MLK3 (MAP3K11) (NM_002419) Human Untagged Clone

SKU SC110949

MAP3K11 (untagged)-Human mitogen-activated protein kinase kinase kinase 11 (MAP3K11)

Product Data

• Type: Human Untagged Clone
• Target Symbol: MLK3
• Synonyms: MEKK11; MLK-3; MLK3; PTK1; SPRK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_002419 edited; ATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGGAATGGCAGTGGC; AGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCAAAGGCAGCGGGT; TATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACGAGCCCAGTGGGCAGGATGAGCTG; GCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGCCATCTCAGGAGAC; GAGGGCTGGTGGGCGGGCCAGGTGGGTGGCCAGGTGGGCATCTTCCCGTCCAACTATGTG; TCTCGGGGTGGCGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGGAGCTGCGGCTGGAG; GAGGTGATCGGCATTGGAGGCTTTGGCAAGGTGTACAGGGGCAGCTGGCGAGGTGAGCTG; GTGGCTGTGAAGGCAGCTCGCCAGGACCCCGATGAGGACATCAGTGTGACAGCCGAGAGC; GTTCGCCAGGAGGCCCGGCTCTTCGCCATGCTGGCACACCCCAACATCATTGCCCTCAAG; GCTGTGTGCCTGGAGGAGCCCAACCTGTGCCTGGTGATGGAGTATGCAGCCGGTGGGCCC; CTCAGCCGAGCTCTGGCCGGGCGGCGCGTGCCTCCCCATGTGCTGGTCAACTGGGCTGTG; CAGATTGCCCGTGGGATGCACTACCTGCACTGCGAGGCCCTGGTGCCCGTCATCCACCGT; GATCTCAAGTCCAACAACATTTTGCTGCTGCAGCCCATTGAGAGTGACGACATGGAGCAC; AAGACCCTGAAGATCACCGACTTTGGCCTGGCCCGAGAGTGGCACAAAACCACACAAATG; AGTGCCGCGGGCACCTACGCCTGGATGGCTCCTGAGGTTATCAAGGCCTCCACCTTCTCT; AAGGGCAGTGACGTCTGGAGTTTTGGGGTGCTGCTGTGGGAACTGCTGACCGGGGAGGTG; CCATACCGTGGCATTGACTGCCTTGCTGTGGCCTATGGCGTAGCTGTTAACAAGCTCACA; CTGCCCATCCCATCCACCTGCCCCGAGCCCTTCGCACAGCTTATGGCCGACTGCTGGGCG; CAGGACCCCCACCGCAGGCCCGACTTCGCCTCCATCCTGCAGCAGTTGGAGGCGCTGGAG; GCACAGGTCCTACGGGAAATGCCGCGGGACTCCTTCCATTCCATGCAGGAAGGCTGGAAG; CGCGAGATCCAGGGTCTCTTCGACGAGCTGCGAGCCAAGGAAAAGGAACTACTGAGCCGC; GAGGAGGAGCTGACGCGAGCGGCGCGCGAGCAGCGGTCACAGGCGGAGCAGCTGCGGCGG; CGCGAGCACCTGCTGGCCCAGTGGGAGCTAGAGGTGTTCGAGCGCGAGCTGACGCTGCTG; CTGCAGCAGGTGGACCGCGAGCGACCGCACGTGCGCCGCCGCCGCGGGACATTCAAGCGC; AGCAAGCTCCGGGCGCGCGACGGCGGCGAGCGTATCAGCATGCCACTCGACTTCAAGCAC; CGCATCACCGTGCAGGCCTCACCCGGCCTTGACCGGAGGAGAAACGTCTTCGAGGTCGGG; CCTGGGGATTCGCCCACCTTTCCCCGGTTCCGAGCCATCCAGTTGGAGCCTGCAGAGCCA; GGCCAGGCATGGGGCCGCCAGTCCCCCCGACGTCTGGAGGACTCAAGCAATGGAGAGCGG; CGAGCATGCTGGGCTTGGGGTCCCAGTTCCCCCAAGCCTGGGGAAGCCCAGAATGGGAGG; AGAAGGTCCCGCATGGACGAAGCCACATGGTACCTGGATTCAGATGACTCATCCCCCTTA; GGATCTCCTTCCACACCCCCAGCACTCAATGGTAACCCCCCGCGGCCTAGCCTGGAGCCC; GAGGAGCCCAAGAGGCCTGTCCCCGCAGAGCGCGGTAGCAGCTCTGGGACGCCCAAGCTG; ATCCAGCGGGCGCTGCTGCGCGGCACCGCCCTGCTCGCCTCGCTGGGCCTTGGCCGCGAC; CTGCAGCCGCCGGGAGGCCCAGGACGCGAGCGCGGGGAGTCCCCGACAACACCCCCCACG; CCAACGCCCGCGCCCTGCCCGACCGAGCCGCCCCCTTCCCCGCTCATCTGCTTCTCGCTC; AAGACGCCCGACTCCCCGCCCACTCCTGCACCCCTGTTGCTGGACCTGGGTATCCCTGTG; GGCCAGCGGTCAGCCAAGAGCCCCCGACGTGAGGAGGAGCCCCGCGGAGGCACTGTCTCA; CCCCCACCGGGGACATCACGCTCTGCTCCTGGCACCCCAGGCACCCCACGTTCACCACCC; CTGGGCCTCATCAGCCGACCTCGGCCCTCGCCCCTTCGCAGCCGCATTGATCCCTGGAGC; TTTGTGTCAGCTGGGCCACGGCCTTCTCCCCTGCCATCACCACAGCCTGCACCCCGCCGA; GCACCCTGGACCTTGTTCCCGGACTCAGACCCCTTCTGGGACTCCCCACCTGCCAACCCC; TTCCAGGGGGGCCCCCAGGACTGCAGGGCACAGACCAAAGACATGGGTGCCCAGGCCCCG; TGGGTGCCGGAAGCGGGGCCTTGA;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002419 