OBFC1 (STN1) Mouse Monoclonal Antibody [Clone ID: OTI2E4]

CAT#: TA503240

OBFC1 mouse monoclonal antibody, clone OTI2E4 (formerly 2E4)

Product Images

HEK293T cells were transfected with the pCMV6-ENTRY control (Cat# PS100001, Left lane) or pCMV6-ENTRY OBFC1 (Cat# RC200778, Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-OBFC1(Cat# TA503240). Positive lysates LY410967 (100ug) and LC410967 (20ug) can be purchased separately from OriGene.

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  Western blot analysis of extracts (10ug) from 4 different cell lines by using anti-OBFC1 monoclonal antibody at 1:200 dilution.

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  Anti-OBFC1 mouse monoclonal antibody (TA503240) immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY OBFC1 (RC200778).

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  HEK293T cells transfected with either RC200778 overexpress plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-OBFC1 antibody (TA503240), and then analyzed by flow cytometry.

Specifications

Product Data

• Clone Name: OTI2E4
• Applications: FC, IF, WB
• Recommended Dilution: WB 1:2000, IF 1:100, FLOW 1:100
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Full length human recombinant protein of human OBFC1 (NP_079204) produced in HEK293T cell.
• Formulation: PBS (pH 7.3) containing 1% BSA, 50% glycerol and 0.02% sodium azide.
• Concentration: 0.4 mg/ml
• Purification: Purified from mouse ascites fluids or tissue culture supernatant by affinity chromatography (protein A/G)
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 41.9 kDa
• Gene Name: STN1 subunit of CST complex
• Database Link:
  NP_079204
  Entrez Gene 79991 Human
  UniProt ID Q9H668
• Background: OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]). [supplied by OMIM]
• Synonyms: AAF-44; AAF44; bA541N10.2; RPA-32; STN1