Phosphorylase B (PHKB) (NM_001031835) Human Tagged ORF Clone

SKU RC207611

PHKB (Myc-DDK-tagged)-Human phosphorylase kinase, beta (PHKB), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Phosphorylase B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC207611 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTGCTCACCTGATGCAGTCGTCTCTCCGTCTTCCGCTTTCTTAAGGTCTGGCTCAGTTTATGAAC; CTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTCTGGGATAAGTTGGACCATTATTACAG; AATTGTCAAGTCAACATTGCTGCTGTATCAAAGTCCAACTACCGGTCTCTTTCCCACTAAAACATGCGGT; GGTGACCAGAAGGCCAAGATCCAGGACAGCCTATACTGCGCTGCTGGGGCCTGGGCTTTGGCTCTTGCAT; ACAGGCGAATTGATGACGACAAGGGAAGGACCCATGAGCTGGAGCACTCAGCTATAAAATGCATGAGAGG; AATTCTCTACTGCTATATGCGTCAGGCCGATAAGGTCCAGCAGTTTAAGCAGGATCCACGCCCAACAACA; TGTCTTCACTCTGTTTTCAATGTGCATACAGGAGATGAGTTGCTTTCCTATGAGGAATATGGTCATCTTC; AGATAAATGCAGTGTCACTTTATCTCCTTTACCTTGTGGAAATGATTTCCTCAGGACTCCAGATTATCTA; CAACACTGATGAGGTCTCTTTTATTCAAAACCTTGTATTTTGTGTGGAAAGAGTTTACCGTGTGCCTGAC; TTTGGTGTCTGGGAAAGAGGAAGCAAATATAATAATGGCAGCACAGAGCTACATTCGAGCTCGGTTGGTT; TAGCAAAAGCAGCTCTAGAAGCAATTAATGGATTCAACCTTTTTGGCAACCAGGGCTGTTCGTGGTCAGT; TATATTTGTGGATCTCGATGCTCACAATCGCAACAGGCAAACTTTGTGCTCGCTGTTACCCAGAGAATCA; AGATCACATAATACAGATGCTGCCCTGCTCCCCTGCATCAGTTATCCTGCATTTGCCCTGGATGATGAAG; TTCTTTTTAGCCAGACACTTGATAAAGTGGTTAGAAAATTAAAAGGAAAATATGGATTTAAACGTTTCTT; GAGAGATGGGTATAGAACATCATTGGAAGATCCCAACAGATGCTACTACAAGCCAGCTGAAATTAAGCTA; TTTGATGGCATTGAATGTGAATTTCCCATATTTTTCCTTTATATGATGATTGATGGAGTTTTTAGAGGCA; ATCCTAAGCAAGTACAGGAATATCAGGATCTTTTGACTCCAGTACTTCATCATACCACAGAAGGATATCC; TGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAAAAAAATAACCCTGGTAGTCAA; AAACGATTTCCTAGCAACTGTGGCCGTGATGGAAAACTGTTTCTTTGGGGACAAGCACTTTATATCATCG; CAAAACTCCTGGCTGATGAACTTATTAGTCCTAAAGACATTGATCCTGTCCAGCGCTATGTCCCACTAAA; GGATCAACGTAACGTGAGCATGAGGTTTTCCAATCAGGGCCCACTGGAAAATGACTTGGTAGTTCATGTG; GCACTTATAGCAGAAAGCCAAAGACTTCAAGTTTTTCTGAACACATATGGTATTCAAACTCAAACTCCTC; AACAAGTAGAACCCATTCAGATATGGCCTCAGCAGGAGCTTGTGAAAGCTTATTTGCAGCTGGGTATCAA; TGAAAAGTTAGGACTCTCTGGAAGGCCAGACAGGCCCATTGGCTGCCTCGGGACATCAAAGATTTATCGC; ATTCTAGGAAAGACTGTGGTTTGTTACCCGATTATTTTCGACCTAAGTGATTTCTACATGTCTCAGGATG; TTTTCCTGCTGATAGATGACATAAAGAATGCGCTGCAGTTCATTAAACAATATTGGAAAATGCATGGACG; TCCACTTTTCCTTGTTCTCATCCGGGAAGACAATATAAGAGGTAGCCGGTTCAACCCCATATTAGATATG; CTGGCAGCCCTTAAAAAAGGAATAATTGGAGGAGTCAAAGTTCATGTGGATCGTCTACAGACACTAATAT; CTGGAGCTGTGGTAGAACAACTTGATTTCCTACGAATCAGTGACACAGAAGAGCTTCCAGAATTTAAGAG; TTTTGAGGAACTAGAACCTCCCAAACATTCAAAAGTCAAACGGCAAAGCAGCACCCCTAGTGCTCCTGAA; CTGGGACAGCAGCCGGATGTCAACATTAGTGAATGGAAGGACAAACCCACCCATGAAATTCTTCAAAAAC; TGAATGATTGCAGTTGTCTGGCTAGCCAAGCCATCCTGCTGGGTATACTGCTCAAAAGAGAAGGCCCCAA; CTTCATCACAAAGGAAGGTACCGTTTCTGATCACATTGAGAGAGTCTATAGAAGAGCTGGCAGCCAAAAA; CTTTGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTAAGTAAAGTAGTGGACAGCCTGGCCCCATCCATTA; CTAATGTTTTAGTGCAGGGCAAACAGGTAACTCTGGGTGCCTTTGGGCATGAAGAAGAAGTTATCTCTAA; TCCTTTGTCTCCAAGAGTGATTCAAAACATCATCTATTATAAGTGTAACACCCATGATGAGAGGGAAGCG; GTCATTCAGCAAGAACTGGTCATCCATATTGGCTGGATCATCTCCAATAACCCTGAGTTATTCAGTGGCA; TGCTGAAAATACGAATCGGGTGGATCATCCATGCCATGGAGTATGAACTTCAGATCCGTGGCGGAGACAA; GCCAGCCTTGGACTTGTATCAGCTGTCACCTAGTGAAGTTAAACAGCTTCTGCTGGATATTCTGCAGCCT; CAACAGAATGGAAGATGTTGGCTGAACAGGCGTCAGATCGATGGGTCTTTGAATAGAACTCCCACCGGGT; TCTATGACCGAGTGTGGCAGATTCTGGAGCGCACGCCCAATGGGATCATTGTTGCTGGGAAGCATTTGCC; TCAGCAACCAACCCTGTCAGATATGACCATGTATGAGATGAATTTCTCTCTCCTTGTTGAAGACACGTTG; GGAAATATTGACCAGCCACAGTACAGACAGATCGTTGTAGAGTTACTTATGGTTGTATCCATTGTACTGG; AAAGAAACCCCGAGCTAGAATTTCAAGACAAAGTAGATCTAGACAGACTGGTCAAAGAAGCATTTAATGA; ATTTCAAAAAGATCAGAGTCGGCTAAAGGAAATTGAAAAACAAGATGACATGACTTCCTTTTACAACACT; CCTCCCCTGGGAAAAAGAGGAACATGCAGCTATTTGACAAAGGCGGTGATGAATCTGCTGCTGGAAGGAG; AAGTCAAGCCAAACAATGATGACCCGTGTCTGATTAGC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC207611 protein sequence; Red=Cloning site Green=Tags(s); ; MACSPDAVVSPSSAFLRSGSVYEPLKSINLPRPDNETLWDKLDHYYRIVKSTLLLYQSPTTGLFPTKTCG; GDQKAKIQDSLYCAAGAWALALAYRRIDDDKGRTHELEHSAIKCMRGILYCYMRQADKVQQFKQDPRPTT; CLHSVFNVHTGDELLSYEEYGHLQINAVSLYLLYLVEMISSGLQIIYNTDEVSFIQNLVFCVERVYRVPD; FGVWERGSKYNNGSTELHSSSVGLAKAALEAINGFNLFGNQGCSWSVIFVDLDAHNRNRQTLCSLLPRES; RSHNTDAALLPCISYPAFALDDEVLFSQTLDKVVRKLKGKYGFKRFLRDGYRTSLEDPNRCYYKPAEIKL; FDGIECEFPIFFLYMMIDGVFRGNPKQVQEYQDLLTPVLHHTTEGYPVVPKYYYVPADFVEYEKNNPGSQ; KRFPSNCGRDGKLFLWGQALYIIAKLLADELISPKDIDPVQRYVPLKDQRNVSMRFSNQGPLENDLVVHV; ALIAESQRLQVFLNTYGIQTQTPQQVEPIQIWPQQELVKAYLQLGINEKLGLSGRPDRPIGCLGTSKIYR; ILGKTVVCYPIIFDLSDFYMSQDVFLLIDDIKNALQFIKQYWKMHGRPLFLVLIREDNIRGSRFNPILDM; LAALKKGIIGGVKVHVDRLQTLISGAVVEQLDFLRISDTEELPEFKSFEELEPPKHSKVKRQSSTPSAPE; LGQQPDVNISEWKDKPTHEILQKLNDCSCLASQAILLGILLKREGPNFITKEGTVSDHIERVYRRAGSQK; LWSVVRRAASLLSKVVDSLAPSITNVLVQGKQVTLGAFGHEEEVISNPLSPRVIQNIIYYKCNTHDEREA; VIQQELVIHIGWIISNNPELFSGMLKIRIGWIIHAMEYELQIRGGDKPALDLYQLSPSEVKQLLLDILQP; QQNGRCWLNRRQIDGSLNRTPTGFYDRVWQILERTPNGIIVAGKHLPQQPTLSDMTMYEMNFSLLVEDTL; GNIDQPQYRQIVVELLMVVSIVLERNPELEFQDKVDLDRLVKEAFNEFQKDQSRLKEIEKQDDMTSFYNT; PPLGKRGTCSYLTKAVMNLLLEGEVKPNNDDPCLIS; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001031835
• ORF Size: 3258 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001031835.1, NP_001027005.1
• RefSeq Size: 5603 bp
• RefSeq ORF: 3261 bp
• Locus ID: 5257
• UniProt ID: Q93100
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• MW: 123.8 kDa
• Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010]