SEPTIN5 (NM_002688) Human Untagged Clone

SKU SC118516

41522 (untagged)-Human septin 5 (SEPT5), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: SEPTIN5
• Synonyms: CDCREL; CDCREL-1; CDCREL1; H5; HCDCREL-1; PNUTL1; SEPT5
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118516 sequence for NM_002688 edited (data generated by NextGen Sequencing); ATGAGCACAGGCCTGCGGTACAAGAGCAAGCTGGCGACCCCAGAGGACAAGCAGGACATT; GACAAGCAGTACGTGGGCTTCGCCACACTGCCCAACCAGGTGCACCGCAAGTCGGTGAAG; AAAGGCTTTGACTTCACACTCATGGTGGCTGGTGAGTCAGGCCTGGGGAAGTCCACACTG; GTCCACAGCCTCTTCCTGACAGACTTGTACAAGGACCGGAAGCTGCTCAGTGCTGAGGAG; CGCATCAGCCAGACGGTAGAGATTCTAAAACACACGGTGGACATTGAGGAGAAGGGAGTC; AAGCTGAAGCTCACCATCGTGGACACGCCGGGATTCGGGGACGCTGTCAACAACACCGAG; TGCTGGAAGCCCATCACCGACTATGTGGACCAGCAGTTTGAGCAGTACTTCCGTGATGAG; AGCGGCCTCAACCGAAAGAACATCCAAGACAACCGAGTGCACTGCTGCCTATACTTCATC; TCCCCCTTCGGGCATGGGCTGCGGCCAGTGGATGTGGGTTTCATGAAGGCATTGCATGAG; AAGGTCAACATCGTGCCTCTCATCGCCAAAGCTGACTGTCTTGTCCCCAGTGAGATCCGG; AAGCTGAAGGAGCGGATCCGGGAGGAGATTGACAAGTTTGGGATCCATGTATACCAGTTC; CCTGAGTGTGACTCGGACGAGGATGAGGACTTCAAGCAGCAGGACCGGGAACTGAAGGAG; AGCGCGCCCTTCGCCGTTATAGGCAGCAACACGGTGGTGGAGGCCAAGGGGCAGCGGGTC; CGGGGCCGACTGTACCCCTGGGGGATCGTGGAGGTGGAGAACCAGGCGCATTGCGACTTC; GTGAAGCTGCGCAACATGCTCATCCGCACGCATATGCACGACCTCAAGGACGTGACGTGC; GACGTGCACTACGAGAACTACCGCGCGCACTGCATCCAGCAGATGACCAGCAAACTGACC; CAGGACAGCCGCATGGAGAGCCCCATCCCGATCCTGCCGCTGCCCACCCCGGACGCCGAG; ACTGAGAAGCTTATCAGGATGAAGGATGAGGAACTGAGGCGCATGCAGGAGATGCTGCAG; AGGATGAAGCAGCAGATGCAGGACCAGTGA; ; Clone variation with respect to NM_002688.5;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002688 unedited; CGGCCGGCGAATTCGCACGAGGGCGCGGAGGGGCCGCTCACCCCGCAGCCCGGCCTCGGC; CTCCGCCGCTTGTCGTCGCGCCCCGCCCGCGAGCCCGCCCCGCACGTCCCCCGCCGGCGG; CCACCATGAGCACAGGCCTGCGGTACAAGAGCAAGCTGGCGACCCCAGAGGACAAGCAGG; ACATTGACAAGCAGTACGTGGGCTTCGCCACACTGCCCAACCAGGTGCACCGCAAGTCGG; TGAAGAAAGGCTTTGACTTCACACTCATGGTGGCTGGTGAGTCAGGCCTGGGGAAGTCCA; CACTGGTCCACAGCCTCTTCCTGACAGACTTGTACAAGGACCGGAAGCTGCTCAGTGCTG; AGGAGCGCATCAGCCAGACGGTAGAGATTCTAAAACACACGGTGGACATTGAGGAGAAGG; GAGTCAAGCTGAAGCTCACCATCGTGGACACGCCGGGATTCGGGGACGCTGTCAACAACA; CCGAGTGCTGGAAGCCCATCACCGACTATGTGGACCAGCAGTTTGAGCAGTACTTCCGTG; ATGAGAGCGGCCTCAACCGAAAGAACATCCAAGACAACCGAGTGCACTGCTGCCTATACT; TCATCTCCCCCTTCGGGCATGGGCTGCGGCCAGTGATGTTGGGTTTCATGAAGGCATTGA; NTGAGAAGGTCAACATCGTGCCTCTCATCGCAAAGCTGACTGTCTTGTCCAAATTGGATC; CNGAAGCTGANGNANCGGATCCNGNAAGNGAATTGAACAGTTTTTGGGATCCATGTTTTA; CCCAGTCCCTTGATTGTGACTTCGACCAAGGATGAGGACTTCAAGCAGCAGACCGGGACT; GGAAGGAAACGCGCCTTTCCCGTATAGCGACACCGGGGGGGGGGAGC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002688 