GDNF Receptor alpha 2 (GFRA2) (NM_001495) Human Untagged Clone

SKU SC108218

GFRA2 (untagged)-Human GDNF family receptor alpha 2 (GFRA2), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: GDNF Receptor alpha 2
• Synonyms: GDNFRB; NRTNR-ALPHA; NTNRA; RETL2; TRNR2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC108218 sequence for NM_001495 edited (data generated by NextGen Sequencing); ATGATCTTGGCAAACGTCTTCTGCCTCTTCTTCTTTCTAGACGAGACCCTCCGCTCTTTG; GCCAGCCCTTCCTCCCTGCAGGGCCCCGAGCTCCACGGCTGGCGCCCCCCAGTGGACTGT; GTCCGGGCCAATGAGCTGTGTGCCGCCGAATCCAACTGCAGCTCTCGCTACCGCACTCTG; CGGCAGTGCCTGGCAGGCCGCGACCGCAACACCATGCTGGCCAACAAGGAGTGCCAGGCG; GCCTTGGAGGTCTTGCAGGAGAGCCCGCTGTACGACTGCCGCTGCAAGCGGGGCATGAAG; AAGGAGCTGCAGTGTCTGCAGATCTACTGGAGCATCCACCTGGGGCTGACCGAGGGTGAG; GAGTTCTACGAAGCCTCCCCCTATGAGCCGGTGACCTCCCGCCTCTCGGACATCTTCAGG; CTTGCTTCAATCTTCTCAGGGACAGGGGCAGACCCGGTGGTCAGCGCCAAGAGCAACCAT; TGCCTGGATGCTGCCAAGGCCTGCAACCTGAATGACAACTGCAAGAAGCTGCGCTCCTCC; TACATCTCCATCTGCAACCGCGAGATCTCGCCCACCGAGCGCTGCAACCGCCGCAAGTGC; CACAAGGCCCTGCGCCAGTTCTTCGACCGGGTGCCCAGCGAGTACACCTACCGCATGCTC; TTCTGCTCCTGCCAAGACCAGGCGTGCGCTGAGCGCCGCCGGCAAACCATCCTGCCCAGC; TGCTCCTATGAGGACAAGGAGAAGCCCAACTGCCTGGACCTGCGTGGCGTGTGCCGGACT; GACCACCTCTGTCGGTCCCGGCTGGCCGACTTCCATGCCAATTGTCGAGCCTCCTACCAG; ACGGTCACCAGCTGCCCTGCGGACAATTACCAGGCGTGTCTGGGCTCTTATGCTGGCATG; ATTGGGTTTGACATGACACCTAACTATGTGGACTCCAGCCCCACTGGCATCGTGGTGTCC; CCCTGGTGCAGCTGTCGTGGCAGCGGGAACATGGAGGAGGAGTGTGAGAAGTTCCTCAGG; GACTTCACCGAGAACCCATGCCTCCGGAACGCCATCCAGGCCTTTGGCAACGGCACGGAC; GTGAACGTGTCCCCAAAAGGCCCCTCGTTCCAGGCCACCCAGGCCCCTCGGGTGGAGAAG; ACGCCTTCTTTGCCAGATGACCTCAGTGACAGTACCAGCTTGGGGACCAGTGTCATCACC; ACCTGCACGTCTGTCCAGGAGCAGGGGCTGAAGGCCAACAACTCCAAAGAGTTAAGCATG; TGCTTCACAGAGCTCACGACAAATATCATCCCAGGGAGTAACAAGGTGATCAAACCTAAC; TCAGGCCCCAGCAGAGCCAGACCGTCGGCTGCCTTGACCGTGCTGTCTGTCCTGATGCTG; AAACAGGCCTTGTAG; ; Clone variation with respect to NM_001495.4; 789 g=>c;1385 t=>a
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001495 unedited; GGTTCAAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCAGCCAGAG; GCGAGAATCGCAGGACTCGGGATCTTCATCGGGTGGACTAGCTGGGATCTCCGCATTGGA; TTTGGGGCTGATTACCACTGCTTGCCTATTATTATTGTTGTTGTTACTACTATTATTTTT; TTTTACCCAAGGGAGAAAGACAAAAAAACGGTGGGATTTATTTAACATGATCTTGGCAAA; CGTCTTCTGCCTCTTCTTCTTTCTACTCGAGACCCTCCGCTCTTTGGCCAGCCCTTCCTC; CCTGCAGGGCCCCGAGCTCCACGGCTGGCGCCCCCCAGTGGACTGTGTCCGGGCCAATGA; GCTGTGTGCCGCCGAATCCAACTGCAGCTCTCGCTACCGCACTCTGCGGCAGTGCCTGGC; AGGCCGCGACCGCAACACCATGCTGGCCAACAAGGAGTGCCAGGCGGCCTTGGAGGTCTT; GCAGGAGAGCCCGCTGTACGACTGCCGCTGCAAGCGGGGCATGAAGAAGGAGCTGCAGTG; TCTGCAGATCTACTGGAGCATCCACCTGGGGCTGACCGAGGGTGAGGAGTTCTACGAAGC; CTCCCCCTATGAGCCGGTGACCTCCCGCCTCTCGGACATCTTCAGGCTTGCTTCAATCTT; CTCAGGGACAGGGGCAGACCCGGTGGTCAGCGCCAAGAGCAACCATTGCCTGGATGCTGC; CAAGGCCTGNCACCTGAATGACAACTGCAAATAAGCTGCGCTCCTTCTACATCTNCATCT; GCAACCGCGAGATCTCGCCCACCGAGCGCTGCAACCGCCGCCAGTGCCACAAGGCCCTGC; GCCAGTTCTTTGACCGGGTGCCCAGCGAGTACACCTACCGCATGCTCTTCTGCTCCTGCC; AGAACAGCCGTGCGCTGAGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001495 unedited; TACTATGNACGCGGCCGCAATCTATGATCGGTTTTTTTTTTTTTTTTTTTTCAGCTTGCT; TCCTAACACGTTTATTGGAGCCTTGGTTAAGCTTGGATGCGGATCAGGAGCCCCCACCAG; AGCCCAGCCAGGCTGACTGGGGCAGGGCACTGCGGGGCCAATGTCCTTTTTCCCCATTAT; AGACAACAGTATTAAAAAAAATAAACTTTACAATAATACATTTTCGCTCATCTGTTGGGG; TATTATTTCCATAGTTTTGTTGCTTAAAAAAAGGAAAGAAAGGGGAAACCACACAATATA; TGTATATGGATGTGTATATACATATATGTTAGGAGAGGAGCCCCGGTATGTACATACAGT; TGACGTACGTCTAGGAAATGAGGAAAGAGAAAGGGGAAAGGAGGAGGAGGGAGAAGACAG; CAGGAAAGCTTGAGGGCCCACGGTGCTGCAGACACACACCCGGCTGTGGTCTCTCCCCTC; CCTCTCCCCAAAGGGAGGCCTGCCAAAGGACCCACGAGAAAGCAAAGAAGCCAACAAAGG; ACCGTTTCTCTCTGACTTCAAAATTTCTGCCACCGAGAAAGAAGCTGGTGCCCAAGTTTT; AATGCCTCGCCACAAGGGCTCTCCCAGGCAGGCTGAGCTCTCAAGCCCTCCTGGAGCTGG; CCCCCAGCCCCAACACTCCGTGCTACAGTCAGGGGGGAGCCCAGCACGGTGCGAACCCCC; TCCACCGCCATCCGTGGGGAAGTTCCCACACCAGCTGCAGACCCACCAGCCCCACCCGAG; GGCAGAGAGGGAGGGCAAAACCATCTCCATCCTTGAAGCCAAAGGCTGAAGGTGGACCCT; CATGGCAGCCCCGCTGCCCCGCTGACGATGCCCCTGCCACCCCCAGNGGGTCCCAAATGG; GCACCTGGGGATCACCTGGTTGTCCTGCCCCAACCAGCTTAGGCAGNCCATGATCCTTCG; TTG;
• Restriction Sites: NotI-NotI
• ACCN: NM_001495
• Insert Size: 3090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001495.4, NP_001486.4
• RefSeq Size: 3376 bp
• RefSeq ORF: 1395 bp
• Locus ID: 2675
• UniProt ID: O00451
• Cytogenetics: 8p21.3
• Domains: GDNF
• Protein Families: Druggable Genome
• Summary: Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. The protein encoded by this gene is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol(GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This encoded protein acts preferentially as a receptor for NTN compared to its other family member, GDNF family receptor alpha 1. This gene is a candidate gene for RET-associated diseases. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]; Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).