SLC22A4 (NM_003059) Human Tagged ORF Clone

CAT#: RC206575

SLC22A4 (Myc-DDK-tagged)-Human solute carrier family 22 (organic cation/ergothioneine transporter), member 4 (SLC22A4)

Product Images

Circular map for RC206575

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  Western blot validation of overexpression lysate (Cat# LY401067) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206575 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC22A4
• Synonyms: DFNB60; OCTN1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206575 representing NM_003059
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGGGACTACGACGAGGTGATCGCCTTCCTGGGCGAGTGGGGGCCCTTCCAGCGCCTCATCTTCTTCC
  TGCTCAGCGCCAGCATCATCCCCAATGGCTTCAATGGTATGTCAGTCGTGTTCCTGGCGGGGACCCCGGA
  GCACCGCTGTCGAGTGCCGGACGCCGCGAACCTGAGCAGCGCCTGGCGCAACAACAGTGTCCCGCTGCGG
  CTGCGGGACGGCCGCGAGGTGCCCCACAGCTGCAGCCGCTACCGGCTCGCCACCATCGCCAACTTCTCGG
  CGCTCGGGCTGGAGCCGGGGCGCGACGTGGACCTGGGGCAGCTGGAGCAGGAGAGCTGCCTGGATGGCTG
  GGAGTTCAGCCAGGACGTCTACCTGTCCACCGTCGTGACCGAGTGGAATCTGGTGTGTGAGGACAACTGG
  AAGGTGCCCCTCACCACCTCCCTGTTCTTCGTAGGCGTGCTCCTCGGCTCCTTCGTGTCCGGGCAGCTGT
  CAGACAGGTTTGGCAGGAAGAACGTTCTCTTCGCAACCATGGCTGTACAGACTGGCTTCAGCTTCCTGCA
  GATTTTCTCCATCAGCTGGGAGATGTTCACTGTGTTATTTGTCATCGTGGGCATGGGCCAGATCTCCAAC
  TATGTGGTAGCCTTCATACTAGGAACAGAAATTCTTGGCAAGTCAGTTCGTATTATATTCTCTACATTAG
  GAGTGTGCACATTTTTTGCAGTTGGCTATATGCTGCTGCCACTGTTTGCTTACTTCATCAGAGACTGGCG
  GATGCTGCTGCTGGCGCTGACGGTGCCGGGAGTGCTGTGTGTCCCGCTGTGGTGGTTCATTCCTGAATCT
  CCCCGATGGCTGATATCCCAGAGAAGATTTAGAGAGGCTGAAGATATCATCCAAAAAGCTGCAAAAATGA
  ACAACATAGCTGTACCAGCAGTGATATTTGATTCTGTGGAGGAGCTAAATCCCCTGAAGCAGCAGAAAGC
  TTTCATTCTGGACCTGTTCAGGACTCGGAATATTGCCATAATGACCATTATGTCTTTGCTGCTATGGATG
  CTGACCTCAGTGGGTTACTTTGCTCTGTCTCTGGATGCTCCTAATTTACATGGAGATGCCTACCTGAACT
  GTTTCCTCTCTGCCTTGATTGAAATTCCAGCTTACATTACAGCCTGGCTGCTATTGCGAACCCTGCCCAG
  GCGTTATATCATAGCTGCAGTACTGTTCTGGGGAGGAGGTGTCCTTCTCTTCATTCAACTGGTACCTGTG
  GATTATTACTTCTTATCCATTGGTCTGGTCATGCTGGGAAAATTTGGGATCACCTCTGCTTTCTCCATGC
  TGTATGTCTTCACTGCTGAGCTCTACCCAACCCTGGTCAGGAACATGGCGGTGGGGGTCACATCCACGGC
  CTCCAGAGTGGGCAGCATCATTGCCCCCTACTTTGTTTACCTCGGTGCTTACAACAGAATGCTGCCCTAC
  ATCGTCATGGGTAGTCTGACTGTCCTGATTGGAATCCTCACCCTTTTTTTCCCTGAAAGTTTGGGAATGA
  CTCTTCCAGAAACCTTAGAGCAGATGCAGAAAGTGAAATGGTTCAGATCTGGGAAAAAAACAAGAGACTC
  AATGGAGACAGAAGAAAATCCCAAGGTTCTAATAACTGCATTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206575 representing NM_003059
  Red=Cloning site Green=Tags(s)
  
  MRDYDEVIAFLGEWGPFQRLIFFLLSASIIPNGFNGMSVVFLAGTPEHRCRVPDAANLSSAWRNNSVPLR
  LRDGREVPHSCSRYRLATIANFSALGLEPGRDVDLGQLEQESCLDGWEFSQDVYLSTVVTEWNLVCEDNW
  KVPLTTSLFFVGVLLGSFVSGQLSDRFGRKNVLFATMAVQTGFSFLQIFSISWEMFTVLFVIVGMGQISN
  YVVAFILGTEILGKSVRIIFSTLGVCTFFAVGYMLLPLFAYFIRDWRMLLLALTVPGVLCVPLWWFIPES
  PRWLISQRRFREAEDIIQKAAKMNNIAVPAVIFDSVEELNPLKQQKAFILDLFRTRNIAIMTIMSLLLWM
  LTSVGYFALSLDAPNLHGDAYLNCFLSALIEIPAYITAWLLLRTLPRRYIIAAVLFWGGGVLLFIQLVPV
  DYYFLSIGLVMLGKFGITSAFSMLYVFTAELYPTLVRNMAVGVTSTASRVGSIIAPYFVYLGAYNRMLPY
  IVMGSLTVLIGILTLFFPESLGMTLPETLEQMQKVKWFRSGKKTRDSMETEENPKVLITAF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003059
• ORF Size: 1653 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_003059.3
• RefSeq Size: 2214 bp
• RefSeq ORF: 1656 bp
• Locus ID: 6583
• UniProt ID: Q9H015
• Cytogenetics: 5q31.1
• Domains: sugar_tr
• Protein Families: Transmembrane
• MW: 62 kDa
• Gene Summary: Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]