Phosphorylase B (PHKB) (NM_001031835) Human Untagged Clone

SKU SC302607

PHKB (untagged)-Human phosphorylase kinase, beta (PHKB), transcript variant 2

Product Data

• Type: Human Untagged Clone
• Target Symbol: Phosphorylase B
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC302607 representing NM_001031835.; Blue=Insert sequence Red=Cloning site Green=Tag(s); ; GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG; GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC; ATGGCCTGCTCACCTGATGCAGTCGTCTCTCCGTCTTCCGCTTTCTTAAGGTCTGGCTCAGTTTATGAA; CCTCTTAAAAGCATTAATCTTCCAAGACCTGATAATGAAACTCTCTGGGATAAGTTGGACCATTATTAC; AGAATTGTCAAGTCAACATTGCTGCTGTATCAAAGTCCAACTACCGGTCTCTTTCCCACTAAAACATGC; GGTGGTGACCAGAAGGCCAAGATCCAGGACAGCCTATACTGCGCTGCTGGGGCCTGGGCTTTGGCTCTT; GCATACAGGCGAATTGATGACGACAAGGGAAGGACCCATGAGCTGGAGCACTCAGCTATAAAATGCATG; AGAGGAATTCTCTACTGCTATATGCGTCAGGCCGATAAGGTCCAGCAGTTTAAGCAGGATCCACGCCCA; ACAACATGTCTTCACTCTGTTTTCAATGTGCATACAGGAGATGAGTTGCTTTCCTATGAGGAATATGGT; CATCTTCAGATAAATGCAGTGTCACTTTATCTCCTTTACCTTGTGGAAATGATTTCCTCAGGACTCCAG; ATTATCTACAACACTGATGAGGTCTCTTTTATTCAAAACCTTGTATTTTGTGTGGAAAGAGTTTACCGT; GTGCCTGACTTTGGTGTCTGGGAAAGAGGAAGCAAATATAATAATGGCAGCACAGAGCTACATTCGAGC; TCGGTTGGTTTAGCAAAAGCAGCTCTAGAAGCAATTAATGGATTCAACCTTTTTGGCAACCAGGGCTGT; TCGTGGTCAGTTATATTTGTGGATCTCGATGCTCACAATCGCAACAGGCAAACTTTGTGCTCGCTGTTA; CCCAGAGAATCAAGATCACATAATACAGATGCTGCCCTGCTCCCCTGCATCAGTTATCCTGCATTTGCC; CTGGATGATGAAGTTCTTTTTAGCCAGACACTTGATAAAGTGGTTAGAAAATTAAAAGGAAAATATGGA; TTTAAACGTTTCTTGAGAGATGGGTATAGAACATCATTGGAAGATCCCAACAGATGCTACTACAAGCCA; GCTGAAATTAAGCTATTTGATGGCATTGAATGTGAATTTCCCATATTTTTCCTTTATATGATGATTGAT; GGAGTTTTTAGAGGCAATCCTAAGCAAGTACAGGAATATCAGGATCTTTTGACTCCAGTACTTCATCAT; ACCACAGAAGGATATCCTGTTGTACCAAAGTACTATTATGTGCCAGCTGACTTTGTAGAATATGAAAAA; AATAACCCTGGTAGTCAAAAACGATTTCCTAGCAACTGTGGCCGTGATGGAAAACTGTTTCTTTGGGGA; CAAGCACTTTATATCATCGCAAAACTCCTGGCTGATGAACTTATTAGTCCTAAAGACATTGATCCTGTC; CAGCGCTATGTCCCACTAAAGGATCAACGTAACGTGAGCATGAGGTTTTCCAATCAGGGCCCACTGGAA; AATGACTTGGTAGTTCATGTGGCACTTATAGCAGAAAGCCAAAGACTTCAAGTTTTTCTGAACACATAT; GGTATTCAAACTCAAACTCCTCAACAAGTAGAACCCATTCAGATATGGCCTCAGCAGGAGCTTGTGAAA; GCTTATTTGCAGCTGGGTATCAATGAAAAGTTAGGACTCTCTGGAAGGCCAGACAGGCCCATTGGCTGC; CTCGGGACATCAAAGATTTATCGCATTCTAGGAAAGACTGTGGTTTGTTACCCGATTATTTTCGACCTA; AGTGATTTCTACATGTCTCAGGATGTTTTCCTGCTGATAGATGACATAAAGAATGCGCTGCAGTTCATT; AAACAATATTGGAAAATGCATGGACGTCCACTTTTCCTTGTTCTCATCCGGGAAGACAATATAAGAGGT; AGCCGGTTCAACCCCATATTAGATATGCTGGCAGCCCTTAAAAAAGGAATAATTGGAGGAGTCAAAGTT; CATGTGGATCGTCTACAGACACTAATATCTGGAGCTGTGGTAGAACAACTTGATTTCCTACGAATCAGT; GACACAGAAGAGCTTCCAGAATTTAAGAGTTTTGAGGAACTAGAACCTCCCAAACATTCAAAAGTCAAA; CGGCAAAGCAGCACCCCTAGTGCTCCTGAACTGGGACAGCAGCCGGATGTCAACATTAGTGAATGGAAG; GACAAACCCACCCATGAAATTCTTCAAAAACTGAATGATTGCAGTTGTCTGGCTAGCCAAGCCATCCTG; CTGGGTATACTGCTCAAAAGAGAAGGCCCCAACTTCATCACAAAGGAAGGTACCGTTTCTGATCACATT; GAGAGAGTCTATAGAAGAGCTGGCAGCCAAAAACTTTGGTCGGTTGTACGCCGTGCAGCAAGTCTTTTA; AGTAAAGTAGTGGACAGCCTGGCCCCATCCATTACTAATGTTTTAGTGCAGGGCAAACAGGTAACTCTG; GGTGCCTTTGGGCATGAAGAAGAAGTTATCTCTAATCCTTTGTCTCCAAGAGTGATTCAAAACATCATC; TATTATAAGTGTAACACCCATGATGAGAGGGAAGCGGTCATTCAGCAAGAACTGGTCATCCATATTGGC; TGGATCATCTCCAATAACCCTGAGTTATTCAGTGGCATGCTGAAAATACGAATCGGGTGGATCATCCAT; GCCATGGAGTATGAACTTCAGATCCGTGGCGGAGACAAGCCAGCCTTGGACTTGTATCAGCTGTCACCT; AGTGAAGTTAAACAGCTTCTGCTGGATATTCTGCAGCCTCAACAGAATGGAAGATGTTGGCTGAACAGG; CGTCAGATCGATGGGTCTTTGAATAGAACTCCCACCGGGTTCTATGACCGAGTGTGGCAGATTCTGGAG; CGCACGCCCAATGGGATCATTGTTGCTGGGAAGCATTTGCCTCAGCAACCAACCCTGTCAGATATGACC; ATGTATGAGATGAATTTCTCTCTCCTTGTTGAAGACACGTTGGGAAATATTGACCAGCCACAGTACAGA; CAGATCGTTGTAGAGTTACTTATGGTTGTATCCATTGTACTGGAAAGAAACCCCGAGCTAGAATTTCAA; GACAAAGTAGATCTAGACAGACTGGTCAAAGAAGCATTTAATGAATTTCAAAAAGATCAGAGTCGGCTA; AAGGAAATTGAAAAACAAGATGACATGACTTCCTTTTACAACACTCCTCCCCTGGGAAAAAGAGGAACA; TGCAGCTATTTGACAAAGGCGGTGATGAATCTGCTGCTGGAAGGAGAAGTCAAGCCAAACAATGATGAC; CCGTGTCTGATTAGCTAG; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT; TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC;
• Restriction Sites: SgfI-MluI
• ACCN: NM_001031835
• Insert Size: 3261 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_001031835.1
• RefSeq Size: 5603 bp
• RefSeq ORF: 3261 bp
• Locus ID: 5257
• UniProt ID: Q93100
• Cytogenetics: 16q12.1
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Insulin signaling pathway
• MW: 123.8 kDa
• Summary: Phosphorylase kinase is a polymer of 16 subunits, four each of alpha, beta, gamma and delta. The alpha subunit includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The beta subunit is the same in both the muscle and hepatic isoforms, encoded by this gene, which is a member of the phosphorylase b kinase regulatory subunit family. The gamma subunit also includes the skeletal muscle and hepatic isoforms, encoded by two different genes. The delta subunit is a calmodulin and can be encoded by three different genes. The gamma subunits contain the active site of the enzyme, whereas the alpha and beta subunits have regulatory functions controlled by phosphorylation. The delta subunit mediates the dependence of the enzyme on calcium concentration. Mutations in this gene cause glycogen storage disease type 9B, also known as phosphorylase kinase deficiency of liver and muscle. Alternatively spliced transcript variants encoding different isoforms have been identified in this gene. Two pseudogenes have been found on chromosomes 14 and 20, respectively.[provided by RefSeq, Feb 2010]; Transcript Variant: This variant (2) has an additional exon in the 5' region and an alternate exon in the 3' region, as compared to variant 1. The resulting isoform (b) is shorter and has an alternate N-terminus and a different segment in the C-terminal region, as compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.