FGD1 (NM_004463) Human Untagged Clone

SKU SC117376

FGD1 (untagged)-Human FYVE, RhoGEF and PH domain containing 1 (FGD1)

Product Data

• Type: Human Untagged Clone
• Target Symbol: FGD1
• Synonyms: AAS; FGDY; MRXS16; ZFYVE3
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117376 sequence for NM_004463 edited (data generated by NextGen Sequencing); ATGCATGGCCACCGAGCCCCGGGGGGCGCCGGGCCTTCGGAGCCCGAACACCCGGCCACG; AACCCGCCGGGCGCCGCTCCGCCGGCCTGTGCCGACTCGGACCCTGGAGCCTCGGAACCC; GGACTGCTGGCGCGCAGGGGCTCAGGTTCGGCTCTTGGCGGCCCACTGGATCCCCAGTTT; GTCGGACCCTCGGACACCAGCCTGGGCGCTGCTCCAGGCCACCGGGTCTTGCCCTGCGGT; CCCAGTCCACAGCACCACCGGGCCCTGCGCTTCTCTTACCACCTGGAGGGCTCGCAGCCT; CGGCCTGGGCTGCACCAGGGAAACCGGATCCTGGTTAAAAGTTTGTCCCTTGACCCTGGC; CAAAGCCTAGAGCCTCATCCAGAAGGTCCCCAGCGGCTTCGCTCAGACCCAGGTCCCCCG; ACTGAAACCCCTAGCCAGCGTCCTTCACCACTGAAGCGGGCACCGGGCCCGAAGCCACAG; GTGCCCCCAAAGCCCAGCTACCTGCAGATGCCCCGGATGCCCCCCCCACTGGAGCCCATC; CCCCCTCCACCATCACGCCCACTGCCTGCCGACCCCCGAGTGGCCAAGGGCCTGGCTCCC; AGGGCAGAGGCCAGCCCCAGTTCTGCAGCAGTATCCTCACTGATTGAGAAGTTTGAAAGA; GAGCCTGTGATTGTCGCCTCGGATAGACCAGTCCCTGGCCCCAGCCCAGGTCCCCCAGAG; CCAGTCATGTTGCCACAGCCAACCTCGCAGCCACCAGTGCCCCAGCTCCCCGAGGGTGAG; GCCTCCCGCTGCCTGTTTCTGCTGGCTCCTGGGCCCCGGGACGGTGAGAAGGTGCCCAAC; CGGGACAGCGGCATTGATAGCATCAGCTCGCCATCCAACAGCGAGGAGACCTGCTTCGTC; AGTGATGACGGGCCCCCCAGCCACAGCCTCTGCCCTGGGCCCCCTGCCCTGGCTAGTGTG; CCTGTTGCCTTGGCCGACCCCCACCGGCCTGGCTCCCAAGAGGTTGACAGTGACCTGGAG; GAGGAGGACGACGAGGAGGAGGAGGAAGAGAAGGACAGAGAAATCCCAGTGCCCCTGATG; GAGAGACAGGAGTCTGTGGAGTTGACTGTGCAGCAAAAGGTGTTTCACATTGCCAATGAG; CTCCTGCAAACTGAGAAGGCCTACGTTTCCAGGCTCCATCTCCTGGATCAGGTGTTCTGT; GCCCGGCTGCTGGAAGAAGCTCGGAACCGCAGTTCCTTCCCGGCCGACGTTGTCCACGGC; ATCTTCTCTAACATCTGCTCCATCTATTGCTTCCACCAGCAGTTCCTGCTGCCTGAGCTA; GAGAAGCGCATGGAGGAATGGGACCGCTATCCACGCATTGGAGACATCCTGCAGAAACTG; GCCCCCTTCCTCAAGATGTATGGTGAGTATGTGAAGAACTTTGACCGGGCCGTGGAGCTG; GTCAACACCTGGACAGAGCGCTCCACCCAGTTTAAAGTCATCATCCATGAGGTGCAGAAG; GAGGAAGCCTGTGGCAACCTGACATTGCAGCACCACATGCTGGAGCCTGTGCAGCGCATC; CCCCGCTATGAGCTTCTTCTCAAGGACTATCTGTTAAAGCTGCCCCATGGCTCCCCGGAC; AGCAAGGATGCCCAAAAGTCTCTGGAGCTGATCGCCACAGCAGCAGAGCACTCGAATGCT; GCCATCCGCAAAATGGAGCGAATGCATAAGCTGCTGAAGGTATATGAGCTGTTAGGGGGC; GAGGAGGACATTGTCAGCCCCACCAAAGAGCTCATAAAAGAAGGCCACATCCTTAAGCTG; TCAGCAAAGAATGGGACCACTCAAGACCGATACCTCATACTATTCAACGACCGCCTCCTT; TACTGCGTGCCCAGGCTGCGGCTCCTTGGCCAGAAGTTTAGCGTGCGGGCACGCATTGAT; GTAGATGGCATGGAGCTAAAGGAGAGCTCCAACCTCAATCTGCCTCGAACCTTCCTGGTG; TCAGGAAAGCAGCGCTCCCTCGAGCTCCAGGCCAGGACTGAGGAGGAGAAGAAAGACTGG; GTCCAGGCCATCAACTCCACCCTCCTGAAGCATGAACAGACGCTGGAGACTTTCAAACTG; TTGAACTCAACAAACAGGGAAGATGAAGACACCCCACCCAACTCTCCAAACGTGGATCTT; GGGAAGCGGGCACCTACGCCCATCCGGGAAAAGGAAGTCACCATGTGCATGCGCTGCCAG; GAGCCCTTCAATTCTATCACCAAACGCAGGCACCACTGCAAGGCCTGCGGGCATGTGGTT; TGTGGGAAGTGCTCCGAGTTCCGGGCCCGCCTCGTCTATGACAACAACCGCTCCAACCGT; GTGTGCACTGATTGCTATGTGGCCTTGCACGGGGTGCCTGGGAGCAGTCCAGCCTGCAGC; CAGCATACACCCCAGCGCCGGAGGTCCATCCTGGAGAAACAGGCCTCAGTGGCTGCAGAG; AACAGCGTCATCTGCAGCTTCCTGCACTACATGGAGAAGGGTGGCAAAGGATGGCACAAG; GCATGGTTCGTGGTCCCTGAAAATGAACCCTTGGTGCTGTATATCTACGGAGCCCCTCAG; GATGTGAAAGCCCAGCGCAGCCTGCCCCTCATTGGCTTCGAGGTGGGACCGCCCGAGGCA; GGGGAGCGGCCTGACAGAAGGCATGTCTTCAAGATCACCCAGAGCCACCTCAGCTGGTAC; TTCAGCCCTGAGACAGAGGAACTACAGCGACGCTGGATGGCTGTGCTTGGCCGGGCGGGC; CGAGGGGACACGTTCTGCCCGGGGCCCACACTGTCTGAGGACAGGGAGATGGAGGAGGCA; CCGGTGGCTGCTTTAGGAGCCACTGCTGAACCCCCCGAATCCCCCCAGACCCGAGACAAG; ACCTAG; ; Clone variation with respect to NM_004463.2;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004463 unedited; ATATCCCCGCCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG; CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC; GAATTCGGCACGAGGCCGGCCTCGGCTGCGGCGGGAGGAAGAACGCTTCCCAACCAGCCA; GGAGAGCCCAGATCTATTCCCATTCGGGGAGAAAGAAGAGACCCCGCACTTGGATCCGAC; CGACTTCTCGCCCCGGGGCCANNCGGAAGTGGCTCGCCGTCCACCGGAGAGTCCCCGCCG; AGCGTAGCCCTCCAACCCTCGCCCAACCGCAGCCGCATAGACAGCGCTCCCCAGCGGNCC; CCCGGCCCCGCCGAGCCCACTCCCCGCGCGGCTCCGNNCNCGCCCCCNNNCCGCCCCCGN; NCCCCCGCCCCCCCCCCCNNNNNNCCGGCCGCCCCCGGCCCCCCCCCCCCCCCGCCCCCN; CNCCCCCCCGCCCCCCGCCCCCCCGGCCCGCGCCGCCCCGCCCCCCCCCNNNCCGGGGGC; GCCCGCCGCCCCCCCCCCCCCCCCCGGCCCCCCCCNCCCCCCCCCCCCNCCCCCCCCCCC; GCCCCCCCCCCCCCGNGCCCCCGCCGCACTCCGCCCCCCCCCCCCCCCCCCCCCCCCCCC; CCCCCCNCCCCCCCNCGCCCCCCCCCCCCCGCGNNNNCCCCCCCCCCCCCCGCCCCCCGC; CCCCCCCCCCGGCCCGCCCCCNGCGCCGCCCCNCGCCCCCCCCGCGNGCCGCGCCCCGCC; GGNCNCCCCCGCCCCCCCCCCCCCNNCCCCCGCCGNCCCCCCCCCGCCCCCGCCCCCCCC; CCNNNCCCCNCCCGNCNCNGNCGCCCCCCCCGNCCCCCCNCCNNCCCCCCCCCCCCCCCC; CCCGCCCGCCCNCNCCNCCCCCCCCCCCG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_004463
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_004463.2, NP_004454.2
• RefSeq Size: 4291 bp
• RefSeq ORF: 2886 bp
• Locus ID: 2245
• UniProt ID: P98174
• Cytogenetics: Xp11.22
• Protein Pathways: Regulation of actin cytoskeleton
• Summary: This gene encodes a protein that contains Dbl (DH) and pleckstrin (PH) homology domains and is similar to the Rho family of small GTP-binding proteins. The encoded protein specifically binds to the Rho family GTPase Cdc42Hs and can stimulate the GDP-GTP exchange of the isoprenylated form of Cdc42Hs. It also stimulates the mitogen activated protein kinase cascade leading to c-Jun kinase SAPK/JNK1 activation. Defects in this gene are the cause of the faciogenital dysplasia in Aarskog-Scott syndrome and a syndromatic form of X-linked cognitive disability. [provided by RefSeq, Jul 2017]