USP14 (NM_005151) Human Tagged ORF Clone Lentiviral Particle

CAT#: RC200337L3V

Lenti ORF particles, USP14 (Myc-DDK tagged) - Human ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (USP14), transcript variant 1, 200ul, >10^7 TU/mL

Product Images

RC200337L3 was used to prepare Lentiviral particles using TR30037 packaging kit. HEK293T cells were transduced with RC200337L3V particle to overexpress human USP14-Myc-DDK fusion protein.

Specifications

Product Data

• Type: Human Tagged ORF Clone Lentiviral Particle
• Tag: Myc-DDK
• Symbol: USP14
• Synonyms: TGT
• Mammalian Cell Selection: Puromycin
• Vector: pLenti-C-Myc-DDK-P2A-Puro
• ACCN: NM_005151
• ORF Size: 1482 bp
• Sequence Data:
  ORF Nucleotide Sequence

  The ORF insert of this clone is exactly the same as(RC200337).
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_005151.3
• RefSeq Size: 4156 bp
• RefSeq ORF: 1485 bp
• Locus ID: 9097
• UniProt ID: P54578
• Cytogenetics: 18p11.32
• Domains: UBQ, UCH
• Protein Families: Druggable Genome, Protease
• MW: 56.1 kDa
• Gene Summary: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]