TRIM27 (NM_006510) Human Untagged Clone

SKU SC116046

TRIM27 (untagged)-Human tripartite motif containing 27 (TRIM27)

Product Data

• Type: Human Untagged Clone
• Target Symbol: TRIM27
• Synonyms: RFP; RNF76
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_006510 edited; GAATTCGGCACGAGGCCCCCGCAGGCCGGCGCCATGGCCTCCGGGAGTGTGGCCGAGTGC; CTGCAGCAGGAGACCACCTGCCCCGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTC; GACTGCGGCCATAACATCTGTTGCGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACT; AACGTGTCGTGCCCGCAGTGCCGGGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGG; CACCTGGCCAACGTGACCCAACTGGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCC; GGCGGCGAGATGGGCGTGTGCGAGAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAG; GACCAGATGCCCATCTGCGTGGTGTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTG; CTGCCGCTCGAGGAGGCGGTGGAGGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCAT; TTAAAAAGAGTGAAAGATTTAAAGAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCT; GAACTCTTGAGCCTAACCCAGATGGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTG; TATCACTCCTTAAAGGAGCATGAGTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTG; GCCATCTACAATAGCATCAATGGTGCCATCACCCAGTTCTCTTGCAACATCTCCCACCTC; AGCAGCCTGATCGCTCAGCTAGAAGAGAAGCAGCAGCAGCCCACCAGGGAGCTCCTGCAG; GACATTGGGGACACATTGAGCAGGGCTGAAAGAATCAGGATTCCTGAACCTTGGATCACA; CCTCCAGATTTGCAAGAGAAAATCCACATTTTTGCCCAAAAATGTCTATTCTTGACGGAG; AGTCTAAAGCAGTTCACAGAAAAAATGCAGTCAGATATGGAGAAAATCCAAGAATTAAGA; GAGGCTCAGTTATACTCAGTGGACGTGACTCTGGACCCAGACACGGCCTACCCCAGCCTG; ATCCTCTCTGATAATCTGCGGCAAGTGCGGTACAGTTACCTCCAACAGGACCTGCCTGAC; AACCCCGAGAGGTTCAATCTGTTTCCCTGTGTCTTGGGCTCTCCATGCTTCATCGCCGGG; AGACATTATTGGGAGGTAGAGGTGGGAGATAAAGCCAAGTGGACCATAGGTGTCTGTGAA; GACTCAGTGTGCAGAAAAGGTGGAGTAACCTCAGCCCCCCAGAATGGATTCTGGGCAGTG; TCTTTGTGGTATGGGAAAGAATATTGGGCTCTTACCTCCCCAATGACTGCCCTACCCCTG; CGGACCCCGCTCCAGCGGGTGGGGATTTTCTTGGACTATGATGCTGGTGAGGTCTCCTTC; TACAACGTGACAGAGAGGTGTCACACCTTCACTTTCTCTCATGCTACCTTTTGTGGGCCT; GTCCGGCCCTACTTCAGTCTGAGTTACTCGGGAGGGAAAAGTGCAGCTCCTCTGATCATC; TGCCCCATGAGTGGGATAGATGGGTTTTCTGGCCATGTTGGGAATCATGGTCATTCCATG; GAGACCTCCCCTTGAGGAGGTGAATTCAGGCCAAAAGGGCTGTTGGCTGTAATCCTACGC; CAGGCACAAGGCATCTTGTTGCCTTGCCACGTCCTGTCACAGCTGGGTATCCTTACCATG; TTCCACGCCCTTGCAGTGGGAGACAGGATGTCCATGTTCTCTACCATCCTTTTCCTTCCC; ATGCAGATTGTGAAATGTAATGAGATGTATCAAGATATCCTAGAAATAAAAACCAGATGT; CCACCTCCAGTGTTTCATACTTTCTGGTTTTACACATCGCTGGAGGGATAAAGAGTATGG; ATAATCTTTGGATTTGGAGAGCCGTTCAAGATACTTCCAGCTTCTTGGCTCAGCCTGGCT; TCCTCTGGTTCAGCCCCACATAATGATTATGGCTATTTGCTGTCATTTCTGGGCTAGGGC; TCCTTTCTAACAACCTAGACTGGAATAAGGCCCTGTCAGCATGGCTCCCTTTATCCCAGT; TTTCCGTCTGGGAACAGTACCTCTGCCCCTGATTCCCAATGTGCCATAGTTTTATTAACT; CCATTAAAGAAGCCTGTATGTGTTTTGGTTAGTTACAGTTATTTTACAATAATGGTGGGT; AATGGCCCCACCTCTGTTATGAGATAATGTTCTAATCAATGTCTCTGCCTTTGTATCTTT; TCTGAGGGCTTTGTCTGTTCTCTTCATTCTAATGAAAGGTGTATTCTAGTGCTGGGTGCA; TATCATCCAGGATAATATTCTGCCCAACTCCATCCTCTGTTACTAGATCCCTTACCAGTC; ACATTTGTGGACTGGTGGCCAGTCGTATACCATCCCTGGAAGGATTCTGGGACAATATTC; CAGGGATTCATTGACTTCTTGGCTCCTTTTCTCCATTTCCTTTGGGGGAAGGGGGAATTG; ACCATGCTTAAGTGCATCCTATCAAGGGGCAGCTCCGTCCCCATGGCCATTGGATCATGA; GACACTCTGAAGTCAGAAGGCTGGGGCAGATCACTTCAAGCAAGCCCCCATGATGGTTCT; CAGTCCTGCTTCTCTGTGGGTACGTGCCCCTCTGTTTAAAAATAAACTGAATATGGATGT; TTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006510 