Protein kinase Y linked (PRKY) (NM_002760) Human Tagged ORF Clone
SKU
RC210438
PRKY (Myc-DDK-tagged)-Human protein kinase, Y-linked (PRKY)
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Protein expression verified by Western blot, fully sequenced and in stock
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Product Data | |
Type | Human Tagged ORF Clone |
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Target Symbol | Protein kinase Y linked |
Synonyms | OTTHUMP00000033227; protein kinase, Y-linked |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
ORF Nucleotide Sequence
>RC210438 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCGCCCGGGCCGGCCCAGGCGGCCGCGGCGGAGAGCAACTCCCGAGAGGTGACGGAGGATGCCG CCGACTGGGCGCCCGCGCTCTGCCCCAGCCCCGAGGCGCGGTCGCCGGAGGCGCCTGCCTACCGCCTGCA GGACTGCGACGCGCTGGTCACCATGGGCACTGGGACGTTCGGGCGGGTGCACCTGGTGAAGGAGAAGACA GCCAAGCATTTCTTCGCCCTCAAGGTGATGAGCATTCCCGACGTCATCCGCCGGAAGCAGGAGCAGCACG TGCACAATGAGAAGTCTGTCCTGAAGGAAGTCAGCCACCCGTTCCTCATCAGGCTGTTCTGGACGTGGCA TGAGGAGCGCTTCCTCTACATGCTCATGGAGTATGTGCCGGGTGGCGAGCTCTTCAGCTACCTGCGCAAC CGGGGGCACTTCTCCAGCACCACGGGGCTCTTCTACTCTGCGGAGATCATCTGTGCCATTGAGTACCTGC ACTCCAAGGAGATCGTCTACAGGGATTTGAAGCCGGAGAACATCCTGCTGGATAGGGATGGTCACATCAA GCTCACGGACTTTGGGTTTGCCAAGAAGCTGGTAGACAGGACTTGGACCCTCTGTGGAACACCCGAGTAC CTAGCCCCCGAAGTCATTCAGAGCAAGGGCCACGGAAGGGCCGTGGACTGGTGGGCCCTCGGCATCCTGA TATTCGAGATGCTTTCGGGGTTTCCTCCATTTTTTGATGACAACCCGTTTGGCATTTATCAGAAAATTCT TGCAGGCAAACTATATTTCCCCAGACATTTGGATTTCCATGTAAAAACGGGGCGAATGATG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC210438 protein sequence
Red=Cloning site Green=Tags(s) MEAPGPAQAAAAESNSREVTEDAADWAPALCPSPEARSPEAPAYRLQDCDALVTMGTGTFGRVHLVKEKT AKHFFALKVMSIPDVIRRKQEQHVHNEKSVLKEVSHPFLIRLFWTWHEERFLYMLMEYVPGGELFSYLRN RGHFSSTTGLFYSAEIICAIEYLHSKEIVYRDLKPENILLDRDGHIKLTDFGFAKKLVDRTWTLCGTPEY LAPEVIQSKGHGRAVDWWALGILIFEMLSGFPPFFDDNPFGIYQKILAGKLYFPRHLDFHVKTGRMM TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
Chromatograms
![]() Sequencher program is needed, download here |
Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002760 |
ORF Size | 831 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
Shipping | Ambient |
Reference Data | |
RefSeq | NM_002760.3, NP_002751.1 |
RefSeq Size | 7216 bp |
RefSeq ORF | 833 bp |
Locus ID | 5616 |
Cytogenetics | Yp11.2 |
Domains | pkinase, S_TKc, TyrKc |
Protein Families | Druggable Genome, Protein Kinase |
MW | 31.7 kDa |
Summary | This gene is similar to the protein kinase, X-linked gene in the pseudoautosomal region of the X chromosome. The gene is classified as a transcribed pseudogene because it has lost a coding exon that results in all transcripts being candidates for nonsense-mediated decay (NMD) and unlikely to express a protein. Abnormal recombination between this gene and a related gene on chromosome X is a frequent cause of XX males and XY females. [provided by RefSeq, Jul 2010] |
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Product Manuals |
FAQs |
SDS |
SKU | Description | Size | Price | |
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RC210438L1 | Lenti ORF clone of Human protein kinase, Y-linked (PRKY), Myc-DDK-tagged | 10 ug |
$600.00
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RC210438L2 | Lenti ORF clone of Human protein kinase, Y-linked (PRKY), mGFP tagged | 10 ug |
$600.00
|
|
RC210438L3 | Lenti ORF clone of Human protein kinase, Y-linked (PRKY), Myc-DDK-tagged | 10 ug |
$600.00
|
|
RC210438L4 | Lenti ORF clone of Human protein kinase, Y-linked (PRKY), mGFP tagged | 10 ug |
$600.00
|
|
RG210438 | PRKY (tGFP-tagged) - Human protein kinase, Y-linked (PRKY) | 10 ug |
$489.00
MSRP
$500.00
MSRP
$500.00
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SC126856 | PRKY (untagged)-Human protein kinase, Y-linked (PRKY) | 10 ug |
$300.00
|
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SC323404 | PRKY (untagged)-Kinase deficient mutant (K78M) of Human protein kinase, Y-linked (PRKY) | 10 ug |
$300.00
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Citations
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.