OBFC1 (STN1) (NM_024928) Human Tagged ORF Clone

CAT#: RG200778

OBFC1 (tGFP-tagged) - Human oligonucleotide/oligosaccharide-binding fold containing 1 (OBFC1)

Product Images

Circular map for RG200778

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: OBFC1
• Synonyms: AAF-44; AAF44; bA541N10.2; OBFC1; RPA-32
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG200778 representing NM_024928
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGCCTGGATCCAGCCGGTGTGAAGAGGAGACCCCTTCCCTCTTGTGGGGTTTGGATCCTGTGTTTC
  TAGCCTTTGCAAAACTCTACATCAGGGATATCCTGGACATGAAGGAGTCCCGCCAGGTGCCAGGTGTATT
  TTTGTACAATGGACATCCAATAAAACAGGTAGATGTCTTGGGAACTGTCATTGGAGTGAGAGAAAGAGAT
  GCTTTCTACAGTTATGGAGTGGATGACAGCACTGGAGTTATAAACTGCATCTGCTGGAAAAAGTTGAATA
  CTGAGTCTGTATCAGCTGCTCCTAGTGCAGCAAGAGAGCTCAGCTTAACCTCACAACTTAAGAAGCTACA
  AGAGACCATTGAGCAGAAAACAAAGATAGAGATCGGGGACACGATCCGAGTCAGAGGCAGTATCCGCACA
  TACAGAGAAGAGCGAGAGATTCATGCCACCGCTTACTATAAAGTGGACGACCCAGTGTGGAACATTCAAA
  TTGCAAGGATGCTTGAGCTGCCCACTATCTACAGGAAAGTTTATGACCAGCCTTTTCACAGCTCAGCCCT
  AGAGAAAGAAGAGGCACTAAGCAATCCAGGCGCCCTGGACCTCCCCAGTCTCACGAGTTTGCTGAGTGAA
  AAAGCCAAAGAATTCCTCATGGAGAACAGAGTGCAGAGCTTTTACCAGCAGGAGCTGGAAATGGTGGAGT
  CTTTGCTGTCCCTTGCCAATCAGCCTGTGATTCACAGTGCCTGCTCCGACCAAGTGAATTTTAAGAAGGA
  CACCACTTCCAAGGCAATTCATAGTATATTTAAGAATGCTATACAACTGCTGCAGGAAAAAGGACTTGTT
  TTCCAGAAAGATGATGGTTTTGATAACCTATACTATGTAACCAGAGAAGACAAAGACCTGCACAGAAAGA
  TCCACCGGATCATTCAGCAGGACTGCCAGAAACCAAATCACATGGAGAAGGGCTGTCACTTCCTGCACAT
  CTTGGCCTGTGCTCGCCTGAGCATCCGCCCGGGCCTGAGCGAGGCTGTGCTGCAGCAAGTTCTGGAGCTC
  CTGGAGGACCAGAGTGACATTGTCAGCACAATGGAGCACTACTACACAGCGTTC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG200778 representing NM_024928
  Red=Cloning site Green=Tags(s)
  
  MQPGSSRCEEETPSLLWGLDPVFLAFAKLYIRDILDMKESRQVPGVFLYNGHPIKQVDVLGTVIGVRERD
  AFYSYGVDDSTGVINCICWKKLNTESVSAAPSAARELSLTSQLKKLQETIEQKTKIEIGDTIRVRGSIRT
  YREEREIHATAYYKVDDPVWNIQIARMLELPTIYRKVYDQPFHSSALEKEEALSNPGALDLPSLTSLLSE
  KAKEFLMENRVQSFYQQELEMVESLLSLANQPVIHSACSDQVNFKKDTTSKAIHSIFKNAIQLLQEKGLV
  FQKDDGFDNLYYVTREDKDLHRKIHRIIQQDCQKPNHMEKGCHFLHILACARLSIRPGLSEAVLQQVLEL
  LEDQSDIVSTMEHYYTAF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024928
• ORF Size: 1104 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024928.3, NP_079204.1
• RefSeq Size: 1451 bp
• RefSeq ORF: 1107 bp
• Locus ID: 79991
• UniProt ID: Q9H668
• Cytogenetics: 10q24.33
• Gene Summary: OBFC1 and C17ORF68 (MIM 613129) are subunits of an alpha accessory factor (AAF) that stimulates the activity of DNA polymerase-alpha-primase (see MIM 176636), the enzyme that initiates DNA replication (Casteel et al., 2009 [PubMed 19119139]). OBFC1 also appears to function in a telomere-associated complex with C17ORF68 and TEN1 (C17ORF106; MIM 613130) (Miyake et al., 2009 [PubMed 19854130]).[supplied by OMIM, Nov 2009]