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PIN1 (NM_006221) Human Tagged ORF Clone

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SKU
RC202543
PIN1 (Myc-DDK-tagged)-Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1)
ORF Plasmid:
DDK tGFP
Lentiviral Particles:
DDK mGFP DDK w/ Puro mGFP w/ Puro
AAV Particle:
DDK
  $150.00
In Stock*
Bulk Requests & Clone Modifications

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Specifications
Specifications
Product Data
Type Human Tagged ORF Clone
Target Symbol PIN1
Synonyms DOD; UBL5
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
ORF Nucleotide Sequence
>RC202543 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCGGACGAGGAGAAGCTGCCGCCCGGCTGGGAGAAGCGCATGAGCCGCAGCTCAGGCCGAGTGTACT
ACTTCAACCACATCACTAACGCCAGCCAGTGGGAGCGGCCCAGCGGCAACAGCAGCAGTGGTGGCAAAAA
CGGGCAGGGGGAGCCTGCCAGGGTCCGCTGCTCGCACCTGCTGGTGAAGCACAGCCAGTCACGGCGGCCC
TCGTCCTGGCGGCAGGAGAAGATCACCCGGACCAAGGAGGAGGCCCTGGAGCTGATCAACGGCTACATCC
AGAAGATCAAGTCGGGAGAGGAGGACTTTGAGTCTCTGGCCTCACAGTTCAGCGACTGCAGCTCAGCCAA
GGCCAGGGGAGACCTGGGTGCCTTCAGCAGAGGTCAGATGCAGAAGCCATTTGAAGACGCCTCGTTTGCG
CTGCGGACGGGGGAGATGAGCGGGCCCGTGTTCACGGATTCCGGCATCCACATCATCCTCCGCACTGAG

ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAGGTTTAA
Protein Sequence
>RC202543 protein sequence
Red=Cloning site Green=Tags(s)

MADEEKLPPGWEKRMSRSSGRVYYFNHITNASQWERPSGNSSSGGKNGQGEPARVRCSHLLVKHSQSRRP
SSWRQEKITRTKEEALELINGYIQKIKSGEEDFESLASQFSDCSSAKARGDLGAFSRGQMQKPFEDASFA
LRTGEMSGPVFTDSGIHIILRTE

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms Chromatograms
Sequencher program is needed, download here
Restriction Sites SgfI-MluI Cloning Scheme for this gene | Plasmid Map
ACCN NM_006221
ORF Size 489 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution Method 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Shipping Ambient
Reference Data
RefSeq NM_006221.4
RefSeq Size 1138 bp
RefSeq ORF 492 bp
Locus ID 5300
UniProt ID Q13526
Cytogenetics 19p13.2
Domains Rotamase, WW
Protein Families Druggable Genome
Protein Pathways RIG-I-like receptor signaling pathway
MW 18.2 kDa
Summary Peptidyl-prolyl cis/trans isomerases (PPIases) catalyze the cis/trans isomerization of peptidyl-prolyl peptide bonds. This gene encodes one of the PPIases, which specifically binds to phosphorylated ser/thr-pro motifs to catalytically regulate the post-phosphorylation conformation of its substrates. The conformational regulation catalyzed by this PPIase has a profound impact on key proteins involved in the regulation of cell growth, genotoxic and other stress responses, the immune response, induction and maintenance of pluripotency, germ cell development, neuronal differentiation, and survival. This enzyme also plays a key role in the pathogenesis of Alzheimer's disease and many cancers. Multiple alternatively spliced transcript variants have been found for this gene.provided by RefSeq, Jun 2011
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"NM_006221" in other vectors (6)
SKU Description Size Price
RC202543L1 Lenti ORF clone of Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), Myc-DDK-tagged 10 ug
$450.00
RC202543L2 Lenti ORF clone of Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), mGFP tagged 10 ug
$450.00
RC202543L3 Lenti ORF clone of Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), Myc-DDK-tagged 10 ug
$450.00
RC202543L4 Lenti ORF clone of Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1), mGFP tagged 10 ug
$450.00
RG202543 PIN1 (tGFP-tagged) - Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1) 10 ug
$489.00
SC116229 PIN1 (untagged)-Human peptidylprolyl cis/trans isomerase, NIMA-interacting 1 (PIN1) 10 ug
$150.00

Plasmid Map for RC202543

Download sequence for

file-download TXT (FASTA format)

file-download Genbank

Cloning scheme for RC202543
Circular map for RC202543

file-alt Citations

RDH10 and ATRA sensitize triple-negative breast cancer to taxane-based chemotherapy through regulation of PIN1
Grainger, Zhang, Yu et al
Research Square (2020)
Snippet: destination vector using the Gateway LR Clonase kit (Invitrogen). Human Myc-DDK tagged PIN1 (CAT#: RC202543) was purchased from Origene. QuickChange II XL Site-Directed Mutagenesis kit (Agilent) was used to generate S71D PIN1 construct
Showing 1-1 of 1 papers.
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