EIF5A2 (NM_020390) Human Tagged ORF Clone
$225.00
In Stock*
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
|---|---|
| Target Symbol | EIF5A2 |
| Synonyms | EIF-5A2; eIF5AII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Chromatograms |
Chromatograms
 Sequencher program is needed, download here |
| Restriction Sites |
SgfI-MluI Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_020390 |
| ORF Size | 459 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_020390.6 |
| RefSeq Size | 5537 bp |
| RefSeq ORF | 462 bp |
| Locus ID | 56648 |
| UniProt ID | Q9GZV4 |
| Cytogenetics | 3q26.2 |
| MW | 16.8 kDa |
| Summary | mRNA-binding protein involved in translation elongation. Has an important function at the level of mRNA turnover, probably acting downstream of decapping. Involved in actin dynamics and cell cycle progression, mRNA decay and probably in a pathway involved in stress response and maintenance of cell wall integrity. Functions as a regulator of apoptosis. Mediates effects of polyamines on neuronal process extension and survival. May play an important role in brain development and function, and in skeletal muscle stem cell differentiation (By similarity).UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_020390" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| RC206249L1 | Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC206249L2 | Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), mGFP tagged | 10 ug |
$525.00
|
|
| RC206249L3 | Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), Myc-DDK-tagged | 10 ug |
$525.00
|
|
| RC206249L4 | Lenti ORF clone of Human eukaryotic translation initiation factor 5A2 (EIF5A2), mGFP tagged | 10 ug |
$525.00
|
|
| RG206249 | EIF5A2 (tGFP-tagged) - Human eukaryotic translation initiation factor 5A2 (EIF5A2) | 10 ug |
$425.00
|
|
| SC126117 | EIF5A2 (untagged)-Human eukaryotic translation initiation factor 5A2 (EIF5A2) | 10 ug |
$150.00
|
|
Citations
Laminin signals initiate the reciprocal loop that informs breast-specific gene expression and homeostasis by activating NO, p53 and microRNAs
Furuta, Ren, Mao et al
Elife (2018) 7 DOI: 10.7554/eLife.26148
Furuta, Ren, Mao et al
Elife (2018) 7 DOI: 10.7554/eLife.26148
AI Summary: OriGene contributed to this study by providing the SCA1 (Cat#RC222862) and EIF5A2 (Cat#RC206249) cDNA clones, which were used to generate overexpression constructs for investigating their roles in breast epithelial architecture and gene expression.
Snippet: … For the SCA1- and EIF5A2-overexpression lentivirus construct, the cDNA clones were obtained from Origene (Rockville, MD, USA, Cat#RC222862 and Cat#RC206249, respectively) and cloned into PCDF1-MCS2-EF1-puro lentiviral vector at the BamHI/EcoR1 site using the Gibson assembly system and a DNA ass;... … For the SCA1- and EIF5A2-overexpression lentivirus construct, the cDNA clones were obtained from Origene (Rockville, MD, USA, Cat#RC222862 and Cat#RC206249, respectively) and cloned into PCDF1-MCS2-EF1-puro lentiviral vector at the BamHI/EcoR1 site using the Gibson assembly system and a DNA ass
Showing 1-1 of 1 papers.
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