OBFC1 (STN1) (NM_024928) Human Tagged ORF Clone

CAT#: RC200778

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OBFC1 (Myc-DDK-tagged)-Human oligonucleotide/oligosaccharide-binding fold containing 1 (OBFC1)



  "NM_024928" in other vectors (4)

Reconstitution Protocol

USD 457.00

In Stock*

Size
    • 10 ug

Product images

Frequently bought together (4)
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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol STN1
Synonyms AAF-44; AAF44; bA541N10.2; OBFC1; RPA-32
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC200778 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGCAGCCTGGATCCAGCCGGTGTGAAGAGGAGACCCCTTCCCTCTTGTGGGGTTTGGATCCTGTGTTTC
TAGCCTTTGCAAAACTCTACATCAGGGATATCCTGGACATGAAGGAGTCCCGCCAGGTGCCAGGTGTATT
TTTGTACAATGGACATCCAATAAAACAGGTAGATGTCTTGGGAACTGTCATTGGAGTGAGAGAAAGAGAT
GCTTTCTACAGTTATGGAGTGGATGACAGCACTGGAGTTATAAACTGCATCTGCTGGAAAAAGTTGAATA
CTGAGTCTGTATCAGCTGCTCCTAGTGCAGCAAGAGAGCTCAGCTTAACCTCACAACTTAAGAAGCTACA
AGAGACCATTGAGCAGAAAACAAAGATAGAGATCGGGGACACGATCCGAGTCAGAGGCAGTATCCGCACA
TACAGAGAAGAGCGAGAGATTCATGCCACCGCTTACTATAAAGTGGACGACCCAGTGTGGAACATTCAAA
TTGCAAGGATGCTTGAGCTGCCCACTATCTACAGGAAAGTTTATGACCAGCCTTTTCACAGCTCAGCCCT
AGAGAAAGAAGAGGCACTAAGCAATCCAGGCGCCCTGGACCTCCCCAGTCTCACGAGTTTGCTGAGTGAA
AAAGCCAAAGAATTCCTCATGGAGAACAGAGTGCAGAGCTTTTACCAGCAGGAGCTGGAAATGGTGGAGT
CTTTGCTGTCCCTTGCCAATCAGCCTGTGATTCACAGTGCCTGCTCCGACCAAGTGAATTTTAAGAAGGA
CACCACTTCCAAGGCAATTCATAGTATATTTAAGAATGCTATACAACTGCTGCAGGAAAAAGGACTTGTT
TTCCAGAAAGATGATGGTTTTGATAACCTATACTATGTAACCAGAGAAGACAAAGACCTGCACAGAAAGA
TCCACCGGATCATTCAGCAGGACTGCCAGAAACCAAATCACATGGAGAAGGGCTGTCACTTCCTGCACAT
CTTGGCCTGTGCTCGCCTGAGCATCCGCCCGGGCCTGAGCGAGGCTGTGCTGCAGCAAGTTCTGGAGCTC
CTGGAGGACCAGAGTGACATTGTCAGCACAATGGAGCACTACTACACAGCGTTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC200778 protein sequence
Red=Cloning site Green=Tags(s)

MQPGSSRCEEETPSLLWGLDPVFLAFAKLYIRDILDMKESRQVPGVFLYNGHPIKQVDVLGTVIGVRERD
AFYSYGVDDSTGVINCICWKKLNTESVSAAPSAARELSLTSQLKKLQETIEQKTKIEIGDTIRVRGSIRT
YREEREIHATAYYKVDDPVWNIQIARMLELPTIYRKVYDQPFHSSALEKEEALSNPGALDLPSLTSLLSE
KAKEFLMENRVQSFYQQELEMVESLLSLANQPVIHSACSDQVNFKKDTTSKAIHSIFKNAIQLLQEKGLV
FQKDDGFDNLYYVTREDKDLHRKIHRIIQQDCQKPNHMEKGCHFLHILACARLSIRPGLSEAVLQQVLEL
LEDQSDIVSTMEHYYTAF

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_024928
ORF Size 1104 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_024928.3, NP_079204.1
RefSeq Size 6483 bp
RefSeq ORF 1107 bp
Locus ID 79991
UniProt ID Q9H668
Cytogenetics 10q24.33
MW 42.1 kDa
Gene Summary OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]

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