Gemin 4 (GEMIN4) (NM_015721) Human Untagged Clone

SKU SC111260

GEMIN4 (untagged)-Human gem (nuclear organelle) associated protein 4 (GEMIN4)

Product Data

• Type: Human Untagged Clone
• Target Symbol: Gemin 4
• Synonyms: HC56; HCAP1; HHRF-1; NEDMCR; p97
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC111260 sequence for NM_015721 edited (data generated by NextGen Sequencing); ATGGACCTAGGACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTG; CTGGCCGAGCAGCTGTTCCACCCTAAGGCACTGGCAGAATTAACAAAGTCTGACTGGGAA; CGTGTTGGACGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCC; CAGCCCTTTGCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCG; CACCCCGTGACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTG; GGCAACATGATCCCCACCATCAACCACACCATCCTCTTCGAGCTGCTCAAATCCCTGGAA; GCTTCTGGACTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGCAGAA; CTAGAGCGCTTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTC; CTGGACGTCTGGTGGGAGGTGATGAAGCACAAGGGTCACCCGCAGGACCCCCTGCTCTCC; CAGTTTAGTGCAATGGCCCATAAGTACCTGCCTGCCTTAGATGAGTTCCCCCATCCTCCA; AAGAGGCTTAGGTCAGACCCAGACGCGTGCCCCACCATGCCCCTGTTGGCCATGCTGCTC; CGCGGGCTGACACAGATCCAGAGTCGGATCCTGGGCCCGGGGAGGAAGTGCTGTGCGCTG; GCCAACCTGGCTGACATGCTGACTGTGTTTGCGCTGACAGAGGACGACCCCCAGGAGGTG; TCTGCAACCGTGTATCTGGACAAACTGGCCACGGTGATCTCTGTGTGGAACTCGGACACC; CAGAATCCCTACCACCAGCAGGCGCTGGCAGAGAAGGTGAAGGAGGCAGAACGGGATGTC; AGCCTGACCTCGCTGGCCAAACTCCCCAGTGAGACCATTTTCGTGGGCTGCGAGTTCCTG; CACCACCTGCTGCGGGAGTGGGGGGAGGAGTTGCAGGCCGTGCTCCGCAGCAGCCAGGGG; ACAAGTTACGACAGCTACCGGCTGTGCGACAGTCTGACTTCCTTCAGCCAGAACGCAACG; CTCTACCTGAACCGCACCAGCCTGTCCAAGGAGGACAGGCAGGTGGTCTCTGAGCTGGCG; GAGTGTGTCAGGGACTTCCTGAGGAAAACGAGCACGGTGCTGAAGAACAGGGCCTTGGAG; GATATCACAGCTTCCATTGCCATGGCCGTCATCCAGCAGAAGATGGACCGCCATATGGAA; GTGTGCTACATTTTTGCCTCTGAGAAGAAGTGGGCCTTCTCGGACGAGTGGGTAGCCTGC; CTGGGGAGTAACAGGGCCCTCTTCCGAGAGCCAGACTTGGTGTTGAGGCTGCTGGAAACA; GTGATAGACGTCAGCACAGCTGACAGAGCCATCCCTGAGTCTCAGATCCGGCAGGTGATC; CACCTGATCCTGGAATGTTACGCAGACCTCTCCCTGCCAGGTAAAAATAAAGTCCTTGCA; GGTATCCTGCGTTCCTGGGGGCGAAAGGGCCTCTCTGAAAAGTTGCTGGCTTATGTGGAG; GGTTTTCAGGAAGACCTCAATACAACTTTTAACCAGCTCACTCAGAGTGCCTCCGAACAG; GGCTTGGCAAAAGCTGTGGCCTCCGTGGCCCGCCTGGTCATAGTGCACCCGGAAGTCACG; GTGAAGAAAATGTGCAGCCTGGCTGTGGTCAATCTCGGCACCCACAAGTTCCTGGGCCAG; ATTCTCACTGCCTTCCCTGCCCTTAGGTTTGTGGAAGAGCAGGGTCCCAATTCATCTGCC; ACTTTCATGGTGTCATGCCTCAAAGAAACCGTCTGGATGAAGTTCTCTACACCCAAGGAA; GAAAAGCAATTTTTAGAGCTCCTGAACTGCCTGATGAGTCCCGTGAAACCCCAAGGGATT; CCAGTGGCTGCTCTTCTTGAGCCAGACGAGGTGCTGAAGGAATTTGTCCTGCCTTTCTTG; AGGTTAGATGTTGAAGAGGTAGACCTCAGTCTGAGGATCTTCATCCAGACTCTAGAGGCA; AACGCGTGCCAAGAGGAATACTGGCTCCAGACCTGCTCCCCGTTTCCACTCCTCTTCAGC; TTGTGCCAGCTCTTGGACCGCTTCAGCAAATACTGGCAGCTTCCCAAGGAGAAGCGGTGC; CTCTCTTTGGATAGGAAGGATCTAGCGATCCATATCCTGGAGCTCCTGTGTGAGATTGTA; TCAGCCAATGCTGAGACCTTCTCCCCGGATGTCTGGATCAAGTCCCTGTCCTGGCTCCAC; CGCAAGTTAGAACAGCTAGACTGGACTGTGGGCCTGAGGCTGAAGAGCTTCTTCGAGGGG; CACTTCAAGTGTGAAGTGCCAGCCACACTTTTTGAGATCTGTAAGCTTTCAGAAGACGAG; TGGACCTCCCAGGCCCACCCAGGGTACGGGGCTGGCACGGGGCTCCTGGCCTGGATGGAG; TGCTGCTGCGTCTCCAGCGGCATCTCGGAGAGGATGCTGTCTCTCTTGGTGGTGGACGTG; GGCAATCCTGAGGAGGTCAGACTGTTCAGCAAAGGCTTTCTGGTGGCCCTGGTGCAAGTC; ATGCCTTGGTGCAGCCCTCAGGAGTGGCAGCGCCTTCACCAGCTGACCAGGAGACTGCTG; GAGAAGCAGCTCCTCCATGTCCCTTATAGCCTGGAATATATTCAGTTTGTTCCCCTGCTC; AACCTGAAGCCCTTTGCCCAGGAGTTGCAACTCTCCGTCCTCTTCCTGAGGACTTTCCAG; TTTCTCTGCAGCCATAGCTGTCGTAATTGGCTTCCTCTGGAAGGCTGGAACCACGTGGTC; AAACTCCTCTGTGGCAGTCTGACCCGCCTCCTGGACTCAGTCAGGGCAATACAGGCAGCT; GGCCCTTGGGTTCAAGGACCAGAGCAGGACCTGACCCAGGAAGCCCTGTTTGTTTACACC; CAGGTGTTCTGCCATGCTCTGCACATCATGGCCATGCTCCACCCGGAGGTCTGTGAGCCA; CTCTACGTTTTAGCCTTGGAAACCCTCACCTGCTATGAGACTTTGAGCAAGACCAACCCT; TCTGTCAGCTCCTTGCTCCAGAGGGCACACGAGCAGTGCTTCTTAAAGTCCATTGCTGAG; GGCATCGGCCCTGAAGAACGGCGCCAAACCCTGTTGCAGAAGATGAGCAGCTTCTGA; ; Clone variation with respect to NM_015721.2; 1077 g=>a;1348 c=>g;1736 c=>g;2051 g=>a;2785 g=>a;2868 g=>a;3097 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015721 unedited; NTTCTTACCCCCGCCCGTTGNCGCAATGGGCGGTCTGCGTGTACGGTGGGAGGTCTATAT; AAGCAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGC; CGCGAATTCGTCACGAGGTGCGTCTCCCATCGTGCCGTGCGTCCCGCGCCGCGTTCGAGT; TCTCGGAGGGGAGGGGGCGTTAGCCCCGCGCAGCCGCCGGCGTCGCCGCCATGGACCTAG; GACCCTTGAACATCTGTGAAGAAATGACTATTCTGCATGGAGGCTTCTTGCTGGCCGAGC; AGCTGTTCCACCCTAAGGCACTGGCAGAATTCTACAAAGTCTGACTGGGAACGTGTTGGA; CGGCCCATCGTGGAGGCCTTAAGGGAGATCTCCTCGGCTGCAGCACACTCCCAGCCCTTT; GCCTGGAAGAAGAAAGCCCTGATCATCATCTGGGCCAAGGTTCTGCAGCCGCACCCCGTG; ACCCCGTCCGACACAGAGACACGGTGGCAGGAAGACCTGTTCTTCTCGGTGGGCAACATG; ATCCCCACCATCAACCACACCATCCTCTTCGAGCTGCTCAAATCCCTGGAAGCTTCTGGA; CTCTTTATCCAGCTCCTGATGGCCCTGCCCACCACCATCTGCCATGTAGAACTAGAGCGC; TTTCTGGAACATGTGACCGTTGACACTTCTGCCGAAGACGTGGCCTTCTTCCTGGACGTC; TGGTGGGAGGTGATGAAGCACAAGGGTCACCCGCAGGACCCCCTGCTCTCCCAGTTTAGT; GCAATGGCCCCATAGTACCTGCCTGCCTTAGATGAGNTCCCCCATCCTNCAAGAGGCTTA; NGTCAGACCCANACGCGTGCNCCACCATGCCCCTGTTGCCTGCTGCTNCGCGGGCTGCCC; AGANCCAGAGTCGATCCTGGCCCGNGNNAGAAGTGCTGTGCGCACGCCACCTGNCTGCT;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_015721 unedited; TTTAGTTTTAAAACCTTTTTTTTTGCGAAAAAGGAGAATTTTTATGATAGTTTGTACGTT; ACAAATACCCAAGAAACTATTTTCTTTACACCATTATTGTCATGACTTTTTGTGGACAGT; TTCACATAACTGTAAAGTCCAGGCTGCTCGGGTCTGACCCCTACAGACCTGCCATGTTGA; AGCCCATCTTTTTCGCTCCCGCACAGGTAAGAAGCTGCTGATCTTCTGCAGACCCGCCAT; GTTGGGGCCCAGCTCCCCACGCCAAGTCAGAAGCTGCTCATCTTCTGCAACAGGGTTTGG; CGCCGTTCTTCAGGGCCGATGCCCTCAGCAATGGACTTTAAGAAGCACTGCTCGTGTGCC; CTCTGGAGCAAGGAGCTGACAGAAGGGTTGGTCTTGCTCAAAGTCTCATAGCAGGTGAGG; GTTTCCAAGGCTAAAACGTAGAGTGGCTCACAGACCTCCGGGTGGAGCATGGCCATGATG; TGCAGAGCATGGCAGAACACCTGGGTGTAAACAAACAGGGCTTCCTGGGTCAAGTCCTGC; TCTGGTCCTTGAACCCAAGGGCCAGCTGCCTGTATTGCCCTGACTGAGTCCAGGANGCGG; GTCAGACTGCCACAGAGGAGTTTGACCACGTGGTTCCAGCCTTCCAGAGGAAGCCAATTA; CGACAGCTATGGCTGCAGAGAAACTGGANAGTCCTCNAGAAATAGACGGAGAGTTGCAAC; TCCTGGGCAAAGGGCTTTCAGTTGAGCAGGGGAACAACTGGATATATTCAGGCTATTAGG; GACATGGAGGAGCTGCTTCTTCAGCAGTCTCCTGGTCAGCTGGG;
• Restriction Sites: ECoRI-NOT
• ACCN: NM_015721
• Insert Size: 3700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_015721.1, NP_056536.1
• RefSeq Size: 3472 bp
• RefSeq ORF: 3177 bp
• Locus ID: 50628
• UniProt ID: P57678
• Cytogenetics: 17p13.3
• Protein Families: Druggable Genome
• Summary: The product of this gene is part of a large complex localized to the cytoplasm, nucleoli, and to discrete nuclear bodies called Gemini bodies (gems). The complex functions in spliceosomal snRNP assembly in the cytoplasm, and regenerates spliceosomes required for pre-mRNA splicing in the nucleus. The encoded protein directly interacts with a DEAD box protein and several spliceosome core proteins. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq, Jul 2008]