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MLK3 (MAP3K11) (NM_002419) Human Tagged ORF Clone

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SKU
RC203782
MAP3K11 (Myc-DDK-tagged)-Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
  $776.00
In Stock*
Bulk Requests & Clone Modifications

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Other products for "MLK3 or NM_002419"

Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol MLK3
Synonyms MEKK11; MLK-3; MLK3; PTK1; SPRK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC203782 representing NM_002419
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGCAGCGGGGGTG
GTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGTTATGCCAACCCGGTGTGGAC
AGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTGGCCCTGAGGAAGGGTGACCGTGTGGAGGTG
CTGTCCCGGGACGCAGCCATCTCAGGAGACGAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCA
TCTTCCCGTCCAACTATGTGTCTCGGGGTGGCGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCT
GCGGCTGGAGGAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTGGCGAGGTGAGCTG
GTGGCTGTGAAGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGTGACAGCCGAGAGCGTTCGCCAGG
AGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAGGCTGTGTGCCTGGAGGAGCC
CAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCCCTCAGCCGAGCTCTGGCCGGGCGGCGCGTG
CCTCCCCATGTGCTGGTCAACTGGGCTGTGCAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCC
TGGTGCCCGTCATCCACCGTGATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGACGA
CATGGAGCACAAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATG
AGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCTAAGGGCAGTG
ACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGACCGGGGAGGTGCCATACCGTGGCATTGACTG
CCTTGCTGTGGCCTATGGCGTAGCTGTTAACAAGCTCACACTGCCCATCCCATCCACCTGCCCCGAGCCC
TTCGCACAGCTTATGGCCGACTGCTGGGCGCAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGC
AGCAGTTGGAGGCGCTGGAGGCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGA
AGGCTGGAAGCGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACTACTGAGCCGC
GAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGAGCAGCTGCGGCGGCGCGAGCACC
TGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGAGCTGACGCTGCTGCTGCAGCAGGTGGACCGCGA
GCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGCAGCAAGCTCCGGGCGCGCGACGGCGGCGAG
CGTATCAGCATGCCACTCGACTTCAAGCACCGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGA
GAAACGTCTTCGAGGTCGGGCCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGAGCC
TGCAGAGCCAGGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAGCAATGGAGAGCGG
CGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGGAGAAGGTCCC
GCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTAGGATCTCCTTCCACACCCCC
AGCACTCAATGGTAACCCCCCGCGGCCTAGCCTGGAGCCCGAGGAGCCCAAGAGGCCTGTCCCCGCAGAG
CGCGGTAGCAGCTCTGGGACGCCCAAGCTGATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCT
CGCTGGGCCTTGGCCGCGACCTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGACAAC
ACCCCCCACGCCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC
AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGGTATCCCTGTGGGCCAGCGGT
CAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGGAGGCACTGTCTCACCCCCACCGGGGACATCACG
CTCTGCTCCTGGCACCCCAGGCACCCCACGTTCACCACCCCTGGGCCTCATCAGCCGACCTCGGCCCTCG
CCCCTTCGCAGCCGCATTGATCCCTGGAGCTTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCAC
CACAGCCTGCACCCCGCCGAGCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACC
TGCCAACCCCTTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGGTGCCCAGGCCCCG
TGGGTGCCGGAAGCGGGGCCT

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC203782 representing NM_002419
Red=Cloning site Green=Tags(s)

MEPLKSLFLKSPLGSWNGSGSGGGGGGGGGRPEGSPKAAGYANPVWTALFDYEPSGQDELALRKGDRVEV
LSRDAAISGDEGWWAGQVGGQVGIFPSNYVSRGGGPPPCEVASFQELRLEEVIGIGGFGKVYRGSWRGEL
VAVKAARQDPDEDISVTAESVRQEARLFAMLAHPNIIALKAVCLEEPNLCLVMEYAAGGPLSRALAGRRV
PPHVLVNWAVQIARGMHYLHCEALVPVIHRDLKSNNILLLQPIESDDMEHKTLKITDFGLAREWHKTTQM
SAAGTYAWMAPEVIKASTFSKGSDVWSFGVLLWELLTGEVPYRGIDCLAVAYGVAVNKLTLPIPSTCPEP
FAQLMADCWAQDPHRRPDFASILQQLEALEAQVLREMPRDSFHSMQEGWKREIQGLFDELRAKEKELLSR
EEELTRAAREQRSQAEQLRRREHLLAQWELEVFERELTLLLQQVDRERPHVRRRRGTFKRSKLRARDGGE
RISMPLDFKHRITVQASPGLDRRRNVFEVGPGDSPTFPRFRAIQLEPAEPGQAWGRQSPRRLEDSSNGER
RACWAWGPSSPKPGEAQNGRRRSRMDEATWYLDSDDSSPLGSPSTPPALNGNPPRPSLEPEEPKRPVPAE
RGSSSGTPKLIQRALLRGTALLASLGLGRDLQPPGGPGRERGESPTTPPTPTPAPCPTEPPPSPLICFSL
KTPDSPPTPAPLLLDLGIPVGQRSAKSPRREEEPRGGTVSPPPGTSRSAPGTPGTPRSPPLGLISRPRPS
PLRSRIDPWSFVSAGPRPSPLPSPQPAPRRAPWTLFPDSDPFWDSPPANPFQGGPQDCRAQTKDMGAQAP
WVPEAGP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_002419
ORF Size 2541 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_002419.4
RefSeq Size 3574 bp
RefSeq ORF 2544 bp
Locus ID 4296
UniProt ID Q16584
Cytogenetics 11q13.1
Domains pkinase, SH3, S_TKc, TyrKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways MAPK signaling pathway
MW 92.5 kDa
Summary The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. provided by RefSeq, Jul 2008
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Documents
Resources
"NM_002419" in other vectors (7)
SKU Description Size Price
RC203782L1 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), Myc-DDK-tagged 10 ug
$1,076.00
RC203782L2 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), mGFP tagged 10 ug
$1,076.00
RC203782L3 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), Myc-DDK-tagged 10 ug
$1,076.00
RC203782L4 Lenti ORF clone of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11), mGFP tagged 10 ug
$1,076.00
RG203782 MAP3K11 (tGFP-tagged) - Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11) 10 ug
$789.00 $976.00
SC110949 MAP3K11 (untagged)-Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11) 10 ug
$776.00
SC323524 MAP3K11 (untagged)-Kinase deficient mutant (K144M) of Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11) 10 ug
$776.00

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