Gemin 4 (GEMIN4) (NM_015721) Human Tagged ORF Clone

SKU RC203733

GEMIN4 (Myc-DDK-tagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: Gemin 4
• Synonyms: HC56; HCAP1; HHRF-1; NEDMCR; p97
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC203733 representing NM_015721; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGACCTAGGACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGC; AGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAACGTGTTGGACGGCCCATCGT; GGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTTGCCTGGAAGAAGAAAGCCCTG; ATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTGACCCCGTCCGACACAGAGACACGGTGGCAGG; AAGACCTGTTCTTCTCGGTGGGCAACATGATCCCCACCATCAACCACACCATCCTCTTCGAGCTGCTCAA; ATCCCTGGAAGCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAA; CTAGAGCGCTTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGACGTCT; GGTGGGAGGTGATGAAGCACAAGGGTCACCCGCAGGACCCCCTGCTCTCCCAGTTTAGTGCAATGGCCCA; TAAGTACCTGCCTGCCTTAGATGAGTTCCCCCATCCTCCAAAGAGGCTTAGGTCAGACCCAGACGCGTGC; CCCACCATGCCCCTGTTGGCCATGCTGCTCCGCGGGCTGACACAGATCCAGAGTCGGATCCTGGGCCCGG; GGAGGAAGTGCTGTGCGCTGGCCAACCTGGCTGACATGCTGACTGTGTTTGCGCTGACAGAGGACGACCC; CCAGGAGGTGTCTGCAACCGTGTATCTGGACAAACTGGCCACGGTGATCTCTGTGTGGAACTCGGACACC; CAGAATCCCTACCACCAGCAGGCGCTGGCAGAGAAGGTGAAGGAGGCAGAACGGGATGTCAGCCTGACCT; CGCTGGCCAAACTCCCCAGTGAGACCATTTTCGTGGGCTGCGAGTTCCTGCACCACCTGCTGCGGGAGTG; GGGGGAGGAGTTGCAGGCCGTGCTCCGCAGCAGCCAGGGGACAAGTTACGACAGCTACCGGCTGTGCGAC; AGTCTGACTTCCTTCAGCCAGAACGCGACGCTCTACCTGAACCGCACCAGCCTGTCCAAGGAGGACAGGC; AGGTGGTCTCTGAGCTGGCGGAGTGTGTCAGGGACTTCCTGAGGAAAACGAGCACGGTGCTGAAGAACAG; GGCCTTGGAGGATATCACAGCTTCCATTGCCATGGCCGTCATCCAGCAGAAGATGGACCGCCATATGGAA; GTGTGCTACATTTTTGCCTCTGAGAAGAAGTGGGCCTTCTCGGACGAGTGGGTAGCCTGCCTGGGGAGTA; ACAGGGCCCTCTTCCGACAGCCAGACTTGGTGTTGAGGCTGCTGGAAACAGTGATAGACGTCAGCACAGC; TGACAGAGCCATCCCTGAGTCTCAGATCCGGCAGGTGATCCACCTGATCCTGGAATGTTACGCAGACCTC; TCCCTGCCAGGTAAAAATAAAGTCCTTGCAGGTATCCTGCGTTCCTGGGGGCGAAAGGGCCTCTCTGAAA; AGTTGCTGGCTTATGTGGAGGGTTTTCAGGAAGACCTCAATACAACTTTTAACCAGCTCACTCAGAGTGC; CTCCGAACAGGGCTTGGCAAAAGCTGTGGCCTCCGTGGCCCGCCTGGTCATAGTGCACCCGGAAGTCACG; GTGAAGAAAATGTGCAGCCTGGCTGTGGTCAATCTCGGCACCCACAAGTTCCTGGCCCAGATTCTCACTG; CCTTCCCTGCCCTTAGGTTTGTGGAAGAGCAGGGTCCCAATTCATCTGCCACTTTCATGGTGTCATGCCT; CAAAGAAACCGTCTGGATGAAGTTCTCTACACCCAAGGAAGAAAAGCAATTTTTAGAGCTCCTGAACTGC; CTGATGAGTCCCGTGAAACCCCAAGGGATTCCAGTGGCTGCTCTTCTTGAGCCAGACGAGGTGCTGAAGG; AATTTGTCCTGCCTTTCTTGAGGTTAGATGTTGAAGAGGTAGACCTCAGTCTGAGGATCTTCATCCAGAC; TCTAGAGGCAAACGCGTGCCGAGAGGAATACTGGCTCCAGACCTGCTCCCCGTTTCCACTCCTCTTCAGC; TTGTGCCAGCTCTTGGACCGCTTCAGCAAATACTGGCAGCTTCCCAAGGAGAAGCGGTGCCTCTCTTTGG; ATAGGAAGGATCTAGCGATCCATATCCTGGAGCTCCTGTGTGAGATTGTATCAGCCAATGCTGAGACCTT; CTCCCCGGATGTCTGGATCAAGTCCCTGTCCTGGCTCCACCGCAAGTTAGAACAGCTAGACTGGACTGTG; GGCCTGAGGCTGAAGAGCTTCTTCGAGGGGCACTTCAAGTGTGAAGTGCCAGCCACACTTTTTGAGATCT; GTAAGCTTTCAGAAGACGAGTGGACCTCCCAGGCCCACCCAGGGTACGGGGCTGGCACGGGGCTCCTGGC; CTGGATGGAGTGCTGCTGCGTCTCCAGCGGCATCTCGGAGAGGATGCTGTCTCTCTTGGTGGTGGACGTG; GGCAATCCTGAGGAGGTCAGACTGTTCAGCAAAGGCTTTCTGGTGGCCCTGGTGCAAGTCATGCCTTGGT; GCAGCCCTCAGGAGTGGCAGCGCCTTCACCAGCTGACCAGGAGACTGCTGGAGAAGCAGCTCCTCCATGT; CCCTTATAGCCTGGAATATATTCAGTTTGTTCCCCTGCTCAACCTGAAGCCCTTTGCCCAGGAGTTGCAA; CTCTCCGTCCTCTTCCTGAGGACTTTCCAGTTTCTCTGCAGCCATAGCTGTCGTGATTGGCTTCCTCTGG; AAGGCTGGAACCACGTGGTCAAACTCCTCTGTGGCAGTCTGACCCGCCTCCTGGACTCAGTCAGGGCGAT; ACAGGCAGCTGGCCCTTGGGTTCAAGGACCAGAGCAGGACCTGACCCAGGAAGCCCTGTTTGTTTACACC; CAGGTGTTCTGCCATGCTCTGCACATCATGGCCATGCTCCACCCGGAGGTCTGTGAGCCACTCTACGTTT; TAGCCTTGGAAACCCTCACCTGCTATGAGACTTTGAGCAAGACCAACCCTTCTGTCAGCTCCTTGCTCCA; GAGGGCACACGAGCAGCGCTTCTTAAAGTCCATTGCTGAGGGCATCGGCCCTGAAGAACGGCGCCAAACC; CTGTTGCAGAAGATGAGCAGCTTC; ; AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC; TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC203733 representing NM_015721; Red=Cloning site Green=Tags(s); ; MDLGPLNICEEMTILHGGFLLAEQLFHPKALAELTKSDWERVGRPIVEALREISSAAAHSQPFAWKKKAL; IIIWAKVLQPHPVTPSDTETRWQEDLFFSVGNMIPTINHTILFELLKSLEASGLFIQLLMALPTTICHAE; LERFLEHVTVDTSAEDVAFFLDVWWEVMKHKGHPQDPLLSQFSAMAHKYLPALDEFPHPPKRLRSDPDAC; PTMPLLAMLLRGLTQIQSRILGPGRKCCALANLADMLTVFALTEDDPQEVSATVYLDKLATVISVWNSDT; QNPYHQQALAEKVKEAERDVSLTSLAKLPSETIFVGCEFLHHLLREWGEELQAVLRSSQGTSYDSYRLCD; SLTSFSQNATLYLNRTSLSKEDRQVVSELAECVRDFLRKTSTVLKNRALEDITASIAMAVIQQKMDRHME; VCYIFASEKKWAFSDEWVACLGSNRALFRQPDLVLRLLETVIDVSTADRAIPESQIRQVIHLILECYADL; SLPGKNKVLAGILRSWGRKGLSEKLLAYVEGFQEDLNTTFNQLTQSASEQGLAKAVASVARLVIVHPEVT; VKKMCSLAVVNLGTHKFLAQILTAFPALRFVEEQGPNSSATFMVSCLKETVWMKFSTPKEEKQFLELLNC; LMSPVKPQGIPVAALLEPDEVLKEFVLPFLRLDVEEVDLSLRIFIQTLEANACREEYWLQTCSPFPLLFS; LCQLLDRFSKYWQLPKEKRCLSLDRKDLAIHILELLCEIVSANAETFSPDVWIKSLSWLHRKLEQLDWTV; GLRLKSFFEGHFKCEVPATLFEICKLSEDEWTSQAHPGYGAGTGLLAWMECCCVSSGISERMLSLLVVDV; GNPEEVRLFSKGFLVALVQVMPWCSPQEWQRLHQLTRRLLEKQLLHVPYSLEYIQFVPLLNLKPFAQELQ; LSVLFLRTFQFLCSHSCRDWLPLEGWNHVVKLLCGSLTRLLDSVRAIQAAGPWVQGPEQDLTQEALFVYT; QVFCHALHIMAMLHPEVCEPLYVLALETLTCYETLSKTNPSVSSLLQRAHEQRFLKSIAEGIGPEERRQT; LLQKMSSF; ; SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015721
• ORF Size: 3174 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015721.3
• RefSeq Size: 3757 bp
• RefSeq ORF: 3177 bp
• Locus ID: 50628
• UniProt ID: P57678
• Cytogenetics: 17p13.3
• Protein Families: Druggable Genome
• MW: 120.5 kDa
• Summary: The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]