unedited; CCCGGTCAGAATTGTATACGACTCATATAGGCGGCCGCGNAATTCGCACGAGGGGGGCCT; AGGACAGGCAGTCTCGGCCCACTGCGGACGCTCCCTCCACCCCCTGCGCAAAAAGACCCA; ACCGGAGTTGAGGCGCTGCCCCTGAAGGCCCCACCTTACACTTGGCTGGGGGCTCGGAGC; CAGGCTCCCAGGACTGCTCCAGAACCGAGGGAAGCTCGGGTCCCTCCAAGCTAGCCATGG; TGAGGCGCCGGAGGCCCCGGGGCCCCACCCCCCCGGCCTGACCACACTGCCCTGGGTGCC; CTCCTCCAGAAGCCCGAGATGCGGGGGGCCGGGAGACAACACTCCTGGCTCCCCAGAGAG; GCGTGGGTCTGGGGCTGAGGGCCAGGGCCCGGATGCCCAGGTTCCGGGACTAGGGCCTTG; GCAGCCAGCGGGGGTGGGGACCACGGGCACCCAGAGAAGGTCCTCCACACATCCCAGCGC; CGGCTCCCGGCCATGGAGCCCTTGAAGAGCCTCTTCCTCAAGAGCCCTCTAGGGTCATGG; AATGGCAGTGGCAGCGGGGGTGGTGGGGGCGGTGGAGGAGGCCGGCCTGAGGGGTCTCCA; AAGGCAGCGGGTTATGCCAACCCGGTGTGGACAGCCCTGTTCGACTACTAGCCCAGTGGG; CAGGATGAGCTGGCCCTGAGGAAGGGTGACCGTGTGGAGGTGCTGTCCCGGGACGCAGCC; ATCTCAGGAGACGAGGGCTGGTGGGCGGGCCAAGTGGGTGGCCAGGTGGGCATCTTCCCG; TNCACTATGTGTCTCGGGGTGGCGGCCCGCCCCCCTGCGAGGTGGCCAGCTTCCAGAAGC; TGCNGCTGGAGGAAGTGAACCGCATTGGAAGCTTTGGCCAAGTGTACAGGA;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002419 unedited; GCGGGCCGCAATCTAGNATCGAGTTTTTTTTTTTTTTTTTTGGGGAGACAGCTTTTGAGT; TTATTTGGCTTCTGGCTTCACTGGATCCCGAGGCTAAGACTCCAACCCTGGCTGGGGCAG; CAGGAAGGCATCCAGAGAGCCCTGGCCCCAGATGACCCCCAGGGCAGGAGGTCCATGCTC; TAAGCCCTAGGGCAGGGGCCGCAGTAGCAGGACTTGGTCAAAAGTGCTGGTGACAGCTGA; GGCCGGCCCCTCTTCCCTGCACCTCCCCTCCTCCCTGCATCACCCCAGCAGGCAATTCCC; TGAGACAGGCTCTGGTCCTCCCAACCAGCTGGGTACAGTGTTGGGCCCCAGTAGGGCAGG; TGAGCTGGGGGGACCTACAGGTTTCAGATGTGGGGGCGCAGGTCCCCCTTCCAGTGTGAA; GGCTTCCTGTGCAGTGTAGTGTTCCTGACCCCCAAAGGGGGGTGGGGTCCCTGGGGAAAC; TGAGGCAGCTGCAGAGGCTGACCCAGCTCCTGTTCCAGTGTATGCTGTGACTCCTCCTAA; GGCAGCTGGAGCTCGGGGGAGTGGCCTGGCCCACTCAAGGCCCCGCTTCCGGCACCCACG; GGGCCTGGGCACCCATGTCTTTGGTCTGTGCCCTGCAGTCCTGGGGGCCCCCCTGGAANG; GGGTTGCANGTGGGGAGTCCCAAAGGGGTCTGAGTCCGGGAACAANGTCCAGGTGCTCGC; GGGTGCAGCTGTGTGATGCAGGGAAAGGCCCTGGCCACTGACCAAGCTCAGGATCATGCG; CTGCAGGGCNAGGCCNAGTCGCTATGAGCCCAGGGTGTGACCTGGGTGCTGGGTGCAGAC; AAACGGAATCCCGTGGGTGAACAGGCCTCCGGGCTCTCTACTGGGGGTTGGCTGACGTGG; CCAGATATCCGTCACACAGGA;
• Restriction Sites: NotI-NotI
• ACCN: NM_002419
• Insert Size: 3860 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_002419.3, NP_002410.1
• RefSeq Size: 3574 bp
• RefSeq ORF: 2544 bp
• Locus ID: 4296
• UniProt ID: Q16584
• Cytogenetics: 11q13.1
• Domains: pkinase, SH3, S_TKc, TyrKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Summary: The protein encoded by this gene is a member of the serine/threonine kinase family. This kinase contains a SH3 domain and a leucine zipper-basic motif. This kinase preferentially activates MAPK8/JNK kinase, and functions as a positive regulator of JNK signaling pathway. This kinase can directly phosphorylate, and activates IkappaB kinase alpha and beta, and is found to be involved in the transcription activity of NF-kappaB mediated by Rho family GTPases and CDC42. [provided by RefSeq, Jul 2008]