Intrinsic Factor (GIF) (NM_005142) Human Tagged ORF Clone

SKU
RC207777
GIF (Myc-DDK-tagged)-Human gastric intrinsic factor (vitamin B synthesis) (GIF)
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$457.00
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Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol Intrinsic Factor
Synonyms GIF; IF; IFMH; INF; TCN3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC207777 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCTGGTTTGCCCTCTACCTCCTGAGCCTTCTCTGGGCTACAGCTGGGACTAGTACCCAGACCCAGA
GTTCATGCTCCGTTCCCTCAGCACAGGAGCCCTTGGTCAATGGAATACAAGTACTCATGGAGAACTCGGT
GACTTCATCAGCCTACCCAAACCCCAGCATCCTGATTGCCATGAATCTGGCCGGAGCCTACAACTTGAAG
GCCCAGAAGCTCCTGACTTACCAGCTCATGTCCAGCGACAACAACGATCTAACCATTGGGCAGCTCGGCC
TCACCATCATGGCCCTCACCTCCTCCTGCCGAGACCCTGGGGATAAAGTATCCATTCTACAAAGACAAAT
GGAGAACTGGGCACCTTCCAGCCCCAACGCTGAAGCATCAGCCTTCTATGGGCCCAGTCTAGCGATCTTG
GCACTGTGCCAGAAGAACTCTGAGGCGACCTTGCCGATAGCCGTCCGCTTTGCCAAGACCCTGCTGGCCA
ACTCCTCTCCCTTCAATGTAGACACAGGAGCAATGGCAACCTTGGCTCTGACCTGTATGTACAACAAGAT
CCCTGTAGGTTCAGAGGAAGGTTACAGATCCCTGTTTGGTCAGGTACTAAAGGATATTGTGGAGAAAATC
AGCATGAAGATCAAAGATAATGGCATCATTGGAGACATCTACAGTACTGGCCTCGCCATGCAGGCTCTCT
CTGTAACACCTGAGCCATCTAAAAAGGAATGGAACTGCAAGAAGACTACGGATATGATACTCAATGAGAT
TAAGCAGGGGAAATTCCACAACCCCATGTCCATTGCTCAAATCCTCCCTTCCCTGAAAGGCAAGACATAC
CTAGATGTGCCCCAGGTCACTTGTAGTCCTGATCATGAGGTACAACCAACTCTACCCAGCAACCCTGGCC
CTGGCCCCACCTCTGCATCTAACATCACTGTCATATACACCATAAATAACCAGCTGAGGGGGGTTGAGCT
GCTCTTCAACGAGACCATCAATGTTAGTGTGAAAAGTGGGTCAGTGTTACTTGTTGTCCTAGAGGAAGCA
CAGCGCAAAAATCCTATGTTCAAATTTGAAACCACAATGACATCTTGGGGCCTTGTCGTCTCTTCTATCA
ACAATATCGCGGAAAATGTTAATCACAAGACATACTGGCAGTTTCTTAGTGGTGTAACACCTTTGAATGA
AGGGGTTGCTGACTACATACCCTTCAACCACGAGCACATCACAGCCAATTTCACACAGTAC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
Protein Sequence
>RC207777 protein sequence
Red=Cloning site Green=Tags(s)

MAWFALYLLSLLWATAGTSTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLK
AQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAIL
ALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKI
SMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTY
LDVPQVTCSPDHEVQPTLPSNPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEA
QRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN NM_005142
ORF Size 1251 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_005142.3
RefSeq Size 1536 bp
RefSeq ORF 1254 bp
Locus ID 2694
UniProt ID P27352
Cytogenetics 11q12.1
Protein Families Secreted Protein
MW 45.4 kDa
Summary This gene is a member of the cobalamin transport protein family. It encodes a glycoprotein secreted by parietal cells of the gastric mucosa and is required for adequate absorption of vitamin B12. Vitamin B12 is necessary for erythrocyte maturation and mutations in this gene may lead to congenital pernicious anemia. [provided by RefSeq, Jul 2008]
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SKU Description Size Price
RC207777L1 Lenti ORF clone of Human gastric intrinsic factor (vitamin B synthesis) (GIF), Myc-DDK-tagged 10 ug
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RC207777L3 Lenti ORF clone of Human gastric intrinsic factor (vitamin B synthesis) (GIF), Myc-DDK-tagged 10 ug
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RC207777L4 Lenti ORF clone of Human gastric intrinsic factor (vitamin B synthesis) (GIF), mGFP tagged 10 ug
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Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.