Human Intrinsic Factor (GIF) (NM_005142) AAV Particle

SKU RC207777A1V

AAV ORF Particles, serotype AAV-2, GIF (Myc-DDK-tagged)-Human gastric intrinsic factor (vitamin B synthesis) (GIF), 250ul, >10^13 GC/mL

Product Data

• Tag: Myc-DDK
• Target Symbol: Intrinsic Factor
• Synonyms: GIF; IF; IFMH; INF; TCN3
• Vector: pAAV-AC-Myc-DDK
• Mammalian Cell Selection: None
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207777 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTGGTTTGCCCTCTACCTCCTGAGCCTTCTCTGGGCTACAGCTGGGACTAGTACCCAGACCCAGA; GTTCATGCTCCGTTCCCTCAGCACAGGAGCCCTTGGTCAATGGAATACAAGTACTCATGGAGAACTCGGT; GACTTCATCAGCCTACCCAAACCCCAGCATCCTGATTGCCATGAATCTGGCCGGAGCCTACAACTTGAAG; GCCCAGAAGCTCCTGACTTACCAGCTCATGTCCAGCGACAACAACGATCTAACCATTGGGCAGCTCGGCC; TCACCATCATGGCCCTCACCTCCTCCTGCCGAGACCCTGGGGATAAAGTATCCATTCTACAAAGACAAAT; GGAGAACTGGGCACCTTCCAGCCCCAACGCTGAAGCATCAGCCTTCTATGGGCCCAGTCTAGCGATCTTG; GCACTGTGCCAGAAGAACTCTGAGGCGACCTTGCCGATAGCCGTCCGCTTTGCCAAGACCCTGCTGGCCA; ACTCCTCTCCCTTCAATGTAGACACAGGAGCAATGGCAACCTTGGCTCTGACCTGTATGTACAACAAGAT; CCCTGTAGGTTCAGAGGAAGGTTACAGATCCCTGTTTGGTCAGGTACTAAAGGATATTGTGGAGAAAATC; AGCATGAAGATCAAAGATAATGGCATCATTGGAGACATCTACAGTACTGGCCTCGCCATGCAGGCTCTCT; CTGTAACACCTGAGCCATCTAAAAAGGAATGGAACTGCAAGAAGACTACGGATATGATACTCAATGAGAT; TAAGCAGGGGAAATTCCACAACCCCATGTCCATTGCTCAAATCCTCCCTTCCCTGAAAGGCAAGACATAC; CTAGATGTGCCCCAGGTCACTTGTAGTCCTGATCATGAGGTACAACCAACTCTACCCAGCAACCCTGGCC; CTGGCCCCACCTCTGCATCTAACATCACTGTCATATACACCATAAATAACCAGCTGAGGGGGGTTGAGCT; GCTCTTCAACGAGACCATCAATGTTAGTGTGAAAAGTGGGTCAGTGTTACTTGTTGTCCTAGAGGAAGCA; CAGCGCAAAAATCCTATGTTCAAATTTGAAACCACAATGACATCTTGGGGCCTTGTCGTCTCTTCTATCA; ACAATATCGCGGAAAATGTTAATCACAAGACATACTGGCAGTTTCTTAGTGGTGTAACACCTTTGAATGA; AGGGGTTGCTGACTACATACCCTTCAACCACGAGCACATCACAGCCAATTTCACACAGTAC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207777 protein sequence; Red=Cloning site Green=Tags(s); ; MAWFALYLLSLLWATAGTSTQTQSSCSVPSAQEPLVNGIQVLMENSVTSSAYPNPSILIAMNLAGAYNLK; AQKLLTYQLMSSDNNDLTIGQLGLTIMALTSSCRDPGDKVSILQRQMENWAPSSPNAEASAFYGPSLAIL; ALCQKNSEATLPIAVRFAKTLLANSSPFNVDTGAMATLALTCMYNKIPVGSEEGYRSLFGQVLKDIVEKI; SMKIKDNGIIGDIYSTGLAMQALSVTPEPSKKEWNCKKTTDMILNEIKQGKFHNPMSIAQILPSLKGKTY; LDVPQVTCSPDHEVQPTLPSNPGPGPTSASNITVIYTINNQLRGVELLFNETINVSVKSGSVLLVVLEEA; QRKNPMFKFETTMTSWGLVVSSINNIAENVNHKTYWQFLSGVTPLNEGVADYIPFNHEHITANFTQY; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Species: Human
• Serotype: AAV-2
• ACCN: NM_005142
• ORF Size: 1251 bp
• Buffer: PBS with 0.001% Pluronic F68
• Stability: AAV is stable for 1 year when stored at -80°C (long-term storage) or 2-3 weeks when stored at -20°C (short-term storage). Thaw the vial of AAV on ice prior to use and keep it on ice during the experiment. Thawed AAV can be stored at 4°C for 1-2 weeks. Whenever possible, particles should be aliquoted into single use portions to avoid repeated freeze/thaw cycles. Please aliquot at least 10ul per tube and use low protein binding tubes to avoid loss of virus.
• Shipping: Dry Ice

Reference Data

• RefSeq: NM_005142.2
• RefSeq Size: 1536 bp
• RefSeq ORF: 1254 bp
• Locus ID: 2694
• UniProt ID: P27352
• Cytogenetics: 11q12.1
• MW: 45.4 kDa