unedited; GGGTACTATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGGCCCCCGCA; GGCCGGCGCCATGGCCTCCGGGAGTGTGGCCGAGTGCCTGCAGCAGGAGACCACCTGCCC; CGTGTGCCTGCAGTACTTCGCAGAGCCCATGATGCTCGACTGCGGCCATAACATCTGTTG; CGCGTGCCTCGCCCGCTGCTGGGGCACGGCAGAGACTAACGTGTCGTGCCCGCAGTGCCG; GGAGACCTTCCCGCAGAGGCACATGCGGCCCAACCGGCACCTGGCCAACGTGACCCAACT; GGTAAAGCAGCTGCGCACCGAGCGGCCGTCGGGGCCCGGCGGCGAGATGGGCGTGTGCGA; GAAGCACCGCGAGCCCCTGAAGCTGTACTGCGAGGAGGACCAGATGCCCATCTGCGTGGT; GTGCGACCGCTCCCGCGAGCACCGCGGCCACAGCGTGCTGCCGCTCGAGGAGGCGGTGGA; GGGCTTCAAGGAGCAAATCCAGAACCAGCTCGACCATTTAAAAAGAGTGAAAGATTTAAA; GAAGAGACGTCGGGCCCAGGGGGAACAGGCACGAGCTGAACTCTTGAGCCTAACCCAGAT; GGAGAGGGAGAAGATTGTTTGGGAGTTTGAGCAGCTGTATCACTCCTTAAAGGAGCATGA; GTATCGCCTCCTGGCCCGCCTTGAGGAGCTAGACTTGGCCATCTACAATAGCATCAATGG; TGCCATCACCCCAGTTCTCTTGCAACATCTCCCACCTCAGCAGCCTGATCGCTCACCTAG; AAGAGAAGCAGCACCAGCCCACCCAGGAGCTCCTGCCAGACCATTGGGGACACATTGAGC; AGGGCTGAAAGGATACGGATCCTGAACCTTGAATACACCTCCAGATTGCAAGAGAAATAC; CCATTTTGCCC;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006510 unedited; TTTTAAAAANNCCTCTGTGNACCGCGCCGCATTCTANGATCGATNTTTTTTTTTTTTTTT; TTTTTTTTTTTTTAAACATCCATATTCAGTTTATTTTTAAACAGAGGGGCACGTACCCAC; AGAGAAGCAGGACTGAAAACCATCATGGGGGCTTGCTTGAAGTGATCTGCCCCAGCCTTC; TGACTTCAAAGTGTCTCATGATCCAATGGCCATGGGGACGGAGCTGCCCCTTGATAGGAT; GCACTTAAGCATGGTCAATTCCCCCTTCCCCCAAAGGAAATGGAGAAAAGGAGCCAAGAA; GTCAATGAATCCCCTGGAATATTGTCCCAGAATCCTTCCAGGGATGGTATACGACTGGCC; ACCAGTCCACAAATGTGACTGGGTAGGGATCTAGTAACAGAGGATGGAGTTGGGCAGAAT; ATTATCCTGGATGATATGCACCCAGCACTAGAATACACCTTTCATTAGAATGAAGAGAAC; GACAAAGCCCTCAAAAAAGATACAAAAGCAAAGACATTGATTAGAACATTATCTCATAAC; AGAAGTTGGGGCCATTACCCACCATTATTGGAAAATACCTGGAAACTAACCAAAACCCTT; ACAAGGCTTCTTTTNTGGGAGTGTAATAAAACTATTGGCCCATTGGNGAAATCAGGGGCC; AAAAGTACTTGTTCCCCNACCGGAAAACCTGGGATAAAGGGGAACCCTTCCTGAAAGGGC; CCTTATTCTCAGTCTTAGGGTTGTTTAAAGAGAGACCCTACCCCCANAAATGCACCCCAA; ATATCCTTTATTCCTTATGTGGGGCGCTGAACCCAAGAGAAACCACGGTTTACCCCAAAA; ACCTGGAATTTTCTTAAACGGCCTCTCACAACCCAAAGATTTTCCATCCTTTTTATTCCC; TTCCCCGATGGTGTAAACCCCAAAAATTGAAAACCTGGGGGGGGGCCACTGGGGTTTTTA; TTTGAGAGATTTGGGACCCTTTTTTAACTTTAACAATTGGGGGGGAAGAGAAAGAGGGGG; NAAAAAATTGGCCTTTCTTCCCCCTTGAGAGGGGG;
• Restriction Sites: NotI-NotI
• ACCN: NM_006510
• Insert Size: 2770 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_006510.3, NP_006501.1
• RefSeq Size: 2984 bp
• RefSeq ORF: 1542 bp
• Locus ID: 5987
• UniProt ID: P14373
• Cytogenetics: 6p22.1
• Domains: PRY, RING, SPRY, zf-B_box
• Protein Families: Druggable Genome, Transcription Factors
• Summary: This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein localizes to the nuclear matrix. It interacts with the enhancer of polycomb protein and represses gene transcription. It is also thought to be involved in the differentiation of male germ cells. Fusion of the N-terminus of this protein with the truncated C-terminus of the RET gene product has been shown to result in production of the ret transforming protein. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (alpha) lacks 280 nt in the coding region, resulting in a frameshift and use of an upstream stop codon. It encodes isoform alpha which has a distinct C-terminus as compared to isoform beta.