USP14 (NM_005151) Human Tagged ORF Clone

CAT#: RC200337

USP14 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (USP14), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY417492) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC200337 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified USP14 protein (Cat# TP300337). The protein was produced from HEK293T cells transfected with USP14 cDNA clone (Cat# RC200337) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP14
• Synonyms: TGT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC200337 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCCGCTCTACTCCGTTACTGTAAAATGGGGAAAGGAGAAATTTGAAGGTGTAGAATTGAATACAGATG
  AACCTCCAATGGTATTCAAGGCTCAGCTGTTTGCGTTGACTGGAGTCCAGCCTGCCAGACAGAAAGTTAT
  GGTGAAAGGAGGAACGCTAAAGGATGATGATTGGGGAAACATCAAAATAAAAAATGGAATGACTCTACTA
  ATGATGGGGTCAGCAGATGCTCTTCCAGAAGAACCCTCAGCCAAAACTGTCTTCGTAGAAGACATGACAG
  AAGAACAGTTAGCATCTGCTATGGAGTTACCATGTGGATTGACAAACCTTGGTAACACTTGTTACATGAA
  TGCCACAGTTCAGTGTATTCGTTCTGTGCCTGAACTCAAAGATGCCCTTAAAAGGTATGCAGGTGCCTTG
  AGAGCTTCAGGGGAAATGGCTTCAGCGCAGTATATTACTGCAGCCCTTAGAGATTTGTTTGATTCCATGG
  ATAAAACTTCTTCCAGTATTCCACCTATTATTCTACTGCAGTTTTTGCACATGGCTTTCCCACAGTTTGC
  CGAGAAAGGTGAACAAGGACAGTATCTTCAACAGGATGCTAATGAATGTTGGATACAAATGATGCGAGTA
  TTGCAACAGAAATTGGAAGCAATAGAGGATGATTCTGTTAAAGAGACAGACTCCTCATCTGCATCGGCAG
  CGACACCTTCTAAAAAGAAAAGTTTAATCGATCAGTTCTTCGGTGTTGAGTTTGAAACTACCATGAAATG
  TACAGAATCTGAAGAAGAAGAAGTCACCAAAGGAAAGGAAAATCAACTTCAGCTTAGCTGTTTTATCAAT
  CAGGAAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAAGAAATCACCAAACAGTCTCCAA
  CGTTGCAAAGAAATGCCTTGTATATCAAATCTTCCAAGATCAGCCGGCTGCCTGCTTACTTGACCATTCA
  GATGGTTCGATTTTTTTATAAAGAGAAGGAATCTGTGAATGCCAAAGTTCTTAAGGATGTTAAATTTCCT
  CTTATGTTGGATATGTATGAACTGTGTACACCAGAACTTCAAGAGAAAATGGTGTCTTTTCGATCCAAAT
  TCAAGGATCTAGAAGATAAAAAAGTGAATCAGCAGCCAAATACAAGTGACAAAAAGAGTAGTCCCCAGAA
  AGAAGTTAAGTATGAACCCTTTTCTTTTGCTGATGATATTGGCTCCAATAATTGTGGATACTATGACTTA
  CAAGCAGTACTAACACACCAGGGAAGGTCTAGTTCTTCAGGTCATTATGTATCATGGGTGAAAAGGAAAC
  AAGATGAATGGATTAAGTTTGATGATGACAAAGTCAGCATCGTAACACCAGAAGATATCTTACGGCTTTC
  TGGTGGTGGAGACTGGCATATCGCTTACGTTCTACTCTATGGGCCTCGCAGAGTTGAAATAATGGAAGAG
  GAAAGTGAACAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC200337 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MPLYSVTVKWGKEKFEGVELNTDEPPMVFKAQLFALTGVQPARQKVMVKGGTLKDDDWGNIKIKNGMTLL
  MMGSADALPEEPSAKTVFVEDMTEEQLASAMELPCGLTNLGNTCYMNATVQCIRSVPELKDALKRYAGAL
  RASGEMASAQYITAALRDLFDSMDKTSSSIPPIILLQFLHMAFPQFAEKGEQGQYLQQDANECWIQMMRV
  LQQKLEAIEDDSVKETDSSSASAATPSKKKSLIDQFFGVEFETTMKCTESEEEEVTKGKENQLQLSCFIN
  QEVKYLFTGLKLRLQEEITKQSPTLQRNALYIKSSKISRLPAYLTIQMVRFFYKEKESVNAKVLKDVKFP
  LMLDMYELCTPELQEKMVSFRSKFKDLEDKKVNQQPNTSDKKSSPQKEVKYEPFSFADDIGSNNCGYYDL
  QAVLTHQGRSSSSGHYVSWVKRKQDEWIKFDDDKVSIVTPEDILRLSGGGDWHIAYVLLYGPRRVEIMEE
  ESEQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005151
• ORF Size: 1482 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005151.4
• RefSeq Size: 4156 bp
• RefSeq ORF: 1485 bp
• Locus ID: 9097
• UniProt ID: P54578
• Cytogenetics: 18p11.32
• Domains: UBQ, UCH
• Protein Families: Druggable Genome, Protease
• MW: 56.1 kDa
• Gene Summary: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]