USP14 (NM_005151) Human Untagged Clone

SKU SC116911

USP14 (untagged)-Human ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (USP14), transcript variant 1

Product Data

• Type: Human Untagged Clone
• Target Symbol: USP14
• Synonyms: TGT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116911 sequence for NM_005151 edited (data generated by NextGen Sequencing); ATGCCGCTCTACTCCGTTACTGTAAAATGGGGAAAGGAGAAATTTGAAGGTGTAGAATTG; AATACAGATGAACCTCCAATGGTATTCAAGGCTCAGCTGTTTGCGTTGACTGGAGTCCAG; CCTGCCAGACAGAAAGTTATGGTGAAAGGAGGAACGCTAAAGGATGATGATTGGGGAAAC; ATCAAAATAAAAAATGGAATGACTCTACTAATGATGGGGTCAGCAGATGCTCTTCCAGAA; GAACCCTCAGCCAAAACTGTCTTCGTAGAAGACATGACAGAAGAACAGTTAGCATCTGCT; ATGGAGTTACCATGTGGATTGACAAACCTTGGTAACACTTGTTACATGAATGCCACAGTT; CAGTGTATTCGTTCTGTGCCTGAACTCAAAGATGCCCTTAAAAGGTATGCAGGTGCCTTG; AGAGCTTCAGGGGAAATGGCTTCAGCGCAGTATATTACTGCAGCCCTTAGAGATTTGTTT; GATTCCATGGATAAAACTTCTTCCAGTATTCCACCTATTATTCTACTGCAGTTTTTGCAC; ATGGCTTTCCCACAGTTTGCCGAGAAAGGTGAACAAGGACAGTATCTTCAACAGGATGCT; AATGAATGTTGGATACAAATGATGCGAGTATTGCAACAGAAATTGGAAGCAATAGAGGAT; GATTCTGTTAAAGAGACAGACTCCTCATCTGCATCGGCAGCGACACCTTCTAAAAAGAAA; AGTTTAATCGATCAGTTCTTCGGTGTTGAGTTTGAAACTACCATGAAATGTACAGAATCT; GAAGAAGAAGAAGTCACCAAAGGAAAGGAAAATCAACTTCAGCTTAGCTGTTTTATCAAT; CAGGAAGTCAAGTATCTTTTTACAGGACTTAAATTGCGACTTCAGGAAGAAATCACCAAA; CAGTCTCCAACGTTGCAAAGAAATGCCTTGTATATCAAATCTTCCAAGATCAGCCGGCTG; CCTGCTTACTTGACCATTCAGATGGTTCGATTTTTTTATAAAGAGAAGGAATCTGTGAAT; GCCAAAGTTCTTAAGGATGTTAAATTTCCTCTTATGTTGGATATGTATGAACTGTGTACA; CCAGAACTTCAAGAGAAAATGGTGTCTTTTCGATCCAAATTCAAGGATCTAGAAGATAAA; AAAGTGAATCAGCAGCCAAATACAAGTGACAAAAAGAGTAGTCCCCAGAAAGAAGTTAAG; TATGAACCCTTTTCTTTTGCTGATGATATTGGCTCCAATAATTGTGGATACTATGACTTA; CAAGCAGTACTAACACACCAGGGAAGGTCTAGTTCTTCAGGTCATTATGTATCATGGGTG; AAAAGGAAACAAGATGAATGGATTAAGTTTGATGATGACAAAGTCAGCATCGTAACACCA; GAAGATATCTTACGGCTTTCTGGTGGTGGAGACTGGCATATCGCTTACGTTCTACTCTAT; GGGCCTCGCAGAGTTGAAATAATGGAAGAGGAAAGTGAACAGTAA; ; Clone variation with respect to NM_005151.3;
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005151 unedited; AATTGTATACGACNACTATAGGGCGGCCGCGAATCGGCACGAGGGAGACTCGTCGCACCG; AAGCCGCCGCCACCACCGCGCCTCCGCCTCGGCCGCCGCCGCAGCTGCTCCTGGTCCCCG; TCCCTTTGCCGCCCTCGTCAGGCCCAGCTCTCCTGCGCCGCCGCCTCCCGCCGCGCCCCG; CCATGCCGCTCTACTCCGTTACTGTAAAATGGGGAAAGGAGAAATTTGAAGGTGTAGAAT; TGAATACAGATGAACCTCCAATGGTATTCAAGGCTCAGCTGTTTGCGTTGACTGGAGTCC; AGCCTGCCAGACAGAAAGTTATGGTGAAAGGAGGAACGCTAAAGGATGATGATTGGGGAA; ACATCAAAATAAAAAATGGAATGACTCTACTAATGATGGGGTCAGCAGATGCTCTTCCAG; AAGAACCCTCAGCCAAAACTGTCTTCGTAGAAGACATGACAGAAGAACAGTTAGCATCTG; CTATGGAGTTACCATGTGGATTGACAAACCTTGGTAACACTTGTTACATGAATGCCACAG; TTCAGTGTATTCGTTCTGTGCCTGAACTCAAAGATGCCCTTAAAAGGTATGCAGGTGCCT; TGAGAGCTTCAGGGGAAATGGCTTCAGCGCAGTATATTACTGCAGCCCTTAGAGATTTGT; TTGATTCCATGGATAAAACTTCTTCCAGTATTCCACCTATTATTCTACTGCAGTTTTTGC; ACATGGCTTTCCCACAGTTTGCCGAGAAAGGTGAACAGGGACAGTATCTTCACAGGATGC; TAATGAATGTTGGATACAATGATGCGAGTATTGCACAGAAAATGGAGCATAGAGGATGAT; TCTGTAAGAGAAGACNCTCTCTGCATCGG;
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005151 unedited; GCTATGGCCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAGATGTTATG; ATAATCCCCAAACTTTATTATAAATAACTCAAAATCAGAGCCAAACTGGCTGGTATTCTA; TGCTTTATCCTTTCCCTAGCATCAGTCAAGGGTCCTGGAACAAAGATGAGTAACTATTAA; AATGTAAGAAGCCTATCATATTGAAATACCTCATATGCTTGGTATTTTCTACTATCACTT; TAAAATCACTAAAATTCTGAAACAGACATTAAGATTTGGGAATTTTTTCTTTTTTTTTTT; GAGACAGAGTCTCTCTGTCACCCAGGCTGGAGTGCAGTGGTGTGATCTCAGCTCACTGCA; ACTTCTGCCTTCCAGGTTCAAGCAATTCTCCCACCTCAGCCTCCCAAGTAGCTGGGATCA; CAGGTGTGCACCACCACATCCAGCTAATTTTTATATCTTTAGTAGAGACAGGGTTTCACC; ATGTGGCCAGGCTGGTCTCGAACTCAACCTCCAGTGAACCACCTGCCTCAGCCTCCCAAA; GTGCTGGGATTACAGGCGTGAGCCACCGTGCCCAGCCAAGATTTGGGGAATTAAGAAAGG; AAATACAAACACAACTGAGCCAAGGAAAGAATCTGACTGATAACATCACTGCATTCAGCA; TCCAAAGAAATCCATTACTTTTACAACTTTGTATTTCTTTTTAAATACAGGCAGGCAAAG; AAGATACAAATATTTTTAAATGGATGCTTACAGCACAGTGTTAATAACCATCTTCAGAAA; TTGTACAGTATTAAACATGTCTTCAGCCAAGCTCCACACTCAGAAACAAAAGACCTGAGT; CAAAGCTATTAACCCTTTNTTTTATTATTATAAAGCCTCATTTCTAAAAAAGCACCGAGC; TTGCACATTTCCTAAGACGTTATTCAAGCTTCCAGTTATG;
• Restriction Sites: NotI-NotI
• ACCN: NM_005151
• Insert Size: 3270 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution Method: 1. Centrifuge at 5,000xg for 5min.; 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.; 3. Close the tube and incubate for 10 minutes at room temperature.; 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.; 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.
• Shipping: Ambient

Reference Data

• RefSeq: NM_005151.2, NP_005142.1
• RefSeq Size: 2966 bp
• RefSeq ORF: 1485 bp
• Locus ID: 9097
• UniProt ID: P54578
• Cytogenetics: 18p11.32
• Domains: UBQ, UCH
• Protein Families: Druggable Genome, Protease
• Summary: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]; Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).