PLK4 (NM_014264) Human Untagged Clone

CAT#: SC115082

PLK4 (untagged)-Human polo-like kinase 4 (PLK4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLK4
• Synonyms: MCCRP2; SAK; STK18
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_014264 edited
  ATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGGAAATCTGCTTGGTAAA
  GGATCATTTGCTGGTGTCTACAGAGCTGAGTCCATTCACACTGGTTTGGAAGTTGCAATC
  AAAATGATAGATAAGAAAGCCATGTACAAAGCAGGAATGGTACAGAGAGTCCAAAATGAG
  GTGAAAATACATTGCCAATTGAAACATCCTTCTATCTTGGAGCTTTATAACTATTTTGAA
  GATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATAATGGAGAAATGAACAGGTAT
  CTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACACTTCATGCACCAGATC
  ATCACAGGGATGTTGTATCTTCATTCTCATGGTATACTACACCGGGACCTCACACTTTCT
  AACCTCCTACTGACTCGTAATATGAACATCAAGATTGCTGATTTTGGGCTGGCAACTCAA
  CTGAAAATGCCACATGAAAAGCACTATACATTATGTGGAACTCCTAACTACATTTCACCA
  GAAATTGCCACTCGAAGTGCACATGGCCTTGAATCTGATGTTTGGTCCCTGGGCTGTATG
  TTTTATACATTACTTATCGGGAGACCACCCTTCGACACTGACACAGTCAAGAACACATTA
  AATAAAGTAGTATTGGCAGATTATGAAATGCCATCTTTTTTGTCAATAGAGGCCAAGGAC
  CTTATTCACCAGTTACTTCGTAGAAATCCAGCAGATCGTTTAAGTCTGTCTTCAGTATTG
  GACCATCCTTTTATGTCCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAA
  GACTCAATTGATAGTGGGCATGCCACAATTTCTACTGCAATTACAGCTTCTTCCAGTACC
  AGTATAAGTGGTAGTTTATTTGACAAAAGAAGACTTTTGATTGGTCAGCCACTCCCAAAT
  AAAATGACTGTATTTCCAAAGAATAAAAGTTCAACTGATTTTTCTTCTTCAGGAGATGGA
  AACAGTTTTTATACTCAGTGGGGAAATCAAGAAACCAGTAATAGTGGAAGGGGAAGAGTA
  ATTCAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTCGTAGAGCTTATTCCTCTGAT
  AGATCTGGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGAACGATGTCAC
  TCAGCAGAAATGCTTTCAGTGTCCAAAAGATCAGGAGGAGGTGAAAATGAAGAGAGGTAC
  TCACCCACAGACAACAATGCCAACATTTTTAACTTCTTTAAAGAAAAGACATCCAGTAGT
  TCTGGATCTTTTGAAAGACCTGATAACAATCAAGCACTCTCCAATCATCTTTGTCCAGGA
  AAAACTCCTTTTCCATTTGCAGACCCGACACCTCAGACTGAAACCGTACAACAGTGGTTT
  GGGAATCTGCAAATAAATGCTCATTTAAGAAAAACTACTGAATATGACAGCATCAGCCCA
  AACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACACATCAAAAAATGCCTGGACT
  GATACAAAAGTCAAAAAGAACTCTGATGCTTCTGATAATGCACATTCTGTAAAACAGCAA
  AATACCATGAAATATATGACTGCACTTCACAGTAAACCTGAGATAATCCAACAAGAATGT
  GTTTTTGGCTCAGATCCTCTTTCTGAACAGAGCAAGACTAGGGGTATGGAGCCACCATGG
  GGTTATCAGAATCGTACATTAAGAAGCATTACATCTCCGTTGGTTGCTCACAGGTTAAAA
  CCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATACTTGATTCAGAGGAGGTGTGT
  GTGGAGCTTGTAAAGGAGTATGCATCTCAAGAATATGTGAAAGAAGTTCTTCAGATATCT
  AGTGATGGAAATACGATCACTATTTATTATCCAAATGGTGGTAGAGGTTTTCCTCTTGCT
  GATAGACCACCCTCACCTACTGACAACATCAGTAGGTACAGCTTTGACAATTTACCAGAA
  AAATACTGGCGAAAATATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCTAAATCT
  CCCAAAATCACTTATTTTACAAGATATGCTAAATGCATTTTGATGGAGAATTCTCCTGGT
  GCTGATTTTGAGGTTTGGTTTTATGATGGGGTAAAAATACACAAAACAGAAGATTTCATT
  CAGGTGATTGAAAAGACAGGGAAGTCTTACACTTTAAAAAGTGAAAGTGAAGTTAATAGC
  TTGAAAGAGGAGATAAAAATGTATATGGACCATGCTAATGAGGGTCATCGTATTTGTTTA
  GCACTGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTAGGAGTGCTCCCTTTTTCCCA
  ATAATCATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGCCTTATCACCTCCTCCT
  TCTGTGGATTCAAATTACCCAACGAGAGAGAGAGCATCTTTCAACAGAATGGTCATGCAT
  AGTGCTGCTTCTCCAACACAGGCACCAATCCTTAATCCCTCTATGGTTACAAATGAAGGA
  CTTGGTCTTACAACTACAGCTTCTGGAACAGACATCTCTTCTAATAGTCTAAAAGATTGT
  CTTCCTAAATCAGCACAACTTTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACA
  CAGTTAACTAGTGGAGCTGTGTGGGTTCAGTTTAATGATGGGTCCCAGTTGGTTGTGCAG
  GCAGGAGTGTCTTCTATCAGTTATACCTCACCAAATGGTCAAACAACTAGGTATGGAGAA
  AATGAAAAATTACCAGACTACATCAAACAGAAATTACAGTGTCTGTCTTCCATCCTTTTG
  ATGTTTTCTAATCCGACTCCTAATTTTCATTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014264 unedited
  TTTTTGTATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGNAGATTTTCAAAAT
  GGGAGCCCAGAGGCACCGCCCAGGCCTCGGAAGGTGTCAGGGAGAACTTTCCGTGGTTTC
  AGCGTCGTCGCCTGGAGCGGCGGTTTAGAGAGCCGAGCCTGATGGGCGCCAAGGCCGGCT
  GGCTGCTTGGAGCGCTGCCTCGAAGGGACTGCGTGAAGGAAGCTAATCCGGAGAACCCAG
  GCCAGAGCCTGGAAATATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGG
  AAATCTGCTTGGTAAAGGATCATTTGCTGGTGTCTACAGAGCTGAGTCCATTCACACTGG
  TTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGCCATGTACAAAGCAGGAATGGTACA
  GAGAGTCCAAAATGAGGTGAAAATACATTGCCAATTGAAACATCCTTCTATCTTGGAGCT
  TTATAACTATTTTGAAGATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATAATGG
  AGAAATGAACAGGTATCTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACA
  CTTCATGCACCAGATCATCACAGGGATGTTGTATCTTCATTCTCATGGTATACTACACCG
  GGACCTCACACTTTCTAACCTCCTACTGACTCGTAATATGAACATCAAGATTGCTGATTT
  TGGGCTNGGCACTCAACTGAAAATGCCACATGANAAGCACTATACATTATGTGGAACTCC
  TAACTACATTTCACCAGAAATTGCCACTCGAAGTGCACATGGCCTTNNGATCTGATGTTG
  GTCCCTGGGCTGTATGNTNTATACATTACTTATCGGGAGAACCACCTTCGCACTNGACAC
  AGTCAGAACACATAAATANAGTAGTATGGGCAGTTNATGAATGCCATCTTTTNTGTCATA
  GAGGCCAGGNACTTATTCACAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_014264 unedited
  CTTGGCCGCGGCCGCAATCTAGAGNCGAGNTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTGGAACTTAACACTATTTTTATTTTTTTATAAAAAGCATTTGCAAAAATGGT
  TCATCAAACACTGGGGTTACCAAAATAAATATAAAACAAATATACCTGGTTACATATGGT
  ACTTATAACATAAATACCCATTATTTACATTTTCAAAAATTCCAAAATTCCAGCAGGTTT
  TGGCCATGATTCTCAACATGTCTGAAACCCCGGGAAAACAATAAAAGCCCAAATAAACTA
  CCCATCATTTACATTTTTGCTTTTAAAAAAATGGTCAATACAAATATATGAACATAGTAA
  GTTCGGGGGTTATGGGCTAAAAACAAAGTCAGGGGGTCTATTTTATATCCACTATATTTA
  ATAAGGGACTCAAGGTTCATTCTGCTTTGGTCTCTCAAAATACATCCTTTACATTATAGG
  GTAAAATTCTTTCATAAAAAGGGTTTCTGAAAACTTTATGTTAAATTAAAAAAAATCACT
  TTACTTGAAAGGCAACAAAAAAGGTATTTATTAAACTTATATGTCTGAAAGGAGTTTTAA
  TCAATGAAAATTAGGAGTCGGATTAAAAAACATCAAAAGGGTGGAAGACAGACACTGGAA
  TTTCTGTTTGAAGGAACCTGGGAATTTTTCATTTTCTCCATACCCTAATTGGTTGACCAT
  TTGGNGAGGGATAACTGATAGAAGACACTTCTGGCTGCACAACCAACTGGGACCCATCAT
  TAAACTGGACCCACCAGCTTCCCTAGTTAACTGTGTAGCCCAACCACATTTTTCACAAAA
  CACGATTCAAAAGTTGGGCTGGATTAAGAAGACATTCTTTAGACATTAAAAGAGAGTCTG
  TCCCAAAGCTGATTTGTAAAACCAAGTCTCCATTGAAACATAAAGGATAAAGATAGGGCC
  TGGTTGAGAAACAACT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014264
• Insert Size: 3970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014264.2, NP_055079.2
• RefSeq Size: 3331 bp
• RefSeq ORF: 2913 bp
• Locus ID: 10733
• UniProt ID: O00444
• Cytogenetics: 4q28.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).