USP14 (NM_005151) Human Untagged Clone

CAT#: SC320714

USP14 (untagged)-Human ubiquitin specific peptidase 14 (tRNA-guanine transglycosylase) (USP14), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: USP14
• Synonyms: TGT
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005151.3 GTTTGAATGAGACTCGTCGCACCGAAGCCGCCGCCACCACCGCGCCTCCGCCTCGGCCGC
  CGCCGCAGCTGCTCCTGGTCCCCGTCCCTTTGCCGCCCTCGTCAGGCCCAGCTCTCCTGC
  GCCGCCGCCTCCCGCCGCGCCCCGCCATGCCGCTCTACTCCGTTACTGTAAAATGGGGAA
  AGGAGAAATTTGAAGGTGTAGAATTGAATACAGATGAACCTCCAATGGTATTCAAGGCTC
  AGCTGTTTGCGTTGACTGGAGTCCAGCCTGCCAGACAGAAAGTTATGGTGAAAGGAGGAA
  CGCTAAAGGATGATGATTGGGGAAACATCAAAATAAAAAATGGAATGACTCTACTAATGA
  TGGGGTCAGCAGATGCTCTTCCAGAAGAACCCTCAGCCAAAACTGTCTTCGTAGAAGACA
  TGACAGAAGAACAGTTAGCATCTGCTATGGAGTTACCATGTGGATTGACAAACCTTGGTA
  ACACTTGTTACATGAATGCCACAGTTCAGTGTATTCGTTCTGTGCCTGAACTCAAAGATG
  CCCTTAAAAGGTATGCAGGTGCCTTGAGAGCTTCAGGGGAAATGGCTTCAGCGCAGTATA
  TTACTGCAGCCCTTAGAGATTTGTTTGATTCCATGGATAAAACTTCTTCCAGTATTCCAC
  CTATTATTCTACTGCAGTTTTTGCACATGGCTTTCCCACAGTTTGCCGAGAAAGGTGAAC
  AAGGACAGTATCTTCAACAGGATGCTAATGAATGTTGGATACAAATGATGCGAGTATTGC
  AACAGAAATTGGAAGCAATAGAGGATGATTCTGTTAAAGAGACAGACTCCTCATCTGCAT
  CGGCAGCGACACCTTCTAAAAAGAAAAGTTTAATCGATCAGTTCTTCGGTGTTGAGTTTG
  AAACTACCATGAAATGTACAGAATCTGAAGAAGAAGAAGTCACCAAAGGAAAGGAAAATC
  AACTTCAGCTTAGCTGTTTTATCAATCAGGAAGTCAAGTATCTTTTTACAGGACTTAAAT
  TGCGACTTCAGGAAGAAATCACCAAACAGTCTCCAACGTTGCAAAGAAATGCCTTGTATA
  TCAAATCTTCCAAGATCAGCCGGCTGCCTGCTTACTTGACCATTCAGATGGTTCGATTTT
  TTTATAAAGAGAAGGAATCTGTGAATGCCAAAGTTCTTAAGGATGTTAAATTTCCTCTTA
  TGTTGGATATGTATGAACTGTGTACACCAGAACTTCAAGAGAAAATGGTGTCTTTTCGAT
  CCAAATTCAAGGATCTAGAAGATAAAAAAGTGAATCAGCAGCCAAATACAAGTGACAAAA
  AGAGTAGTCCCCAGAAAGAAGTTAAGTATGAACCCTTTTCTTTTGCTGATGATATTGGCT
  CCAATAATTGTGGATACTATGACTTACAAGCAGTACTAACACACCAGGGAAGGTCTAGTT
  CTTCAGGTCATTATGTATCATGGGTGAAAAGGAAACAAGATGAATGGATTAAGTTTGATG
  ATGACAAAGTCAGCATCGTAACACCAGAAGATATCTTACGGCTTTCTGGTGGTGGAGACT
  GGCATATCGCTTACGTTCTACTCTATGGGCCTCGCAGAGTTGAAATAATGGAAGAGGAAA
  GTGAACAGTAATCTTCATTTTAGTATTTATGCTTAGATGTGAAAATAAATGTTATTTGTT
  GATCATTTCTATAATCCAGAGCTTTAGAGGAAGACACATAGGTGGGTTTATGTTTCACCT
  CATTTGGAACAAAAGAGGACAGAAGCAGACCACTCTGTGCACCAACCTAAAAAATTACAG
