Pdx1 (NM_008814) Mouse Tagged ORF Clone
$289.00
$300.00
In Stock*
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
|---|---|
| Target Symbol | Pdx1 |
| Synonyms | IDX-1; IPF-1; Ipf1; Mody4; pdx-1; STF-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data | |
| Restriction Sites |
SgfI-RsrII Cloning Scheme for this gene
|
Plasmid Map
|
| ACCN | NM_008814 |
| ORF Size | 852 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution Method | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Shipping | Ambient |
| Reference Data | |
| RefSeq | NM_008814.4 |
| RefSeq Size | 1287 bp |
| RefSeq ORF | 855 bp |
| Locus ID | 18609 |
| UniProt ID | P52946 |
| Cytogenetics | 5 86.84 cM |
| MW | 31.4 kDa |
| Summary | Activates insulin and somatostatin gene transcription. Key regulator of islet peptide hormone expression but also responsible for the development of the pancreas, most probably by determining maturation and differentiation of common pancreatic precursor cells in the developing gut. As part of a PDX1:PBX1b:MEIS2b complex in pancreatic acinar cells is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element. Binds the DNA sequence 5'-CCCTTAATGGG-3'.UniProtKB/Swiss-Prot Function |
Reviews
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
"NM_008814" in other vectors (6)
| SKU | Description | Size | Price | |
|---|---|---|---|---|
| MC209160 | Pdx1 (untagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1), (10ug) | 10 ug |
$330.00
|
|
| MG227421 | Pdx1 (tGFP-tagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1), (10ug) | 10 ug |
$489.00
$500.00
|
|
| MR227421L1 | Lenti ORF clone of Pdx1 (Myc-DDK-tagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1) | 10 ug |
$600.00
|
|
| MR227421L2 | Lenti ORF clone of Pdx1 (mGFP-tagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1) | 10 ug |
$600.00
|
|
| MR227421L3 | Lenti ORF clone of Pdx1 (Myc-DDK-tagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1) | 10 ug |
$600.00
|
|
| MR227421L4 | Lenti ORF clone of Pdx1 (mGFP-tagged) - Mouse pancreatic and duodenal homeobox 1 (Pdx1) | 10 ug |
$600.00
|
|
Citations
PDX1 phosphorylation at S61 by mTORC1 links nutrient signaling to β cell function and metabolic disease
Fan, Zhang, Zhang et al
Cell Rep (2026) 45 (1), 116811 DOI: 10.1016/j.celrep.2025.116811
Fan, Zhang, Zhang et al
Cell Rep (2026) 45 (1), 116811 DOI: 10.1016/j.celrep.2025.116811
Cited by 77
PDX1, a transcription factor essential for organ differentiation, regulates SERCA-dependent Ca2+ homeostasis in sensory neurons
Saloman, Epouhe, Ruff et al
Cell Calcium (2024) 120, 102884 DOI: 10.1016/j.ceca.2024.102884
Saloman, Epouhe, Ruff et al
Cell Calcium (2024) 120, 102884 DOI: 10.1016/j.ceca.2024.102884
Cited by 2
AI Summary: OriGene’s CMV-PDX1-Flag plasmid (catalog number MR227421) was used to overexpress PDX1 in Neuro2a cells, enabling the authors to investigate the role of PDX1 in regulating SERCA2B expression and calcium homeostasis in sensory neurons.
Snippet: Neuro2a cells (7 × 105/ per 35 mm petri dish) were transfected with 5 μg CMV-PDX1-Flag (Origene MR227421, Rockville, MD) using TransIT reagent (Mirus Bio, Madison, WI) diluted in Opti-MEM media per the manufacturer's instructions. One day post-transfection, media was replaced with MEM (Ther
Engineered Extracellular Vesicles from Human Skin Cells Induce Pro‐β‐Cell Conversions in Pancreatic Ductal Cells
Ortega-Pineda, Rincon-Benavides, Cuellar-Gaviria et al
Advanced NanoBiomed Research (2023)
Ortega-Pineda, Rincon-Benavides, Cuellar-Gaviria et al
Advanced NanoBiomed Research (2023)
Cited by 4
AI Summary: OriGene's pCMV6 Mouse Tagged ORF Clone for Pdx1 (Myc-DDK tag, MR227421) was used to generate expression plasmids for electrotransfection of human dermal fibroblasts, enabling the production of engineered extracellular vesicles carrying reprogramming factors for pancreatic cell conversion.
Showing 1-3 of 3 papers.
Powered by BizGenius
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen
complexities in the preparation of your product. International customers may expect an additional 1-2 weeks
in shipping.
Related Products
- Price:
- Actual Price:
Welcome to OriGene AI!
I'm your AI-powered guide to everything OriGene.
Ask me anything:
- Product specs
- Ordering help
- Technical questions
- How to find exactly what your experiment needs
Prefer a live agent? Connect with us by clicking the green chat icon, as seen in the image below or email our team at custsupport@origene.com.
