SLC22A4 (NM_003059) Human Untagged Clone

CAT#: SC126568

SLC22A4 (untagged)-Human solute carrier family 22 (organic cation/ergothioneine transporter), member 4 (SLC22A4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SLC22A4
• Synonyms: DFNB60; OCTN1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126568 sequence for NM_003059 edited (data generated by NextGen Sequencing)
  ATGCGGGACTACGACGAGGTGATCGCCTTCCTGGGCGAGTGGGGGCCCTTCCAGCGCCTC
  ATCTTCTTCCTGCTCAGCGCCAGCATCATCCCCAATGGCTTCAATGGTATGTCAGTCGTG
  TTCCTGGCGGGGACCCCGGAGCACCGCTGTCGAGTGCCGGACGCCGCGAACCTGAGCAGC
  GCCTGGCGCAACAACAGTGTCCCGCTGCGGCTGCGGGACGGCCGCGAGGTGCCCCACAGC
  TGCAGCCGCTACCGGCTCGCCACCATCGCCAACTTCTCGGCGCTCGGGCTGGAGCCGGGG
  CGCGACGTGGACCTGGGGCAGCTGGAGCAGGAGAGCTGCCTGGATGGCTGGGAGTTCAGC
  CAGGACGTCTACCTGTCCACCGTCGTGACCGAGTGGAATCTGGTGTGTGAGGACAACTGG
  AAGGTGCCCCTCACCACCTCCCTGTTCTTCGTAGGCGTGCTCCTCGGCTCCTTCGTGTCC
  GGGCAGCTGTCAGACAGGTTTGGCAGGAAGAACGTTCTCTTCGCAACCATGGCTGTACAG
  ACTGGCTTCAGCTTCCTGCAGATTTTCTCCATCAGCTGGGAGATGTTCACTGTGTTATTT
  GTCATCGTGGGCATGGGCCAGATCTCCAACTATGTGGTAGCCTTCATACTAGGAACAGAA
  ATTCTTGGCAAGTCAGTTCGTATTATATTCTCTACATTAGGAGTGTGCACATTTTTTGCA
  GTTGGCTATATGCTGCTGCCACTGTTTGCTTACTTCATCAGAGACTGGCGGATGCTGCTG
  CTGGCGCTGACGGTGCCGGGAGTGCTGTGTGTCCCGCTGTGGTGGTTCATTCCTGAATCT
  CCCCGATGGCTGATATCCCAGAGAAGATTTAGAGAGGCTGAAGATATCATCCAAAAAGCT
  GCAAAAATGAACAACATAGCTGTACCAGCAGTGATATTTGATTCTGTGGAGGAGCTAAAT
  CCCCTGAAGCAGCAGAAAGCTTTCATTCTGGACCTGTTCAGGACTCGGAATATTGCCATA
  ATGACCATTATGTCTTTGCTGCTATGGATGCTGACCTCAGTGGGTTACTTTGCTCTGTCT
  CTGGATGCTCCTAATTTACATGGAGATGCCTACCTGAACTGTTTCCTCTCTGCCTTGATT
  GAAATTCCAGCTTACATTACAGCCTGGCTGCTATTGCGAACCCTGCCCAGGCGTTATATC
  ATAGCTGCAGTACTGTTCTGGGGAGGAGGTGTCCTTCTCTTCATTCAACTGGTACCTGTG
  GATTATTACTTCTTATCCATTGGTCTGGTCATGCTGGGAAAATTTGGGATCACCTCTGCT
  TTCTCCATGCTGTATGTCTTCACTGCTGAGCTCTACCCAACCCTGGTCAGGAACATGGCG
  GTGGGGGTCACATCCACGGCCTCCAGAGTGGGCAGCATCATTGCCCCCTACTTTGTTTAC
  CTCGGTGCTTACAACAGAATGCTGCCCTACATCGTCATGGGTAGTCTGACTGTCCTGATT
  GGAATCCTCACCCTTTTTTTCCCTGAAAGTTTGGGAATGACTCTTCCAGAAACCTTAGAG
  CAGATGCAGAAAGTGAAATGGTTCAGATCTGGGAAAAAAACAAGAGACTCAATGGAGACA
  GAAGAAAATCCCAAGGTTCTAATAACTGCATTCTGA
  
  Clone variation with respect to NM_003059.2
  1233 g=>c
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003059 unedited
  NNGGTCAGGACAGAATTGTATACGACTCATATAGGCGGACCGCGAATCCCGGGCTCCTGT
  TTCCCAGNAACGGTCCCCGGCTTCGCGCCCCAATTTCTAACAGCCTGCCTGTCCCCCGGG
  AACGTTCTAACATCCTTGGGGAGCGCCCCAGCTACAAGACACTGTCCTGAGAACGCTGTC
  ATCACCCGTAGTTGCAAGTTTCGGAGCGGCAGTGGGAAGCATGCGGGACTACGACGAGGT
  GATCGCCTTCCTGGGCGAGTGGGGGCCCTTCCAGCGCCTCATCTTCTTCCTGCTCAGCGC
  CAGCATCATCCCCAATGGCTTCAATGGTATGTCAGTCGTGTTCCTGGCGGGGACCCCGGA
  GCACCGCTGTCGAGTGCCGGACGCCGCGAACCTGAGCAGCGCCTGGCGCAACAACAGTGT
  CCCGCTGCGGCTGCGGGACGGCCGCGAGGTGCCCCACAGCTGCAGCCGCTACCGGCTCGC
  CACCATCGCCAACTTCTCGGCGCTCGGGCTGGAGCCGGGGCGCGACGTGGACCTGGGGCA
  GCTGGAGCAGGAGAGCTGCCTGGATGGCTGGGAGTTCAGCCAGGACGTCTACCTGTCCAC
  CGTCGTGACCGAGTGGAATCTGGTGTGTGAGGACAACTGGAAGGTGCCCCTCACCACCTC
  CCTGTTCTTCGTAGGCGTGCTCCTCGGCTCCTTCGTGTCCGGGCAGCTGTCAGACAGGTT
  TGGCAGGAAGAACGTTCTCTTCGCAACCATGGCTGTACAGACTGGCTTCAGCTTCCTGCA
  GATTTTCTCCATCAGCTGGGAGATGTTCACTGTGTTATTTGTCATCGTGGGCATGGGCCA
  GATCTCCNACTATGTGGTAGCCT
  
• Restriction Sites: Please inquire
• ACCN: NM_003059
• Insert Size: 2300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003059.2, NP_003050.2
• RefSeq Size: 2214 bp
• RefSeq ORF: 1656 bp
• Locus ID: 6583
• UniProt ID: Q9H015
• Cytogenetics: 5q31.1
• Domains: sugar_tr
• Protein Families: Transmembrane
• Gene Summary: Polyspecific organic cation transporters in the liver, kidney, intestine, and other organs are critical for elimination of many endogenous small organic cations as well as a wide array of drugs and environmental toxins. The encoded protein is an organic cation transporter and plasma integral membrane protein containing eleven putative transmembrane domains as well as a nucleotide-binding site motif. Transport by this protein is at least partially ATP-dependent. [provided by RefSeq, Jul 2008]