PLK4 (NM_014264) Human Tagged ORF Clone

CAT#: RC206015

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PLK4 (Myc-DDK-tagged)-Human polo-like kinase 4 (PLK4), transcript variant 1



  "NM_014264" in other vectors (7)

Reconstitution Protocol

USD 1,332.00


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Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol PLK4
Synonyms MCCRP2; SAK; STK18
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC206015 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGGAAATCTGCTTGGTAAAGGATCATTTG
CTGGTGTCTACAGAGCTGAGTCCATTCACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC
CATGTACAAAGCAGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAATTGAAACATCCT
TCTATCTTGGAGCTTTATAACTATTTTGAAGATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATA
ATGGAGAAATGAACAGGTATCTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACACTTCAT
GCACCAGATCATCACAGGGATGTTGTATCTTCATTCTCATGGTATACTACACCGGGACCTCACACTTTCT
AACCTCCTACTGACTCGTAATATGAACATCAAGATTGCTGATTTTGGGCTGGCAACTCAACTGAAAATGC
CACATGAAAAGCACTATACATTATGTGGAACTCCTAACTACATTTCACCAGAAATTGCCACTCGAAGTGC
ACATGGCCTTGAATCTGATGTTTGGTCCCTGGGCTGTATGTTTTATACATTACTTATCGGGAGACCACCC
TTCGACACTGACACAGTCAAGAACACATTAAATAAAGTAGTATTGGCAGATTATGAAATGCCATCTTTTT
TGTCAATAGAGGCCAAGGACCTTATTCACCAGTTACTTCGTAGAAATCCAGCAGATCGTTTAAGTCTGTC
TTCAGTATTGGACCATCCTTTTATGTCCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAA
GACTCAATTGATAGTGGGCATGCCACAATTTCTACTGCAATTACAGCTTCTTCCAGTACCAGTATAAGTG
GTAGTTTATTTGACAAAAGAAGACTTTTGATTGGTCAGCCACTCCCAAATAAAATGACTGTATTTCCAAA
GAATAAAAGTTCAACTGATTTTTCTTCTTCAGGAGATGGAAACAGTTTTTATACTCAGTGGGGAAATCAA
GAAACCAGTAATAGTGGAAGGGGAAGAGTAATTCAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTC
GTAGAGCTTATTCCTCTGATAGATCTGGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGA
ACGATGTCACTCAGCAGAAATGCTTTCAGTGTCCAAAAGATCAGGAGGAGGTGAAAATGAAGAGAGGTAC
TCACCCACAGACAACAATGCCAACATTTTTAACTTCTTTAAAGAAAAGACATCCAGTAGTTCTGGATCTT
TTGAAAGACCTGATAACAATCAAGCACTCTCCAATCATCTTTGTCCAGGAAAAACTCCTTTTCCATTTGC
AGACCCGACACCTCAGACTGAAACCGTACAACAGTGGTTTGGGAATCTGCAAATAAATGCTCATTTAAGA
AAAACTACTGAATATGACAGCATCAGCCCAAACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACA
CATCAAAAAATGCCTGGACTGATACAAAAGTCAAAAAGAACTCTGATGCTTCTGATAATGCACATTCTGT
AAAACAGCAAAATACCATGAAATATATGACTGCACTTCACAGTAAACCTGAGATAATCCAACAAGAATGT
GTTTTTGGCTCAGATCCTCTTTCTGAACAGAGCAAGACTAGGGGTATGGAGCCACCATGGGGTTATCAGA
ATCGTACATTAAGAAGCATTACATCTCCGTTGGTTGCTCACAGGTTAAAACCAATCAGACAGAAAACCAA
AAAGGCTGTGGTGAGCATACTTGATTCAGAGGAGGTGTGTGTGGAGCTTGTAAAGGAGTATGCATCTCAA
GAATATGTGAAAGAAGTTCTTCAGATATCTAGTGATGGAAATACGATCACTATTTATTATCCAAATGGTG
GTAGAGGTTTTCCTCTTGCTGATAGACCACCCTCACCTACTGACAACATCAGTAGGTACAGCTTTGACAA
TTTACCAGAAAAATACTGGCGAAAATATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCTAAATCT
CCCAAAATCACTTATTTTACAAGATATGCTAAATGCATTTTGATGGAGAATTCTCCTGGTGCTGATTTTG
AGGTTTGGTTTTATGATGGGGTAAAAATACACAAAACAGAAGATTTCATTCAGGTGATTGAAAAGACAGG
GAAGTCTTACACTTTAAAAAGTGAAAGTGAAGTTAATAGCTTGAAAGAGGAGATAAAAATGTATATGGAC
CATGCTAATGAGGGTCATCGTATTTGTTTAGCACTGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTA
GGAGTGCTCCCTTTTTCCCAATAATCATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGCCTTATC
ACCTCCTCCTTCTGTGGATTCAAATTACCCAACGAGAGAGAGAGCATCTTTCAACAGAATGGTCATGCAT
AGTGCTGCTTCTCCAACACAGGCACCAATCCTTAATCCCTCTATGGTTACAAATGAAGGACTTGGTCTTA
CAACTACAGCTTCTGGAACAGACATCTCTTCTAATAGTCTAAAAGATTGTCTTCCTAAATCAGCACAACT
TTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGTTAACTAGTGGAGCTGTGTGGGTTCAG
TTTAATGATGGGTCCCAGTTGGTTGTGCAGGCAGGAGTGTCTTCTATCAGTTATACCTCACCAAATGGTC
AAACAACTAGGTATGGAGAAAATGAAAAATTACCAGACTACATCAAACAGAAATTACAGTGTCTGTCTTC
CATCCTTTTGATGTTTTCTAATCCGACTCCTAATTTTCAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC206015 protein sequence
Red=Cloning site Green=Tags(s)

MATCIGEKIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHP
SILELYNYFEDSNYVYLVLEMCHNGEMNRYLKNRVKPFSENEARHFMHQIITGMLYLHSHGILHRDLTLS
NLLLTRNMNIKIADFGLATQLKMPHEKHYTLCGTPNYISPEIATRSAHGLESDVWSLGCMFYTLLIGRPP
FDTDTVKNTLNKVVLADYEMPSFLSIEAKDLIHQLLRRNPADRLSLSSVLDHPFMSRNSSTKSKDLGTVE
DSIDSGHATISTAITASSSTSISGSLFDKRRLLIGQPLPNKMTVFPKNKSSTDFSSSGDGNSFYTQWGNQ
ETSNSGRGRVIQDAEERPHSRYLRRAYSSDRSGTSNSQSQAKTYTMERCHSAEMLSVSKRSGGGENEERY
SPTDNNANIFNFFKEKTSSSSGSFERPDNNQALSNHLCPGKTPFPFADPTPQTETVQQWFGNLQINAHLR
KTTEYDSISPNRDFQGHPDLQKDTSKNAWTDTKVKKNSDASDNAHSVKQQNTMKYMTALHSKPEIIQQEC
VFGSDPLSEQSKTRGMEPPWGYQNRTLRSITSPLVAHRLKPIRQKTKKAVVSILDSEEVCVELVKEYASQ
EYVKEVLQISSDGNTITIYYPNGGRGFPLADRPPSPTDNISRYSFDNLPEKYWRKYQYASRFVQLVRSKS
PKITYFTRYAKCILMENSPGADFEVWFYDGVKIHKTEDFIQVIEKTGKSYTLKSESEVNSLKEEIKMYMD
HANEGHRICLALESIISEEERKTRSAPFFPIIIGRKPGSTSSPKALSPPPSVDSNYPTRERASFNRMVMH
SAASPTQAPILNPSMVTNEGLGLTTTASGTDISSNSLKDCLPKSAQLLKSVFVKNVGWATQLTSGAVWVQ
FNDGSQLVVQAGVSSISYTSPNGQTTRYGENEKLPDYIKQKLQCLSSILLMFSNPTPNFH

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_014264
ORF Size 2910 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_014264.5
RefSeq Size 3870 bp
RefSeq ORF 2913 bp
Locus ID 10733
UniProt ID O00444
Cytogenetics 4q28.1
Domains pkinase, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
MW 109 kDa
Gene Summary This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]

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