SEPTIN5 (NM_002688) Human Tagged ORF Clone

SKU RC206831

SEPT5 (Myc-DDK-tagged)-Human septin 5 (SEPT5), transcript variant 1

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: SEPTIN5
• Synonyms: CDCREL; CDCREL-1; CDCREL1; H5; HCDCREL-1; PNUTL1; SEPT5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206831 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGAGCACAGGCCTGCGGTACAAGAGCAAGCTGGCGACCCCAGAGGACAAGCAGGACATTGACAAGCAGT; ACGTGGGCTTCGCCACACTGCCCAACCAGGTGCACCGCAAGTCGGTGAAGAAAGGCTTTGACTTCACACT; CATGGTGGCTGGTGAGTCAGGCCTGGGGAAGTCCACACTGGTCCACAGCCTCTTCCTGACAGACTTGTAC; AAGGACCGGAAGCTGCTCAGTGCTGAGGAGCGCATCAGCCAGACGGTAGAGATTCTAAAACACACGGTGG; ACATTGAGGAGAAGGGAGTCAAGCTGAAGCTCACCATCGTGGACACGCCGGGATTCGGGGACGCTGTCAA; CAACACCGAGTGCTGGAAGCCCATCACCGACTATGTGGACCAGCAGTTTGAGCAGTACTTCCGTGATGAG; AGCGGCCTCAACCGAAAGAACATCCAAGACAACCGAGTGCACTGCTGCCTATACTTCATCTCCCCCTTCG; GGCATGGGCTGCGGCCAGTGGATGTGGGTTTCATGAAGGCATTGCATGAGAAGGTCAACATCGTGCCTCT; CATCGCCAAAGCTGACTGTCTTGTCCCCAGTGAGATCCGGAAGCTGAAGGAGCGGATCCGGGAGGAGATT; GACAAGTTTGGGATCCATGTATACCAGTTCCCTGAGTGTGACTCGGACGAGGATGAGGACTTCAAGCAGC; AGGACCGGGAACTGAAGGAGAGCGCGCCCTTCGCCGTTATAGGCAGCAACACGGTGGTGGAGGCCAAGGG; GCAGCGGGTCCGGGGCCGACTGTACCCCTGGGGGATCGTGGAGGTGGAGAACCAGGCGCATTGCGACTTC; GTGAAGCTGCGCAACATGCTCATCCGCACGCATATGCACGACCTCAAGGACGTGACGTGCGACGTGCACT; ACGAGAACTACCGCGCGCACTGCATCCAGCAGATGACCAGCAAACTGACCCAGGACAGCCGCATGGAGAG; CCCCATCCCGATCCTGCCGCTGCCCACCCCGGACGCCGAGACTGAGAAGCTTATCAGGATGAAGGATGAG; GAACTGAGGCGCATGCAGGAGATGCTGCAGAGGATGAAGCAGCAGATGCAGGACCAG; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206831 protein sequence; Red=Cloning site Green=Tags(s); ; MSTGLRYKSKLATPEDKQDIDKQYVGFATLPNQVHRKSVKKGFDFTLMVAGESGLGKSTLVHSLFLTDLY; KDRKLLSAEERISQTVEILKHTVDIEEKGVKLKLTIVDTPGFGDAVNNTECWKPITDYVDQQFEQYFRDE; SGLNRKNIQDNRVHCCLYFISPFGHGLRPVDVGFMKALHEKVNIVPLIAKADCLVPSEIRKLKERIREEI; DKFGIHVYQFPECDSDEDEDFKQQDRELKESAPFAVIGSNTVVEAKGQRVRGRLYPWGIVEVENQAHCDF; VKLRNMLIRTHMHDLKDVTCDVHYENYRAHCIQQMTSKLTQDSRMESPIPILPLPTPDAETEKLIRMKDE; ELRRMQEMLQRMKQQMQDQ; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_002688
• ORF Size: 1107 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002688.6
• RefSeq Size: 2090 bp
• RefSeq ORF: 1110 bp
• Locus ID: 5413
• UniProt ID: Q99719
• Cytogenetics: 22q11.21
• Domains: GTP_CDC
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease
• MW: 42.8 kDa
• Summary: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]