TRIM27 (NM_006510) Human Tagged ORF Clone

SKU RC202184

TRIM27 (Myc-DDK-tagged)-Human tripartite motif containing 27 (TRIM27)

Product Data

• Type: Human Tagged ORF Clone
• Target Symbol: TRIM27
• Synonyms: RFP; RNF76
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202184 ORF sequence; Red=Cloning site Blue=ORF Green=Tags(s); ; TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC; GCCGCGATCGCC; ; ATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCG; CAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGC; AGAGACTAACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCAC; CTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGG; GCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGT; GTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAG; GAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGG; GGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGA; GCAGCTGTATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCC; ATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCG; CTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAGGACATTGGGGACACATTGAGCAG; GGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACACCTCCAGATTTGCAAGAGAAAATCCACATTTTT; GCCCAAAAATGTCTATTCTTGACGGAGAGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGA; AAATCCAAGAATTAAGAGAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCC; CAGCCTGATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGACAAC; CCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGGAGACATTATTGGG; AGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAAGACTCAGTGTGCAGAAAAGGTGG; AGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTGTCTTTGTGGTATGGGAAAGAATATTGGGCTCTT; ACCTCCCCAATGACTGCCCTACCCCTGCGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATG; CTGGTGAGGTCTCCTTCTACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTG; TGGGCCTGTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATCTGC; CCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATGGAGACCTCCCCT; ; ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT; ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202184 protein sequence; Red=Cloning site Green=Tags(s); ; MASGSVAECLQQETTCPVCLQYFAEPMMLDCGHNICCACLARCWGTAETNVSCPQCRETFPQRHMRPNRH; LANVTQLVKQLRTERPSGPGGEMGVCEKHREPLKLYCEEDQMPICVVCDRSREHRGHSVLPLEEAVEGFK; EQIQNQLDHLKRVKDLKKRRRAQGEQARAELLSLTQMEREKIVWEFEQLYHSLKEHEYRLLARLEELDLA; IYNSINGAITQFSCNISHLSSLIAQLEEKQQQPTRELLQDIGDTLSRAERIRIPEPWITPPDLQEKIHIF; AQKCLFLTESLKQFTEKMQSDMEKIQELREAQLYSVDVTLDPDTAYPSLILSDNLRQVRYSYLQQDLPDN; PERFNLFPCVLGSPCFIAGRHYWEVEVGDKAKWTIGVCEDSVCRKGGVTSAPQNGFWAVSLWYGKEYWAL; TSPMTALPLRTPLQRVGIFLDYDAGEVSFYNVTERCHTFTFSHATFCGPVRPYFSLSYSGGKSAAPLIIC; PMSGIDGFSGHVGNHGHSMETSP; ; TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Chromatograms: Chromatograms ; Sequencher program is needed, download here
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006510
• ORF Size: 1539 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006510.5
• RefSeq Size: 2969 bp
• RefSeq ORF: 1542 bp
• Locus ID: 5987
• UniProt ID: P14373
• Cytogenetics: 6p22.1
• Domains: PRY, RING, SPRY, zf-B_box
• Protein Families: Druggable Genome, Transcription Factors
• MW: 58.5 kDa
• Summary: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]