unedited; NATATCTTGCCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTT; TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGGCGGTCACCAACATTGTTCTCCTG; TCACCAAGACAGGACCCGGGTTTTACAACGGAAAAAAACGGGGCCTTGTGGGGGGGGAAG; GGATCATGCGGGGGGGAACATTGGGGGGCCTAAGCTTAAGATCCCGGCCCACCACAAGGG; GGCTGGGGAAAAAAGGCCTCCAGCGAGGGCCGGGGACCGCCCCCAAAGTTGCCCGGAGGG; GGGTTACCCCCCAAGGCCCCGGGGGCCCTCCAAGTCGGGCCGGGCTTTGGAAAAAGGGGG; TAAACGGTCACAACCCCCCTCCTTGGAACCTTTGGCCTCCTTTTGTTTGGGGGGCTCCCA; TTTGATTTAACCCAACCCTTCCCCCTAGGGGTTTGGGGATAAAGGAAAACTCGGCCCCCC; TCGACAAAAAATGGGGGCTCAACACTCCCTGGGGGACCCCCGGGAACAGGCAAAGGAGGG; CTCCCAAGTCCCTTTTGCACACTGGGGCCGGGAACAAACCCACGATCCTGGGAAACCTTT; TTTGCCTAACTTTGCCTGCCTGCCAAAACAAGGCCTGGGGGTTTTGGGTGGCTGGGACCC; CCCCGGGGGCGGGGGGAAAAGGCAAAGGGAGGCCGGATTTCTTGAAATTCCCGCCCCACT; TTCCCTCCTGTTCCCCCCAGGGCACAAAAGAGGCCACTGGGGGGGGGGGCTTCGGTCCAC; GGCCCCTTGGAACAAACAACATGACCTGGGAGGGGGGGGGGCCCCAAAATAAAATAAGGG; ACCTTGGGGGCTGGTGCCCCCGGTCTCCCGGGGGAGAACAAATCCGCGCTGTGTTCCAAG; GGGCCAAGGGCTCCCCGCAACAACCCGGTTTTCCCCCGCACCACACCGCCCCCCGTTATG; GGTTCTTCTTTGCG;
• Restriction Sites: NotI-NotI
• ACCN: NM_002688
• Insert Size: 2080 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002688.3, NP_002679.2
• RefSeq Size: 2080 bp
• RefSeq ORF: 1110 bp
• Locus ID: 5413
• UniProt ID: Q99719
• Cytogenetics: 22q11.21
• Domains: GTP_CDC
• Protein Families: Druggable Genome
• Protein Pathways: Parkinson's disease
• Summary: This gene is a member of the septin gene family of nucleotide binding proteins, originally described in yeast as cell division cycle regulatory proteins. Septins are highly conserved in yeast, Drosophila, and mouse and appear to regulate cytoskeletal organization. Disruption of septin function disturbs cytokinesis and results in large multinucleate or polyploid cells. This gene is mapped to 22q11, the region frequently deleted in DiGeorge and velocardiofacial syndromes. A translocation involving the MLL gene and this gene has also been reported in patients with acute myeloid leukemia. Alternative splicing results in multiple transcript variants. The presence of a non-consensus polyA signal (AACAAT) in this gene also results in read-through transcription into the downstream neighboring gene (GP1BB; platelet glycoprotein Ib), whereby larger, non-coding transcripts are produced. [provided by RefSeq, Dec 2010]; Transcript Variant: This variant (1) encodes the longer isoform (1).