C17orf27 (RNF213) (NM_020954) Human Tagged ORF Clone

SKU RC202317

RNF213 (Myc-DDK-tagged)-Human ring finger protein 213 (RNF213), transcript variant 2

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: C17orf27
• Synonyms: ALO17; C17orf27; KIAA1618; MYMY2; MYSTR; NET57
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202317 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGAGTGTCCTTCGTGCCAGCATGTCTCCAAGGAGGAAACCCCCAAGTTCTGCAGCCAGTGCGGAGAGA; GGCTGCCTCCTGCAGCCCCCATAGCAGATTCTGAGAACAATAACTCCACAATGGCGTCGGCCTCGGAGGG; TGAAATGGAGTGTGGGCAGGAGCTGAAGGAGGAAGGGGGCCCGTGCTTGTTCCCGGGCTCAGACAGTTGG; CAAGAAAACCCCGAGGAGCCCTGTTCCAAAGCCTCCTGGACCGTCCAAGAAAGCAAAAAGAAGAAAAGGA; AGAAGAAAAAGAAGGGGAACAAGTCCGCTTCCTCAGAGCTGGCTTCCTTGCCCCTTTCTCCTGCCAGCCC; CTGTCACCTGACTTTGCTTTCAAACCCATGGCCTCAGGACACAGCCCTGCCCCACAGCCAAGCCCAGCAG; AGTGGCCCCACTGGCCAGCCGAGCCAGCCCCCGGGCACAGCCACCACGCCACTGGAGGGTGACGGCCTCT; CCGCGCCCACCGAGGTTGGCGACAGCCCCCTGCAGGCCCAGGCTTTGGGAGAGGCAGGAGTGGCCACAGG; AAGTGAGGCTCAGAGCAGCCCGCAATTCCAGGACCACACGGAAGGGGAGGACCAGGACGCTTCCATCCCC; TCTGGGGGCAGAGGCCTGTCCCAGGAGGGGACCGGTCCCCCCACCTCTGCTGGTGAAGGCCATTCTAGGA; CTGAAGATGCTGCCCAGGAGCTCCTGTTGCCTGAGTCAAAAGGAGGCAGCTCTGAGCCCGGGACAGAACT; GCAGACCACCGAGCAACAGGCAGGGGCCTCAGCCTCTACGGCAGTTGATGCTGTAGCTGAGCCAGCCAAT; GCAGTTAAAGGGGCCGGGAAGGAAATGAAAGAGAAGACCCAGAGAATGAAACAGCCACCAGCAACCACTC; CTCCTTTCAAAACACACTGCCAGGAAGCTGAGACCAAGACCAAGGACGAGACGGCTGCTGCTGAAGAAAA; AGTCGGTAAAAATGAACAAGGGGAGCCTGAAGACCTCAAGAAGCCAGAGGGGAAGAACAGAAGTGCAGCT; GCTGTGAAAAACGAGAAGGAGCAAAAAAACCAGGAAGCAGATGTCCAGGAAGTGAAGGCAAGCACGCTGA; GCCCGGGTGGAGGAGTCACCGTGTTCTTCCACGCCATCATCTCTCTTCATTTCCCATTCAATCCTGACCT; CCATAAAGTCTTCATCAGAGGAGGAGAAGAATTTGGGGAGTCAAAATGGGACAGCAATATCTGTGAGCTG; CACTACACCAGAGACTTGGGTCATGACCGCGTTCTTGTTGAAGGCATTGTCTGCATTTCCAAGAAGCACC; TAGATAAATACATTCCTTACAAGTACGTCATTTATAATGGGGAATCTTTTGAGTATGAGTTCATTTACAA; GCACCAGCAGAAGAAGGGCGAGTACGTCAACCGCTGTCTGTTCATAAAATCTTCACTTCTGGGCTCAGGA; GACTGGCATCAGTACTATGACATAGTTTATATGAAGCCTCATGGGAGACTCCAGAAAGTCATGAACCACA; TCACAGACGGGCCGAGGAAGGACCTGGTGAAGGGGAAGCAGATTGCCGCTGCGCTCATGCTGGACAGCAC; CTTCAGCATCCTGCAGACCTGGGACACCATCAACCTGAACAGCTTCTTCACCCAGTTCGAGCAGTTTTGC; TTTGTCCTGCAACAGCCTATGATTTATGAAGGACAGGCACAGCTGTGGACCGATTTGCAGTACAGGGAGA; AAGAGGTGAAGAGATACCTGTGGCAACATCTGAAAAAACACGTGGTACCATTGCCGGACGGAAAAAGCAC; GGACTTTTTGCCTGTGGACTGCCCAGTGAGGAGTAAACTGAAAACAGGCCTGATTGTCCTTTTTGTAGTG; GAAAAAATTGAGCTTTTATTAGAAGGCAGCCTGGACTGGTTGTGTCACCTCCTAACCTCAGATGCCAGCT; CACCAGATGAGTTTCACCGTGACCTAAGCCACATCCTTGGGATACCTCAGAGCTGGCGGCTGTACCTGGT; GAACCTGTGCCAAAGATGCATGGACACAAGGACGTACACCTGGCTGGGCGCCCTGCCTGTCCTGCACTGC; TGTATGGAGCTGGCCCCGCGGCACAAGGATGCCTGGAGACAGCCTGAGGACACCTGGGCCGCTCTGGAGG; GACTCTCCTTCTCACCGTTCCGGGAACAAATGCTAGATACGAGTTCCCTACTTCAGTTTATGAGAGAGAA; GCAGCATTTGCTGAGCATAGACGAGCCTCTCTTCCGGTCCTGGTTTAGTCTGCTACCTCTGAGTCACCTG; GTTATGTATATGGAAAACTTCATTGAGCACCTGGGTCGTTTTCCTGCTCATATCCTGGACTGTCTTTCAG; GGATTTACTACCGGCTTCCGGGACTTGAGCAAGTCTTGAATACGCAGGATGTTCAGGATGTTCAGAACGT; TCAGAACATTTTAGAAATGCTGTTGCGACTCCTGGACACTTACCGGGACAAGATTCCCGAGGAGGCCTTG; TCACCATCCTACCTGACTGTGTGTCTGAAACTGCATGAAGCCATCTGCAGCAGCACAAAGCTACTTAAGT; TTTACGAGCTGCCAGCCTTATCTGCCGAGATTGTCTGCAGAATGATTAGACTTCTATCTCTGGTGGATTC; TGCAGGACAGAGAGATGAAACTGGAAATAATTCAGTCCAAACAGTCTTCCAAGGGACCCTTGCTGCTACG; AAAAGGTGGCTCCGAGAAGTTTTTACAAAGAACATGCTCACATCTTCAGGTGCCTCATTCACATACGTCA; AGGAAATTGAGGTCTGGAGGCGGCTGGTGGAAATCCAATTCCCCGCGGAGCATGGCTGGAAGGAGTCGTT; GCTGGGAGACATGGAATGGAGGCTCACAAAGGAGGAACCCCTCTCCCAGATCACTGCCTACTGCAATAGT; TGCTGGGACACCAAAGGCTTAGAGGACAGTGTGGCCAAGACCTTCGAGAAATGCATCATTGAAGCCGTGA; GCTCAGCCTGCCAGGTGAACAATCTCTCCTCCTGGGAAACGGATTCGGGCTCACAGCTGTGTTCTGCCAT; GACCCAGCTAAGGGCTATGAAGCACCCGCTGGGTCTCAGCTCCTCCGCTAACTCAGAGATTGGGAAGTGG; GCACCCTCCTCCCTCGCCAAGGGCAATGGCGCTGAAATC; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202317 protein sequence; Red=Cloning site Green=Tags(s); ; MECPSCQHVSKEETPKFCSQCGERLPPAAPIADSENNNSTMASASEGEMECGQELKEEGGPCLFPGSDSW; QENPEEPCSKASWTVQESKKKKRKKKKKGNKSASSELASLPLSPASPCHLTLLSNPWPQDTALPHSQAQQ; SGPTGQPSQPPGTATTPLEGDGLSAPTEVGDSPLQAQALGEAGVATGSEAQSSPQFQDHTEGEDQDASIP; SGGRGLSQEGTGPPTSAGEGHSRTEDAAQELLLPESKGGSSEPGTELQTTEQQAGASASTAVDAVAEPAN; AVKGAGKEMKEKTQRMKQPPATTPPFKTHCQEAETKTKDETAAAEEKVGKNEQGEPEDLKKPEGKNRSAA; AVKNEKEQKNQEADVQEVKASTLSPGGGVTVFFHAIISLHFPFNPDLHKVFIRGGEEFGESKWDSNICEL; HYTRDLGHDRVLVEGIVCISKKHLDKYIPYKYVIYNGESFEYEFIYKHQQKKGEYVNRCLFIKSSLLGSG; DWHQYYDIVYMKPHGRLQKVMNHITDGPRKDLVKGKQIAAALMLDSTFSILQTWDTINLNSFFTQFEQFC; FVLQQPMIYEGQAQLWTDLQYREKEVKRYLWQHLKKHVVPLPDGKSTDFLPVDCPVRSKLKTGLIVLFVV; EKIELLLEGSLDWLCHLLTSDASSPDEFHRDLSHILGIPQSWRLYLVNLCQRCMDTRTYTWLGALPVLHC; CMELAPRHKDAWRQPEDTWAALEGLSFSPFREQMLDTSSLLQFMREKQHLLSIDEPLFRSWFSLLPLSHL; VMYMENFIEHLGRFPAHILDCLSGIYYRLPGLEQVLNTQDVQDVQNVQNILEMLLRLLDTYRDKIPEEAL; SPSYLTVCLKLHEAICSSTKLLKFYELPALSAEIVCRMIRLLSLVDSAGQRDETGNNSVQTVFQGTLAAT; KRWLREVFTKNMLTSSGASFTYVKEIEVWRRLVEIQFPAEHGWKESLLGDMEWRLTKEEPLSQITAYCNS; CWDTKGLEDSVAKTFEKCIIEAVSSACQVNNLSSWETDSGSQLCSAMTQLRAMKHPLGLSSSANSEIGKW; APSSLAKGNGAEI; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020954
• ORF Size: 3189 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_020954.1
• RefSeq Size: 5337 bp
• RefSeq ORF: 3192 bp
• Locus ID: 57674
• UniProt ID: Q63HN8
• Cytogenetics: 17q25.3
• Protein Families: Druggable Genome, Transcription Factors
• MW: 118.4 kDa
• Summary: This gene encodes a protein containing a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions. The protein also contains an AAA domain, which is associated with ATPase activity. This gene is a susceptibility gene for Moyamoya disease, a vascular disorder of intracranial arteries. This gene is also a translocation partner in anaplastic large cell lymphoma and inflammatory myofibroblastic tumor cases, where a t(2;17)(p23;q25) translocation has been identified with the anaplastic lymphoma kinase (ALK) gene on chromosome 2, and a t(8;17)(q24;q25) translocation has been identified with the MYC gene on chromosome 8. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2011]