FGD1 (NM_004463) Human Tagged ORF Clone

SKU RC205133

FGD1 (Myc-DDK-tagged)-Human FYVE, RhoGEF and PH domain containing 1 (FGD1)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: FGD1
• Synonyms: AAS; FGDY; MRXS16; ZFYVE3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC205133 representing NM_004463; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGCATGGCCACCGAGCCCCGGGGGGCGCCGGGCCTTCGGAGCCCGAACACCCGGCCACGAACCCGCCGG; GCGCCGCTCCGCCGGCCTGTGCCGACTCGGACCCTGGAGCCTCGGAACCCGGACTGCTGGCGCGCAGGGG; CTCAGGTTCGGCTCTTGGCGGCCCACTGGATCCCCAGTTTGTCGGACCCTCGGACACCAGCCTGGGCGCT; GCTCCAGGCCACCGGGTCTTGCCCTGCGGTCCCAGTCCACAGCACCACCGGGCCCTGCGCTTCTCTTACC; ACCTGGAGGGCTCGCAGCCTCGGCCTGGGCTGCACCAGGGAAACCGGATCCTGGTTAAAAGTTTGTCCCT; TGACCCTGGCCAAAGCCTAGAGCCTCATCCAGAAGGTCCCCAGCGGCTTCGCTCAGACCCAGGTCCCCCG; ACTGAAACCCCTAGCCAGCGTCCTTCACCACTGAAGCGGGCACCGGGCCCGAAGCCACAGGTGCCCCCAA; AGCCCAGCTACCTGCAGATGCCCCGGATGCCCCCCCCACTGGAGCCCATCCCCCCTCCACCATCACGCCC; ACTGCCTGCCGACCCCCGAGTGGCCAAGGGCCTGGCTCCCAGGGCAGAGGCCAGCCCCAGTTCTGCAGCA; GTATCCTCACTGATTGAGAAGTTTGAAAGAGAGCCTGTGATTGTCGCCTCGGATAGACCAGTCCCTGGCC; CCAGCCCAGGTCCCCCAGAGCCAGTCATGTTGCCACAGCCAACCTCGCAGCCACCAGTGCCCCAGCTCCC; CGAGGGTGAGGCCTCCCGCTGCCTGTTTCTGCTGGCTCCTGGGCCCCGGGACGGTGAGAAGGTGCCCAAC; CGGGACAGCGGCATTGATAGCATCAGCTCGCCATCCAACAGCGAGGAGACCTGCTTCGTCAGTGATGACG; GGCCCCCCAGCCACAGCCTCTGCCCTGGGCCCCCTGCCCTGGCTAGTGTGCCTGTTGCCTTGGCCGACCC; CCACCGGCCTGGCTCCCAAGAGGTTGACAGTGACCTGGAGGAGGAGGACGACGAGGAGGAGGAGGAAGAG; AAGGACAGAGAAATCCCAGTGCCCCTGATGGAGAGACAGGAGTCTGTGGAGTTGACTGTGCAGCAAAAGG; TGTTTCACATTGCCAATGAGCTCCTGCAAACTGAGAAGGCCTACGTTTCCAGGCTCCATCTCCTGGATCA; GGTGTTCTGTGCCCGGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCCGGCCGACGTTGTCCACGGC; ATCTTCTCTAACATCTGCTCCATCTATTGCTTCCACCAGCAGTTCCTGCTGCCTGAGCTAGAGAAGCGCA; TGGAGGAATGGGACCGCTATCCACGCATTGGAGACATCCTGCAGAAACTGGCCCCCTTCCTCAAGATGTA; TGGTGAGTATGTGAAGAACTTTGACCGGGCCGTGGAGCTGGTCAACACCTGGACAGAGCGCTCCACCCAG; TTTAAAGTCATCATCCATGAGGTGCAGAAGGAGGAAGCCTGTGGCAACCTGACATTGCAGCACCACATGC; TGGAGCCTGTGCAGCGCATCCCCCGCTATGAGCTTCTTCTCAAGGACTATCTGTTAAAGCTGCCCCATGG; CTCCCCGGACAGCAAGGATGCCCAAAAGTCTCTGGAGCTGATCGCCACAGCAGCAGAGCACTCGAATGCT; GCCATCCGCAAAATGGAGCGAATGCATAAGCTGCTGAAGGTATATGAGCTGTTAGGGGGCGAGGAGGACA; TTGTCAGCCCCACCAAAGAGCTCATAAAAGAAGGCCACATCCTTAAGCTGTCAGCAAAGAATGGGACCAC; TCAAGACCGATACCTCATACTATTCAACGACCGCCTCCTTTACTGCGTGCCCAGGCTGCGGCTCCTTGGC; CAGAAGTTTAGCGTGCGGGCACGCATTGATGTAGATGGCATGGAGCTAAAGGAGAGCTCCAACCTCAATC; TGCCTCGAACCTTCCTGGTGTCAGGAAAGCAGCGCTCCCTCGAGCTCCAGGCCAGGACTGAGGAGGAGAA; GAAAGACTGGGTCCAGGCCATCAACTCCACCCTCCTGAAGCATGAACAGACGCTGGAGACTTTCAAACTG; TTGAACTCAACAAACAGGGAAGATGAAGACACCCCACCCAACTCTCCAAACGTGGATCTTGGGAAGCGGG; CACCTACGCCCATCCGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAGGAGCCCTTCAATTCTATCAC; CAAACGCAGGCACCACTGCAAGGCCTGCGGGCATGTGGTTTGTGGGAAGTGCTCCGAGTTCCGGGCCCGC; CTCGTCTATGACAACAACCGCTCCAACCGTGTGTGCACTGATTGCTATGTGGCCTTGCACGGGGTGCCTG; GGAGCAGTCCAGCCTGCAGCCAGCATACACCCCAGCGCCGGAGGTCCATCCTGGAGAAACAGGCCTCAGT; GGCTGCAGAGAACAGCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGGTGGCAAAGGATGGCACAAG; GCATGGTTCGTGGTCCCTGAAAATGAACCCTTGGTGCTGTATATCTACGGAGCCCCTCAGGATGTGAAAG; CCCAGCGCAGCCTGCCCCTCATTGGCTTCGAGGTGGGACCGCCCGAGGCAGGGGAGCGGCCTGACAGAAG; GCATGTCTTCAAGATCACCCAGAGCCACCTCAGCTGGTACTTCAGCCCTGAGACAGAGGAACTACAGCGA; CGCTGGATGGCTGTGCTTGGCCGGGCGGGCCGAGGGGACACGTTCTGCCCGGGGCCCACACTGTCTGAGG; ACAGGGAGATGGAGGAGGCACCGGTGGCTGCTTTAGGAGCCACTGCTGAACCCCCCGAATCCCCCCAGAC; CCGAGACAAGACC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC205133 representing NM_004463; Red=Cloning site Green=Tags(s); ; MHGHRAPGGAGPSEPEHPATNPPGAAPPACADSDPGASEPGLLARRGSGSALGGPLDPQFVGPSDTSLGA; APGHRVLPCGPSPQHHRALRFSYHLEGSQPRPGLHQGNRILVKSLSLDPGQSLEPHPEGPQRLRSDPGPP; TETPSQRPSPLKRAPGPKPQVPPKPSYLQMPRMPPPLEPIPPPPSRPLPADPRVAKGLAPRAEASPSSAA; VSSLIEKFEREPVIVASDRPVPGPSPGPPEPVMLPQPTSQPPVPQLPEGEASRCLFLLAPGPRDGEKVPN; RDSGIDSISSPSNSEETCFVSDDGPPSHSLCPGPPALASVPVALADPHRPGSQEVDSDLEEEDDEEEEEE; KDREIPVPLMERQESVELTVQQKVFHIANELLQTEKAYVSRLHLLDQVFCARLLEEARNRSSFPADVVHG; IFSNICSIYCFHQQFLLPELEKRMEEWDRYPRIGDILQKLAPFLKMYGEYVKNFDRAVELVNTWTERSTQ; FKVIIHEVQKEEACGNLTLQHHMLEPVQRIPRYELLLKDYLLKLPHGSPDSKDAQKSLELIATAAEHSNA; AIRKMERMHKLLKVYELLGGEEDIVSPTKELIKEGHILKLSAKNGTTQDRYLILFNDRLLYCVPRLRLLG; QKFSVRARIDVDGMELKESSNLNLPRTFLVSGKQRSLELQARTEEEKKDWVQAINSTLLKHEQTLETFKL; LNSTNREDEDTPPNSPNVDLGKRAPTPIREKEVTMCMRCQEPFNSITKRRHHCKACGHVVCGKCSEFRAR; LVYDNNRSNRVCTDCYVALHGVPGSSPACSQHTPQRRRSILEKQASVAAENSVICSFLHYMEKGGKGWHK; AWFVVPENEPLVLYIYGAPQDVKAQRSLPLIGFEVGPPEAGERPDRRHVFKITQSHLSWYFSPETEELQR; RWMAVLGRAGRGDTFCPGPTLSEDREMEEAPVAALGATAEPPESPQTRDKT; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004463
• ORF Size: 2883 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004463.3
• RefSeq Size: 4291 bp
• RefSeq ORF: 2886 bp
• Locus ID: 2245
• UniProt ID: P98174
• Cytogenetics: Xp11.22
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 106.4 kDa
• Summary: This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of the faciogenital dysplasia in Aarskog-Scott syndrome and a syndromatic form of X-linked cognitive disability. [provided by RefSeq, Jul 2017]