-DDK Tag, oriGene cat no: PS100001). …
Inactive USP14 and inactive UCHL5 cause accumulation of distinct ubiquitinated proteins in mammalian cells
Chadchankar, Korboukh, Conway et al
PLoS ONE (2019) 14 (11), e0225145 DOI: 10.1371/journal.pone.0225145
Chadchankar, Korboukh, Conway et al
PLoS ONE (2019) 14 (11), e0225145 DOI: 10.1371/journal.pone.0225145
AI Summary: OriGene contributed plasmids for tau (4R/2N), α-synuclein, USP14, and UCHL5, which were used to express these proteins in mammalian cells for investigating the distinct effects of inactive USP14 and UCHL5 on the accumulation of ubiquitinated proteins.
Snippet: … Plasmids for tau (4R/2N), α-synuclein, USP14 and UCHL5 were purchased from Origene. Mutant plasmids USP14 C114A, USP14 WT ΔUBL, USP14 C114A ΔUBL and UCHL5 C88A were generated from the wildtype plasmid by Genscript. …
A stress-enhanced model for discovery of disease-modifying gene: Ece1-suppresses the toxicity of α-synuclein A30P
Chen, Tully
Neurobiol. Dis. (2018) 114, 153-163 DOI: 10.1016/j.nbd.2018.03.003
Chen, Tully
Neurobiol. Dis. (2018) 114, 153-163 DOI: 10.1016/j.nbd.2018.03.003
AI Summary: OriGene's human NACP140 cDNA (NM_000345.2) was used as the template to generate the A30P mutant α-synuclein construct for functional studies in this paper.
Snippet: Nuclear-localizing sequence (NLS) on RFP was generated by direct synthesis. A30P was created by creating a point mutation at the DNA codon of the 30th alanine, (G → C)CA, using human NACP140 (NM_000345.2;OriGene, MD, USA)
Unconventional secretion of misfolded proteins promotes adaptation to proteasome dysfunction in mammalian cells
Lee, Takahama, Zhang et al
Nat. Cell Biol. (2016) 18 (7), 765-76 DOI: 10.1038/ncb3372
Lee, Takahama, Zhang et al
Nat. Cell Biol. (2016) 18 (7), 765-76 DOI: 10.1038/ncb3372
Cited by 221
AI Summary: OriGene contributed to this study by providing the pCMV6-Entry vector (cat #PS100001), which was used for cloning the full-length cDNA of human CTMP for experimental analyses.
Snippet: The full-length cDNA of human CTMP was amplified using PCR from cDNA libraries and inserted into the pCMV6-Entry (cat #PS100001) vector purchased from OriGene (Rockville, MD, USA)
Showing 1-6 of 6 papers.
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