PLK4 (NM_014264) Human Tagged ORF Clone

CAT#: RC206015

PLK4 (Myc-DDK-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 1

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY PLK4 (Cat# RC206015, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-PLK4 (Cat# TA810562)(1:2000). Positive lysates LY402302 (100ug) and LC402302 (20ug) can be purchased separately from OriGene.

• 

  Western blot validation of overexpression lysate (Cat# LY402302) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC206015 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: PLK4
• Synonyms: MCCRP2; SAK; STK18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC206015 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGGAAATCTGCTTGGTAAAGGATCATTTG
  CTGGTGTCTACAGAGCTGAGTCCATTCACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC
  CATGTACAAAGCAGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAATTGAAACATCCT
  TCTATCTTGGAGCTTTATAACTATTTTGAAGATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATA
  ATGGAGAAATGAACAGGTATCTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACACTTCAT
  GCACCAGATCATCACAGGGATGTTGTATCTTCATTCTCATGGTATACTACACCGGGACCTCACACTTTCT
  AACCTCCTACTGACTCGTAATATGAACATCAAGATTGCTGATTTTGGGCTGGCAACTCAACTGAAAATGC
  CACATGAAAAGCACTATACATTATGTGGAACTCCTAACTACATTTCACCAGAAATTGCCACTCGAAGTGC
  ACATGGCCTTGAATCTGATGTTTGGTCCCTGGGCTGTATGTTTTATACATTACTTATCGGGAGACCACCC
  TTCGACACTGACACAGTCAAGAACACATTAAATAAAGTAGTATTGGCAGATTATGAAATGCCATCTTTTT
  TGTCAATAGAGGCCAAGGACCTTATTCACCAGTTACTTCGTAGAAATCCAGCAGATCGTTTAAGTCTGTC
  TTCAGTATTGGACCATCCTTTTATGTCCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAA
  GACTCAATTGATAGTGGGCATGCCACAATTTCTACTGCAATTACAGCTTCTTCCAGTACCAGTATAAGTG
  GTAGTTTATTTGACAAAAGAAGACTTTTGATTGGTCAGCCACTCCCAAATAAAATGACTGTATTTCCAAA
  GAATAAAAGTTCAACTGATTTTTCTTCTTCAGGAGATGGAAACAGTTTTTATACTCAGTGGGGAAATCAA
  GAAACCAGTAATAGTGGAAGGGGAAGAGTAATTCAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTC
  GTAGAGCTTATTCCTCTGATAGATCTGGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGA
  ACGATGTCACTCAGCAGAAATGCTTTCAGTGTCCAAAAGATCAGGAGGAGGTGAAAATGAAGAGAGGTAC
  TCACCCACAGACAACAATGCCAACATTTTTAACTTCTTTAAAGAAAAGACATCCAGTAGTTCTGGATCTT
  TTGAAAGACCTGATAACAATCAAGCACTCTCCAATCATCTTTGTCCAGGAAAAACTCCTTTTCCATTTGC
  AGACCCGACACCTCAGACTGAAACCGTACAACAGTGGTTTGGGAATCTGCAAATAAATGCTCATTTAAGA
  AAAACTACTGAATATGACAGCATCAGCCCAAACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACA
  CATCAAAAAATGCCTGGACTGATACAAAAGTCAAAAAGAACTCTGATGCTTCTGATAATGCACATTCTGT
  AAAACAGCAAAATACCATGAAATATATGACTGCACTTCACAGTAAACCTGAGATAATCCAACAAGAATGT
  GTTTTTGGCTCAGATCCTCTTTCTGAACAGAGCAAGACTAGGGGTATGGAGCCACCATGGGGTTATCAGA
  ATCGTACATTAAGAAGCATTACATCTCCGTTGGTTGCTCACAGGTTAAAACCAATCAGACAGAAAACCAA
  AAAGGCTGTGGTGAGCATACTTGATTCAGAGGAGGTGTGTGTGGAGCTTGTAAAGGAGTATGCATCTCAA
  GAATATGTGAAAGAAGTTCTTCAGATATCTAGTGATGGAAATACGATCACTATTTATTATCCAAATGGTG
  GTAGAGGTTTTCCTCTTGCTGATAGACCACCCTCACCTACTGACAACATCAGTAGGTACAGCTTTGACAA
  TTTACCAGAAAAATACTGGCGAAAATATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCTAAATCT
  CCCAAAATCACTTATTTTACAAGATATGCTAAATGCATTTTGATGGAGAATTCTCCTGGTGCTGATTTTG
  AGGTTTGGTTTTATGATGGGGTAAAAATACACAAAACAGAAGATTTCATTCAGGTGATTGAAAAGACAGG
  GAAGTCTTACACTTTAAAAAGTGAAAGTGAAGTTAATAGCTTGAAAGAGGAGATAAAAATGTATATGGAC
  CATGCTAATGAGGGTCATCGTATTTGTTTAGCACTGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTA
  GGAGTGCTCCCTTTTTCCCAATAATCATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGCCTTATC
  ACCTCCTCCTTCTGTGGATTCAAATTACCCAACGAGAGAGAGAGCATCTTTCAACAGAATGGTCATGCAT
  AGTGCTGCTTCTCCAACACAGGCACCAATCCTTAATCCCTCTATGGTTACAAATGAAGGACTTGGTCTTA
  CAACTACAGCTTCTGGAACAGACATCTCTTCTAATAGTCTAAAAGATTGTCTTCCTAAATCAGCACAACT
  TTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGTTAACTAGTGGAGCTGTGTGGGTTCAG
  TTTAATGATGGGTCCCAGTTGGTTGTGCAGGCAGGAGTGTCTTCTATCAGTTATACCTCACCAAATGGTC
  AAACAACTAGGTATGGAGAAAATGAAAAATTACCAGACTACATCAAACAGAAATTACAGTGTCTGTCTTC
  CATCCTTTTGATGTTTTCTAATCCGACTCCTAATTTTCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC206015 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHP
  SILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLS
  NLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPP
  FDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVE
  DSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQ
  ETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERY
  SPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLR
  KTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQEC
  VFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQ
  EYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKS
  PKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMD
  HANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMH
  SAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQ
  FNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014264
• ORF Size: 2910 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014264.5
• RefSeq Size: 3870 bp
• RefSeq ORF: 2913 bp
• Locus ID: 10733
• UniProt ID: O00444
• Cytogenetics: 4q28.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• MW: 109 kDa
• Gene Summary: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]