PLK4 (NM_014264) Human Untagged Clone

CAT#: SC323369

PLK4 (untagged)-Kinase deficient mutant (K41M) of Human polo-like kinase 4 (Drosophila) (PLK4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLK4
• Synonyms: MCCRP2; SAK; STK18
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC323369 sequence for NM_014264 edited (data generated by NextGen Sequencing)
  ATGGCGACCTGCATCGGGGAGAAGATCGAGGATTTTAAAGTTGGAAATCTGCTTGGTAAA
  GGATCATTTGCTGGTGTCTACAGAGCTGAGTCCATTCACACTGGTTTGGAAGTTGCAATC
  ANNATGATAGATAAGAAAGCCATGTACAAAGCAGGAATGGTACAGAGAGTCCAAAATGAG
  GTGAAAATACATTGCCAATTGAAACATCCTTCTATCTTGGAGCTTTATAACTATTTTGAA
  GATAGCAATTATGTGTATCTGGTATTAGAAATGTGCCATAATGGAGAAATGAACAGGTAT
  CTAAAGAATAGAGTGAAACCCTTCTCAGAAAATGAAGCTCGACACTTCATGCACCAGATC
  ATCACAGGGATGTTGTATCTTCATTCTCATGGTATACTACACCGGGACCTCACACTTTCT
  AACCTCCTACTGACTCGTAATATGAACATCAAGATTGCTGATTTTGGGCTGGCAACTCAA
  CTGAAAATGCCACATGAAAAGCACTATACATTATGTGGAACTCCTAACTACATTTCACCA
  GAAATTGCCACTCGAAGTGCACATGGCCTTGAATCTGATGTTTGGTCCCTGGGCTGTATG
  TTTTATACATTACTTATCGGGAGACCACCCTTCGACACTGACACAGTCAAGAACACATTA
  AATAAAGTAGTATTGGCAGATTATGAAATGCCATCTTTTTTGTCAATAGAGGCCAAGGAC
  CTTATTCACCAGTTACTTCGTAGAAATCCAGCAGATCGTTTAAGTCTGTCTTCAGTATTG
  GACCATCCTTTTATGTCCCGAAATTCTTCAACAAAAAGTAAAGATTTAGGAACTGTGGAA
  GACTCAATTGATAGTGGGCATGCCACAATTTCTACTGCAATTACAGCTTCTTCCAGTACC
  AGTATAAGTGGTAGTTTATTTGACAAAAGAAGACTTTTGATTGGTCAGCCACTCCCAAAT
  AAAATGACTGTATTTCCAAAGAATAAAAGTTCAACTGATTTTTCTTCTTCAGGAGATGGA
  AACAGTTTTTATACTCAGTGGGGAAATCAAGAAACCAGTAATAGTGGAAGGGGAAGAGTA
  ATTCAAGATGCAGAAGAAAGGCCACATTCTCGATACCTTCGTAGAGCTTATTCCTCTGAT
  AGATCTGGCACTTCTAATAGTCAGTCTCAAGCAAAAACATATACAATGGAACGATGTCAC
  TCAGCAGAAATGCTTTCAGTGTCCAAAAGATCAGGAGGAGGTGAAAATGAAGAGAGGTAC
  TCACCCACAGACAACAATGCCAACATTTTTAACTTCTTTAAAGAAAAGACATCCAGTAGT
  TCTGGATCTTTTGAAAGACCTGATAACAATCAAGCACTCTCCAATCATCTTTGTCCAGGA
  AAAACTCCTTTTCCATTTGCAGACCCGACACCTCAGACTGAAACCGTACAACAGTGGTTT
  GGGAATCTGCAAATAAATGCTCATTTAAGAAAAACTACTGAATATGACAGCATCAGCCCA
  AACCGGGACTTCCAGGGCCATCCAGATTTGCAGAAGGACACATCAAAAAATGCCTGGACT
  GATACAAAAGTCAAAAAGAACTCTGATGCTTCTGATAATGCACATTCTGTAAAACAGCAA
  AATACCATGAAATATATGACTGCACTTCACAGTAAACCTGAGATAATCCAACAAGAATGT
  GTTTTTGGCTCAGATCCTCTTTCTGAACAGAGCAAGACTAGGGGTATGGAGCCACCATGG
  GGTTATCAGAATCGTACATTAAGAAGCATTACATCTCCGTTGGTTGCTCACAGGTTAAAA
  CCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATACTTGATTCAGAGGAGGTGTGT
  GTGGAGCTTGTAAAGGAGTATGCATCTCAAGAATATGTGAAAGAAGTTCTTCAGATATCT
  AGTGATGGAAATACGATCACTATTTATTATCCAAATGGTGGTAGAGGTTTTCCTCTTGCT
  GATAGACCACCCTCACCTACTGACAACATCAGTAGGTACAGCTTTGACAATTTACCAGAA
  AAATACTGGCGAAAATATCAATATGCTTCCAGGTTTGTACAGCTTGTAAGATCTAAATCT
  CCCAAAATCACTTATTTTACAAGATATGCTAAATGCATTTTGATGGAGAATTCTCCTGGT
  GCTGATTTTGAGGTTTGGTTTTATGATGGGGTAAAAATACACAAAACAGAAGATTTCATT
  CAGGTGATTGAAAAGACAGGGAAGTCTTACACTTTAAAAAGTGAAAGTGAAGTTAATAGC
  TTGAAAGAGGAGATAAAAATGTATATGGACCATGCTAATGAGGGTCATCGTATTTGTTTA
  GCACTGGAATCCATAATTTCAGAAGAGGAAAGGAAAACTAGGAGTGCTCCCTTTTTCCCA
  ATAATCATAGGAAGAAAACCTGGTAGTACTAGTTCACCTAAGGCCTTATCACCTCCTCCT
  TCTGTGGATTCAAATTACCCAACGAGAGAGAGAGCATCTTTCAACAGAATGGTCATGCAT
  AGTGCTGCTTCTCCAACACAGGCACCAATCCTTAATCCCTCTATGGTTACAAATGAAGGA
  CTTGGTCTTACAACTACAGCTTCTGGAACAGACATCTCTTCTAATAGTCTAAAAGATTGT
  CTTCCTAAATCAGCACAACTTTTGAAATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACA
  CAGTTAACTAGTGGAGCTGTGTGGGTTCAGTTTAATGATGGGTCCCAGTTGGTTGTGCAG
  GCAGGAGTGTCTTCTATCAGTTATACCTCACCAAATGGTCAAACAACTAGGTATGGAGAA
  AATGAAAAATTACCAGACTACATCAAACAGAAATTACAGTGTCTGTCTTCCATCCTTTTG
  ATGTTTTCTAATCCGACTCCTAATTTTCATTGA
  
