SLC22A4 (NM_003059) Human Tagged ORF Clone

CAT#: RG206575

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  • TrueORF®

SLC22A4 (GFP-tagged) - Human solute carrier family 22 (organic cation/ergothioneine transporter), member 4 (SLC22A4)



  "NM_003059" in other vectors (6)

Reconstitution Protocol

USD 969.00


Availability*
In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag TurboGFP
Symbol SLC22A4
Synonyms DFNB60; OCTN1
Vector pCMV6-AC-GFP
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RG206575 representing NM_003059
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCGGGACTACGACGAGGTGATCGCCTTCCTGGGCGAGTGGGGGCCCTTCCAGCGCCTCATCTTCTTCC
TGCTCAGCGCCAGCATCATCCCCAATGGCTTCAATGGTATGTCAGTCGTGTTCCTGGCGGGGACCCCGGA
GCACCGCTGTCGAGTGCCGGACGCCGCGAACCTGAGCAGCGCCTGGCGCAACAACAGTGTCCCGCTGCGG
CTGCGGGACGGCCGCGAGGTGCCCCACAGCTGCAGCCGCTACCGGCTCGCCACCATCGCCAACTTCTCGG
CGCTCGGGCTGGAGCCGGGGCGCGACGTGGACCTGGGGCAGCTGGAGCAGGAGAGCTGCCTGGATGGCTG
GGAGTTCAGCCAGGACGTCTACCTGTCCACCGTCGTGACCGAGTGGAATCTGGTGTGTGAGGACAACTGG
AAGGTGCCCCTCACCACCTCCCTGTTCTTCGTAGGCGTGCTCCTCGGCTCCTTCGTGTCCGGGCAGCTGT
CAGACAGGTTTGGCAGGAAGAACGTTCTCTTCGCAACCATGGCTGTACAGACTGGCTTCAGCTTCCTGCA
GATTTTCTCCATCAGCTGGGAGATGTTCACTGTGTTATTTGTCATCGTGGGCATGGGCCAGATCTCCAAC
TATGTGGTAGCCTTCATACTAGGAACAGAAATTCTTGGCAAGTCAGTTCGTATTATATTCTCTACATTAG
GAGTGTGCACATTTTTTGCAGTTGGCTATATGCTGCTGCCACTGTTTGCTTACTTCATCAGAGACTGGCG
GATGCTGCTGCTGGCGCTGACGGTGCCGGGAGTGCTGTGTGTCCCGCTGTGGTGGTTCATTCCTGAATCT
CCCCGATGGCTGATATCCCAGAGAAGATTTAGAGAGGCTGAAGATATCATCCAAAAAGCTGCAAAAATGA
ACAACATAGCTGTACCAGCAGTGATATTTGATTCTGTGGAGGAGCTAAATCCCCTGAAGCAGCAGAAAGC
TTTCATTCTGGACCTGTTCAGGACTCGGAATATTGCCATAATGACCATTATGTCTTTGCTGCTATGGATG
CTGACCTCAGTGGGTTACTTTGCTCTGTCTCTGGATGCTCCTAATTTACATGGAGATGCCTACCTGAACT
GTTTCCTCTCTGCCTTGATTGAAATTCCAGCTTACATTACAGCCTGGCTGCTATTGCGAACCCTGCCCAG
GCGTTATATCATAGCTGCAGTACTGTTCTGGGGAGGAGGTGTCCTTCTCTTCATTCAACTGGTACCTGTG
GATTATTACTTCTTATCCATTGGTCTGGTCATGCTGGGAAAATTTGGGATCACCTCTGCTTTCTCCATGC
TGTATGTCTTCACTGCTGAGCTCTACCCAACCCTGGTCAGGAACATGGCGGTGGGGGTCACATCCACGGC
CTCCAGAGTGGGCAGCATCATTGCCCCCTACTTTGTTTACCTCGGTGCTTACAACAGAATGCTGCCCTAC
ATCGTCATGGGTAGTCTGACTGTCCTGATTGGAATCCTCACCCTTTTTTTCCCTGAAAGTTTGGGAATGA
CTCTTCCAGAAACCTTAGAGCAGATGCAGAAAGTGAAATGGTTCAGATCTGGGAAAAAAACAAGAGACTC
AATGGAGACAGAAGAAAATCCCAAGGTTCTAATAACTGCATTC


ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
>RG206575 representing NM_003059
Red=Cloning site Green=Tags(s)

MRDYDEVIAFLGEWGPFQRLIFFLLSASIIPNGFNGMSVVFLAGTPEHRCRVPDAANLSSAWRNNSVPLR
LRDGREVPHSCSRYRLATIANFSALGLEPGRDVDLGQLEQESCLDGWEFSQDVYLSTVVTEWNLVCEDNW
KVPLTTSLFFVGVLLGSFVSGQLSDRFGRKNVLFATMAVQTGFSFLQIFSISWEMFTVLFVIVGMGQISN
YVVAFILGTEILGKSVRIIFSTLGVCTFFAVGYMLLPLFAYFIRDWRMLLLALTVPGVLCVPLWWFIPES
PRWLISQRRFREAEDIIQKAAKMNNIAVPAVIFDSVEELNPLKQQKAFILDLFRTRNIAIMTIMSLLLWM
LTSVGYFALSLDAPNLHGDAYLNCFLSALIEIPAYITAWLLLRTLPRRYIIAAVLFWGGGVLLFIQLVPV
DYYFLSIGLVMLGKFGITSAFSMLYVFTAELYPTLVRNMAVGVTSTASRVGSIIAPYFVYLGAYNRMLPY
IVMGSLTVLIGILTLFFPESLGMTLPETLEQMQKVKWFRSGKKTRDSMETEENPKVLITAF

TRTRPLE - GFP Tag - V
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_003059
ORF Size 1653 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_003059.2, NP_003050.2
RefSeq Size 2214 bp
RefSeq ORF 1656 bp
Locus ID 6583
UniProt ID Q9H015
Cytogenetics 5q31.1
Domains sugar_tr
Protein Families Transmembrane
Gene Summary Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]

Other Versions

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