  AGAAGAGAAAATTATCTTTGGATTGTGCTGCCCTATATAAAGGTGGCAGAAAGACATTTT
  TAAAAAGCTTATTATTTCTTGCATTATTTTAAAAAGTTCAGAGTTGAAATGCCTTTCAAC
  CATTTCCTTCTGTGGTCATTTTTCTTGCTGCCTTTTTCACCCAAGATTCAGCAGTCAGAT
  GTTTACTGCACACCTATTACCTATTATTTGCTGTTCTTGCATGGTTCAAACCACCATTCT
  GTAGCCACCCATCCTTTGCCTTATCTAACAAACATTTTTCCAGGAAGGTGGAAAAGGAAG
  TGTTGCTCTCATTGTGTGACTCAGTGCTGCTGTCCATCCCATGGAAACATGGGCACAATC
  AAGTATTTGTCCAGCCTATTGCAGGCTTTTCCTGACTTTAAAATAAATTGTGATCAATAA
  TAGTACCTTTGATTATACATTTATTATTGTGTCTCTCTCTGATGTACTGTGGATTGTACA
  TTTAACTTTGGAATGGCTTTGTAATAATCAGTCTTAAGAAAATGTTGACAAGCTCTGGTT
  GCTTATTTTTAGAAAATGAGGACATTTAATAATAATAAAAAAAAAGGGATTAATAGCTTT
  TGACCTCAAGTCTTTTGTCTTCTGAGTGTTGGAGCTTGGCTGAAGACATGTTTAATACTG
  TACAATTTCTGAAGATGGTTATTAACACTGTGCTGTTAAGCATCCATTTAAAAATATTTG
  TTATCTTCTTTGCCTGCCTGTATTTTAAAAAGAAATACAAAGTTGTAAAAGTAATGGATT
  TCTTTGGATGCTGAATGCAGTGATGTTATCAGTCAGATTCTTTCCTTGGCTCAGTTGTGT
  TTGTATTTCCTTTCTTAATTCCCCAAATCTTGGCTGGGCACGGTGGCTCACGCCTGTAAT
  CCCAGCACTTTGGGAGGCTGAGGCAGGTGGTTCACTGGAGGTTGAGTTCGAGACCAGCCT
  GGCCACATGGTGAAACCCTGTCTCTACTAAAGATATAAAAATTAGCTGGATGTGGTGGTG
  CACACCTGTGATCCCAGCTACTTGGGAGGCTGAGGTGGGAGAATTGCTTGAACCTGGAAG
  GCAGAAGTTGCAGTGAGCTGAGATCACACCACTGCACTCCAGCCTGGGTGACAGAGAGAC
  TCTGTCTCAAAAAAAAAAAAAAAAAA
• Restriction Sites: Please inquire
• ACCN: NM_005151
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005151.3, NP_005142.1
• RefSeq Size: 4156 bp
• RefSeq ORF: 1485 bp
• Locus ID: 9097
• UniProt ID: P54578
• Cytogenetics: 18p11.32
• Domains: UBQ, UCH
• Protein Families: Druggable Genome, Protease
• Gene Summary: This gene encodes a member of the ubiquitin-specific processing (UBP) family of proteases that is a deubiquitinating enzyme (DUB) with His and Cys domains. This protein is located in the cytoplasm and cleaves the ubiquitin moiety from ubiquitin-fused precursors and ubiquitinylated proteins. Mice with a mutation that results in reduced expression of the ortholog of this protein are retarded for growth, develop severe tremors by 2 to 3 weeks of age followed by hindlimb paralysis and death by 6 to 10 weeks of age. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (a).