  Clone variation with respect to NM_014264.4
  122 a=>n;123 a=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for mutant NM_014264 unedited
  CCGCCGTTGAGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAAC
  CGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGATTTTCAAAATGG
  GAGCCCAGAGGCACCGCCCAGGCCTCGGAAGGTGTCAGGGAGAACTTTCCGTGGTTTCAGCGTCGTCGCC
  TGGAGCGGCGGTTTAGAGAGCCGAGCCTGATGGGCGCCAAGGCCGGCTGGCTGCTTGGAGCACTGCCTCG
  AAGGGACTGCGTGAAGGAAGCTAATCCGGAGAAACCCAGGCCAGAGCCCTGGAAATATGGGGGACCTGCA
  TCGGGGAGAAAGATCGAGGATTTTAAAGTTGAAATCTGCTTGGGTAAAGGATCATTTGCTGGTGGTCTAC
  GGAGCTGAGTCCCATTAGACGGGTTGGGAGGTGCAATCATGATGGATGGATAAGAAGGCATGGAAAAAGC
  AGGGATTGTAAGAGAGAGTCAAAAAGGAGGTGAAAATCATGGCCATTTGAAATTCCTCTATCTTTGGACC
  TTTAACATTTTTGAAAATGGCATATGTGTGACTGGGTTTAGAAATGGCCAAGGGAAAGGACCGGTTCTAG
  AATAGGGACCCTTCCAAATGAACCGCCTTACGCCCATATCCCGGAGTGTGATCATTCTAGGGTTACACCG
  GACCTCACATTAACCCATAGCTGAATGACACACAATGGATTGTCGCACTCTGAATGCCTGAGAGCAACTA
  TTGAAACTGACTTCCGAATGCCATGACTGCTCACGGTGGGCCGCTGCTAACATCGGAACCTGATAGAAAA
  AAAA
  
  Kinase domain sequence

  >SC323369 kinase domain raw sequence. By performing BLASTX analysis with this sequence against NCBI refernce protein database, you can confirm the presence of the kinase-deficient mutation
  CSACKGMGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAGCAGAGCTCGTTTAGTGAACCGT
  CAGAATTTTGTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGAGATTTTCAAAATGGGAG
  CCCAGAGGCACCGCCCAGGCCTCGGAAGGTGTCAGGGAGAACTTTCCGTGGTTTCAGCGTCGTCGCCTGG
  AGCGGCGGTTTAGAGAGCCGAGCCTGATGGGCGCCAAGGCCGGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_014264
• Insert Size: 3970 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This kinase-deficient mutant clone was generated by created by site-directed mutagenesis from the corresponding wild-type clone. See details in "Application of active and kinase-deficient kinome collection for identification of kinases regulating hedgehog signaling." Cell. 2008 May p536-548.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014264.2, NP_055079.2
• RefSeq Size: 3331 bp
• RefSeq ORF: 2913 bp
• Locus ID: 10733
• UniProt ID: O00444
• Cytogenetics: 4q28.1
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to centrioles, complex microtubule-based structures found in centrosomes, and regulates centriole duplication during the cell cycle. Three alternatively spliced transcript variants that encode different protein isoforms have been found for this gene. [provided by RefSeq, Jun 2010]
  Transcript Variant: This variant (1) represents the longest transcript and it encodes the longest protein (isoform 1).