GDNF Receptor alpha 2 (GFRA2) (NM_001495) Human Tagged ORF Clone

SKU RC208014

GFRA2 (Myc-DDK-tagged)-Human GDNF family receptor alpha 2 (GFRA2), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: GDNF Receptor alpha 2
• Synonyms: GDNFRB; NRTNR-ALPHA; NTNRA; RETL2; TRNR2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC208014 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGATCTTGGCAAACGTCTTCTGCCTCTTCTTCTTTCTAGACGAGACCCTCCGCTCTTTGGCCAGCCCTT; CCTCCCTGCAGGGCCCCGAGCTCCACGGCTGGCGCCCCCCAGTGGACTGTGTCCGGGCCAATGAGCTGTG; TGCCGCCGAATCCAACTGCAGCTCTCGCTACCGCACTCTGCGGCAGTGCCTGGCAGGCCGCGACCGCAAC; ACCATGCTGGCCAACAAGGAGTGCCAGGCGGCCTTGGAGGTCTTGCAGGAGAGCCCGCTGTACGACTGCC; GCTGCAAGCGGGGCATGAAGAAGGAGCTGCAGTGTCTGCAGATCTACTGGAGCATCCACCTGGGGCTGAC; CGAGGGTGAGGAGTTCTACGAAGCCTCCCCCTATGAGCCGGTGACCTCCCGCCTCTCGGACATCTTCAGG; CTTGCTTCAATCTTCTCAGGGACAGGGGCAGACCCGGTGGTCAGCGCCAAGAGCAACCATTGCCTGGATG; CTGCCAAGGCCTGCAACCTGAATGACAACTGCAAGAAGCTGCGCTCCTCCTACATCTCCATCTGCAACCG; CGAGATCTCGCCCACCGAGCGCTGCAACCGCCGCAAGTGCCACAAGGCCCTGCGCCAGTTCTTCGACCGG; GTGCCCAGCGAGTACACCTACCGCATGCTCTTCTGCTCCTGCCAAGACCAGGCGTGCGCTGAGCGCCGCC; GGCAAACCATCCTGCCCAGCTGCTCCTATGAGGACAAGGAGAAGCCCAACTGCCTGGACCTGCGTGGCGT; GTGCCGGACTGACCACCTGTGTCGGTCCCGGCTGGCCGACTTCCATGCCAATTGTCGAGCCTCCTACCAG; ACGGTCACCAGCTGCCCTGCGGACAATTACCAGGCGTGTCTGGGCTCTTATGCTGGCATGATTGGGTTTG; ACATGACACCTAACTATGTGGACTCCAGCCCCACTGGCATCGTGGTGTCCCCCTGGTGCAGCTGTCGTGG; CAGCGGGAACATGGAGGAGGAGTGTGAGAAGTTCCTCAGGGACTTCACCGAGAACCCATGCCTCCGGAAC; GCCATCCAGGCCTTTGGCAACGGCACGGACGTGAACGTGTCCCCAAAAGGCCCCTCGTTCCAGGCCACCC; AGGCCCCTCGGGTGGAGAAGACGCCTTCTTTGCCAGATGACCTCAGTGACAGTACCAGCTTGGGGACCAG; TGTCATCACCACCTGCACGTCTGTCCAGGAGCAGGGGCTGAAGGCCAACAACTCCAAAGAGTTAAGCATG; TGCTTCACAGAGCTCACGACAAATATCATCCCAGGGAGTAACAAGGTGATCAAACCTAACTCAGGCCCCA; GCAGAGCCAGACCGTCGGCTGCCTTGACCGTGCTGTCTGTCCTGATGCTGAAACTGGCCTTG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC208014 protein sequence; Red=Cloning site Green=Tags(s); ; MILANVFCLFFFLDETLRSLASPSSLQGPELHGWRPPVDCVRANELCAAESNCSSRYRTLRQCLAGRDRN; TMLANKECQAALEVLQESPLYDCRCKRGMKKELQCLQIYWSIHLGLTEGEEFYEASPYEPVTSRLSDIFR; LASIFSGTGADPVVSAKSNHCLDAAKACNLNDNCKKLRSSYISICNREISPTERCNRRKCHKALRQFFDR; VPSEYTYRMLFCSCQDQACAERRRQTILPSCSYEDKEKPNCLDLRGVCRTDHLCRSRLADFHANCRASYQ; TVTSCPADNYQACLGSYAGMIGFDMTPNYVDSSPTGIVVSPWCSCRGSGNMEEECEKFLRDFTENPCLRN; AIQAFGNGTDVNVSPKGPSFQATQAPRVEKTPSLPDDLSDSTSLGTSVITTCTSVQEQGLKANNSKELSM; CFTELTTNIIPGSNKVIKPNSGPSRARPSAALTVLSVLMLKLAL; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001495
• ORF Size: 1392 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001495.5
• RefSeq Size: 3376 bp
• RefSeq ORF: 1395 bp
• Locus ID: 2675
• UniProt ID: O00451
• Cytogenetics: 8p21.3
• Domains: GDNF
• Protein Families: Druggable Genome
• MW: 51.5 kDa
• Summary: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]